Proximate composition and cytotoxicity of selected sea cucumbers (Holothuroidea) in Goso-on and Vinapor, Carmen, Agusan del Norte, Philippines

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Research Paper 01/03/2019
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Proximate composition and cytotoxicity of selected sea cucumbers (Holothuroidea) in Goso-on and Vinapor, Carmen, Agusan del Norte, Philippines

Archie Galo Layaog, Nenita D. Palmes, Oliva P. Canencia
Int. J. Biosci.14( 3), 35-44, March 2019.
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Sea cucumbers are seafood that provides potential therapeutic properties, impressive profile of valuable nutrients and unique biological and pharmacological activities (Ozer et al, 2004; Samonte, 2016; Shi et al.,2016).The proximate composition and cytotoxicity of selected sea cucumbers were determined using the sea cucumbers body wall in dry basis. Oven drying was utilized for moisture, ashing for ash, Soxhlet extraction for crude lipid, Kjeldahl for crude protein and by difference for carbohydrates content. Brine shrimp lethality test (BSLT) was used to test the cytotoxicity of the sea cucumbers extracts. Tests were done in triplicate, and an average of each test was noted and expressed in percentages.  Among the three sea cucumbers, B. graeffei had the highest moisture (6.00%) and crude lipid content (1.42%), B. graefffei showed lowest total ash content (33.49%) while A. lecanora had the highest ash content (50.06%) while H. fucopuntata had the highest crude protein (40.74%) and carbohydrates content (39.74%). Moisture content did not show any significant difference (p ˂0.05) among species while significant differences were observed among crude lipid, crude ash, crude protein and carbohydrates (p˂0.05).Holuthuria fuscopunctata extract exhibited the lowest lethal concentration (LC50) in BSLT which was 32.10 ppm. All samples showed high protein and low lipid levels, two sea cucumbers extracts exhibited median lethal concentration (LC50) in BSLT except A. lecanora extract (LC50>1000ppm). The study suggests that results could be linked to the presence of a wide array of bioactive compounds found in sea cucumbers.


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