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Morphological and molecular diversity analyses of high biomass sorghum (Sorghum bicolor L. Moench)

By: Tauqeer Ahmad, Ummul Buneen, Syed Naeem Sajid, H. Iqra Almas, Abdul Haseeb, Arzoo Rubab, Maria Kanwal, Qandeel-e-Arsh, Hira Asghar

Key Words: Diversity, PCA, SSR, Polymorphism information content (PIC), Correlation and Biomass.

Int. J. Biosci. 16(3), 253-273, March 2020.

DOI: http://dx.doi.org/10.12692/ijb/16.3.253-273

Certification: ijb 2020 0035 [Generate Certificate]

Abstract

Smart crop sorghum [Sorghum bicolor L. Moench] is the 5th most significant grain crop grown around the globe in marginal land supplementing feedstuff, biofuel and fodder apart from food. Sorghum is considered as an ideal candidate for bioenergy production due to its small genome size, drought tolerance, increased production and potential as biofuel crop. For biomass studies Sorghum germplasm has a higher genetic diversity at molecular and morphological levels so markers assisted breeding has broad applications. To identify genomic diversity of sorghum, SSR markers can be used in breeding techniques. In current research we used morphological characters and molecular markers for evaluating the sorghum genotypes and potential of sorghum to produce a higher yield of biomass. The phenotypic data of diverse germplasm of sorghum for four traits including plant height, stem thickness, number of nodes and internodal length was recorded. PCA analysis specified highly significant differences among all the traits. These traits showed positive correlation and thus used for efficient screening of best performing sorghum accessions. The molecular screening of selected sorghum genotypes was done by using ten SSR primers. The number of alleles per locus ranged from 1 to 2 and the allelic polymorphism information content (PIC) value ranged from 0 to 0.3750 for the Xtxp101. The results revealed that sorghum genotypes differed for morphological characters and this diversity was also indicated by SSR markers. In breeding programs these genomic groups of sorghum may be used for development of efficient energy and high biomass of sorghum.

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Morphological and molecular diversity analyses of high biomass sorghum (Sorghum bicolor L. Moench)

Al-Janbi S, Honeycutt R, Peterson C, Sobral B. 1997. Phylogenetics analysis of organellar DNA sequences in the Andropogoneae: Saccharum. Theoretical and Applied Genetics 88, 933-944.

Assar A, Uptmoor R, Abdelmula A, Salih M, Ordonand F, Friedt W. 2005. Genetic variation in sorghum germplasm from Sudan, ICRISAT, and USA assessed by simple sequence repeats (SSRs). Crop science 45(4), 1636-1644.

Bekele WA, Wieckhorst S, Friedtand WRJ. Snowdon. 2013. High‐throughput genomics in sorghum: from whole‐genome resequencing to a SNP screening array. Plant biotechnology journal 11(9), 1112-1125.

Berenji J, Dahlberg J. 2004. Perspectives of sorghum in Europe. Journal of Agronomy and Crop Science 190(5), 332-338.

Damon E. 1962. The cultivated sorghums of Ethiopia.

De Alencar Figueiredo LF, Calatayud C, Dupuits C, Billot C, Rami JF, Brunel D, Perrier X, Courtois B, Deuand M, Glaszmann JC. 2008. Phylogeographic evidence of crop neodiversity in sorghum. Genetics 179(2), 997-1008.

Doebley J. 1990. Molecular evidence and the evolution of maize. Economic Botany 44(6-27).

Doggett H. 1965. The development of cultivated sorghum. Crop plant evolution, Cambridge University Press, Cambridge, UK.

Fernanda FC, Martha TH, Claudia TG, Zhiwu ZES, Robert LK. Leon, Jurandir VM. 2014. Association mapping provides insight into the origin and the fine structure of the sorghum aluminum tolerance locus, AltSB. PLoSONE 9(1).

Hamblin MT, Fernandez MGS, Casa AM, Mitchell SE, Paterson AH, Kresovich S. 2005. Equilibrium processes cannot explain high levels of short-and medium-range linkage disequilibrium in the domesticated grass Sorghum bicolor. Genetics. 171(3), 1247-1256.

Hariprasanna K, Patil J. 2015. Sorghum: origin, classification, biology and improvement. Sorghum Molecular Breeding. Springer India, India 1, 3-20.

Harlan J, De Wet J. 1972. A simplified classification of cultivated sorghum. Crop science 12(2), 172-176.

Hitchcock AS. 1971. Manual of the grasses of the United States Courier Corporation.

Massiaggia A, Grattapaglia D. 2006. Plant microsettlite genotyping with 4-color fluorescent detection using multiple-tailed primers. Genetic and molecular research: GMR, (5), 72-8.

Murray SC, Rooney WL, Hamblin MT, Mitchell SE, Kresovich S. 2009. Sweet sorghum genetic diversity and association mapping for brix and height. The Plant Genome 2(1), 48-62.

Muui CW, Muasya RM, Kirubi DT, Runoand  SM, Karugu A. 2016. Genetic variability of sorghum landraces from lower Eastern Kenya based on simple sequence repeats (SSRs) markers. African Journal of Biotechnology 15(8), 264-271.

Pakistan Economic Survey. 2015-2016. Govt. of Pakistan, B. o. Statistics, Islamabad.

Paterson AH, Bowers JE, Bruggmann R, Dubchak I, Grimwood J, Gundlach H, Haberer G, Hellsten U, Mitros T, Poliakov A. 2009. The Sorghum bicolor genome and the diversification of grasses. Nature. 457(7229), 551-556.

Rao S, Patil J, Prasad P, Reddy D, Mishra J, Umakanth A, Reddy B,  Kumar AA. 2013. Sweet sorghum planting effects on stalk yield and sugar quality in semi-arid tropical environment. Agronomy Journal. 105(5), 1458-1465.

Reddy RN, Madhusudhana R, Mohan SM, Chakravarthi D, Mehtre S, Seetharama N, Patil J. 2013. Mapping QTL for grain yield and other agronomic traits in post-rainy sorghum [Sorghum bicolor (L.) Moench]. Theoretical and applied genetics 126(8), 1921-1939.

Ritter KB, Jordan DR, Chapman SC, Godwin ID, Mace ES, Mclntyre CL. 2008. Identification of QTLs for suger-related traits in a sweet x and grain sorghum (Sorghum bicolor L. Moench) recombinat inbred population. Molecular Breeding 22, 367-384.

Kimber MS, Pai EF. 2000. The active site architecture of Pisumsativum β‐carbonic anhydrase is a mirror image of that of α‐carbonic anhydrases. The EMBO journal 19(7), 1407-1418.

Khan M, Dubenko I, Stadler S, Ali N. 2008. Magnetostructural phase transitions in Ni50Mn25+ xSb25− x Heusler alloys. Journal of Physics: Condensed Matter, 20(23), 235204.

Xiong J, Tao BW, Qin WF, Tang JL, Han X, Li YR. 2008. Reel-to-reel continuous simultaneous double-sided deposition of highly textured CeO2 templates for YBa2Cu3O7− δ coated conductors. Superconductor Science and Technology 21(2), 025016.

Dhaliwal GS, Jindal V, Dhawan AK. 2010. Insect pest problems and crop losses: changing trends. Indian Journal of Ecology 37(1), 1-7.

Yu J, Buckler ES. 2006. Genetic association mapping and genome organization of maize. Current opinion in biotechnology, 17(2), 155-160.

Ji B, Gao H. 2004. Mechanical properties of nanostructure of biological materials. Journal of the Mechanics and Physics of Solids 52(9), 1963-1990.

Khan M, Fernandes J, Brown K, Simon S, VivotechInc. 2004.Method and apparatus for secure import of information into data aggregation program hosted by personal trusted device. U.S. Patent Application 10, 428-910.

Shiringani AL, Frisch M, Friedt W. 2010. Genetic mapping of QTLs for sugar-related traits in a RIL population of Sorghum bicolor L. Moench. Theoretical and Applied Genetics 121(2), 323-336.

Campbell CJ, Laherrère JH. 1998. The end of cheap oil. Scientific American, 278(3), 78-83.

Muraya DN, Kimamo G. 2011. Effects of cooperative learning approach on biology mean achievement scores of secondary school students’ in Machakos District, Kenya. Educational Research and Reviews 6(12), 726.

Mutegi E, Sagnard F, Semagn K, Deu M, Muraya M, Kanyenji B, Labuschagne M. 2011. Genetic structure and relationships within and between cultivated and wild sorghum (Sorghum bicolor (L.) Moench) in Kenya as revealed by microsatellite markers. Theoretical and applied genetics 122(5), 989-1004.

Tauqeer Ahmad, Ummul Buneen, Syed Naeem Sajid, H. Iqra Almas, Abdul Haseeb, Arzoo Rubab, Maria Kanwal, Qandeel-e-Arsh, Hira Asghar.
Morphological and molecular diversity analyses of high biomass sorghum (Sorghum bicolor L. Moench).
Int. J. Biosci. 16(3), 253-273, March 2020.
https://innspub.net/ijb/morphological-molecular-diversity-analyses-high-biomass-sorghum-sorghum-bicolor-l-moench/
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