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Proximate composition and cytotoxicity of selected sea cucumbers (Holothuroidea) in Goso-on and Vinapor, Carmen, Agusan del Norte, Philippines

By: Archie Galo Layaog, Nenita D. Palmes, Oliva P. Canencia

Key Words: Sea cucumbers, Proximate composition, BSLT, Bioactive compounds, Philippines.

Int. J. Biosci. 14(3), 35-44, March 2019.

DOI: http://dx.doi.org/10.12692/ijb/14.3.35-44

Certification: ijb 2019 0171 [Generate Certificate]

Abstract

Sea cucumbers are seafood that provides potential therapeutic properties, impressive profile of valuable nutrients and unique biological and pharmacological activities (Ozer et al, 2004; Samonte, 2016; Shi et al.,2016).The proximate composition and cytotoxicity of selected sea cucumbers were determined using the sea cucumbers body wall in dry basis. Oven drying was utilized for moisture, ashing for ash, Soxhlet extraction for crude lipid, Kjeldahl for crude protein and by difference for carbohydrates content. Brine shrimp lethality test (BSLT) was used to test the cytotoxicity of the sea cucumbers extracts. Tests were done in triplicate, and an average of each test was noted and expressed in percentages.  Among the three sea cucumbers, B. graeffei had the highest moisture (6.00%) and crude lipid content (1.42%), B. graefffei showed lowest total ash content (33.49%) while A. lecanora had the highest ash content (50.06%) while H. fucopuntata had the highest crude protein (40.74%) and carbohydrates content (39.74%). Moisture content did not show any significant difference (p ˂0.05) among species while significant differences were observed among crude lipid, crude ash, crude protein and carbohydrates (p˂0.05).Holuthuria fuscopunctata extract exhibited the lowest lethal concentration (LC50) in BSLT which was 32.10 ppm. All samples showed high protein and low lipid levels, two sea cucumbers extracts exhibited median lethal concentration (LC50) in BSLT except A. lecanora extract (LC50>1000ppm). The study suggests that results could be linked to the presence of a wide array of bioactive compounds found in sea cucumbers.

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Proximate composition and cytotoxicity of selected sea cucumbers (Holothuroidea) in Goso-on and Vinapor, Carmen, Agusan del Norte, Philippines

Bordbar S, Anwar F, Saari N. 2011. High-value components and bioactives from sea cucumbers for functional foods – A review. Marine Drugs 9, 1761–1805.

https://doi.org/10.3390/md9101761

Bhakuni DS, Rawat DS. 2005. Bioactive metabolites of marine algae, fungi and bacteria. In: Bioactive marine natural products. Co-published by Anamaya Publishers and  Springer, New Delhi, p 1-25.

https://doi.org/10.1007/1-4020-3484-9_1

ChangLee MV, Price RJ, Lampila LE. 1989. Effect Of Processing On Proximate Composition And Mineral Content Of Sea Cucumbers (Parastichopus Spp.). Journal of Food Science 54(3), 567-568.

https://doi.org/10.1111/j.1365-2621.1989.tb04653.x

Chanley JD, Kohn SK, Nigreli RF, Sobotka H. 1955. Further chemical analysis of holothurin, the saponin-like steroid from sea cucumber. Zoologica 40, 99

Chen J. 2003. Overview of sea cucumber farming and ranching practices in China. Beche-de-mer Information Bulletin 18, 18-23.

Chen S, Xue C, Yin L, Tang Q, Yu G, Chai W. 2011. Comparison of structures and anticoagulant activities of fucosylated chondroitin sulfates from different sea cucumbers. Carbohydrate Polymer 83, 688–696. https://doi.org/10.1016/j.carbol.2010.08.040

Choo PS. 2008. Population status, fisheries and trade of sea cucumbers in Asia. FAO fisheries and aquaculture technical paper. InToral-Granda et al.(Ed). Sea Cucumbers in a global review of the fisheries and trade. FAO Fisheries and Acquaculture Technical Paper 516, p 81-118.

Clarkson C, Maharaj VJ, Crouch NR, Grace OM, Pillay P, Matsabisa MG, Bhagwandin N, Smith PJ, Folb PI. 2004. In vitro antiplasmodial activity of medicinal plants native to or naturalized in South Africa. Journal of Ethno pharmacology 2, 177-191.

https://doi.org/10.1016/j.jep.2004.02.011

Conand C, Byrne M. 1993. A review of recent developments in the world sea cucumber fisheries. Marine Fisheries Review 55(4), 1-13.

http://aquaticcommons.org/id/eprint/9859

Erickson KL, Macko SA, Van Dover. 2009. Evidence for a chemoautotrophic ally based food web at an inactive hydrothermal vents (Manus basin). Deep Sea Research Part II: Tropical Studies in Oceanography 56(19-20), 1577-1585.

https://doi.org/10.1016/j.dsr2.2009.05.002

Finney DJ. 1981. Probit analysis, 3rd edition. Cambridge, United Kingdom: Cambridge University Press.

Ginger ML, Billett DSM, Mackenzie KL. 2001. Organic matter assimilation and selective feeding by holothurians in the deep sea; some observation and comments. Progress in Oceanography 50, 407-42.

https://doi.org/10.1016/S0079-6611(01)00063-5

Habermehl G, Volkwein G. 1971. Aglycones of the toxins from the Cuvierian organs of Holothuria forskali and a new nomenclature for the aglycones

form Holothurioideae. Toxicon 9, 319–326.

https://doi.org/10.1016/0041-0101(71)90128-0

Habermelh GG, Krebs HC. 1990 Toxins of Echinoderms. In: Atta-ur Rahman, Ed., Studies in Natural Products Chemistry,Elsiever, Amsterdam 7, 256-316.

Kelly MS. 2005.  Echinoderms:  their culture and   bioactive compounds.  Progress in Molecular and   Subcellular Biology  39, 139­165.

https://doi.org/10.1007/3-540-27683-1_7

Kjeldahl J. 1883. New Method for the Determination of Nitrogen in Organic Substances 22 (1), 366-383

Mamelona JE, Pelletier K, Girard-Lalancette J, Legaut S, Karbourne S, Kermasha O. 2007. Quantification of phenolic contents and antioxidant capacity of Atlantic sea cucumber. Cucumariafrondosa. Food Chemistry 104, 1040–1047.

https://doi.org/10.1016/j.foodchem.2007.01.016

Meyer BN, Ferrigni NR, Putnam JE, Jacobson IB, Nichols DE, Melaughlin JL. 1982. Brine shrimp: A convenient general bioassay for active plant constituents. Plants Medicine 45, 31-34.

https://doi.org/10.1055/s-2007-971236

MINITAB 13.30 (Minitab Inc., State College, PA., USA).

Mokhlesi A, Saeidnia S, Gohari AR,  Shahverdi AR,  Nasrolahi A, Farahani F, Khoshnood R, Es`haghi N. 2012. Biological Activities of the Sea Cucumber Holothurialeucospilota. Asian Journal of Animal and Veterinary Advances 7, 249.

https://doi.org/:10.3923/ajava.2012.243.249

Olawa LF, Nuñeza OM. 2013. Brine shrimp lethality assay of the ethanolic extracts of three selected species of medicinal plants from Iligan City, Philippines. International Research Journal of

Biological Sciences 2(11), 74-77.

O’zer NP, Mol S, Varlik C. 2004. Effect of the procedures on the chemical composition of sea cucumber. Turkish Journal of Fisheries and Aquatic Sciences 4, 71–74.

Raj Alpha M. 2016. Calculation of LD50 or LC50 Using Probit Analysis.

alpharajm@gmail.com

Reid LM, O’Donnell CP, Downey G. 2006. Recent technological advances for determination of food authenticity. Trends in Food Science and Technology 17, 344-353.

https://doi.org/10.1016/j.tifs.2006.01.006

Rivera AP, Uy MM. 2011.In vitro Antioxidant and Cytotoxic Activities of Some Marine Sponges Collected off Misamis Oriental Coast, Philippines 9(1), 354-458.

https://dx.doi.org/10.1155/2012/506046

Samonte PA.  2016. Reviving the Sea Cucumber Industry in the Philippines, Marine Research Division, Department of Science and Technology – Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development of the Department of Science and Technology (DOST-PCAARRD).

Sarhadizadeh N, Afkhami M, Ehsanpour M. 2014 Evaluation bioactivity of Sea cucumber, Stichopushermanni from Persian Gulf. Journal Food Science 67(4), 1319–1322.

Shi S, Feng W, Hu S, Liang S, An N, Mao Y. 2016.Bioactive compounds of sea cucumbers and their therapeutic effects 34(3), P. 549-558.

https://doi.org/10.1007/s00343-016-4334-8

SPC. 2000. Beche-de-mer Information Bulletin 32, 60–61.

Subasinghe S. 1992.Shark fin, sea cucumber and jellyfish. A Processor’s Guide. Info fish Technical Handbook 6, 11-22.

Wang H, Lu X, Yao H, Feng J, Liu R. 2009. Research progress on application of coumarin and its derivatives. Chemical Industry Times 23(8), 40-3.

Wen J, Hu C, Fan S. 2010. Chemical composition and nutritional quality of sea cucumbers. Journal of Science and Food Agriculture 90, 2469–2474.

https://doi.org/10.1002/jsfa.4108

Zao Q, Wang JF, Xue Y, Gao S, Lei M, Xue CHH. 2008 Journal of Fishery Sciences of China 15, 154-159.

Archie Galo Layaog, Nenita D. Palmes, Oliva P. Canencia.
Proximate composition and cytotoxicity of selected sea cucumbers (Holothuroidea) in Goso-on and Vinapor, Carmen, Agusan del Norte, Philippines.
Int. J. Biosci. 14(3), 35-44, March 2019.
https://innspub.net/ijb/proximate-composition-cytotoxicity-selected-sea-cucumbers-holothuroidea-goso-vinapor-carmen-agusan-del-norte-philippines/
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