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Zinc oxide nanoparticles induced histopathological alterations in the spleen of male sprague dawley rats

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Research Paper 01/01/2019
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Zinc oxide nanoparticles induced histopathological alterations in the spleen of male sprague dawley rats

Nagina Rehman, Muhammad Ali, Farhat Jabeen, Azhar Rasool, Muhammad Kashif Zahoor
Int. J. Biosci.14( 1), 300-308, January 2019.
Certificate: IJB 2019 [Generate Certificate]
Key: HistopathologyNano-particlesSpleenZnO

Abstract

Considering the wide spread application of nanotechnology in a number of industries (cosmetics, dyes, rubber and food etc.), extensive role of Zinc oxide nanoparticles (ZnO-NPs) is reported in many fields of biology, but limited information is available regarding their toxic effects. The primary purpose of the current study was to assess the toxic effects of ZnO nano particles (ZnO-NPs) in the spleen of male Sprague Dawley rats via intraperitoneal injection @ of either 10 or 20 or 30 mg/kg bw on alternate day for 28 days. At the end of experiment, rats were sacrificed and spleen was collected for the investigation of metal accumulation and histopathological studies. Histological alterations included magakarycytes, macrophages along with the accumulation of zinc particles, congestion of white pulp and the cell necrosis in ZnO-NPs treated groups and their severity was dose dependent from low to high dose treated groups (10<20<30 mg/kg) in comparison with the control group. Likewise, accumulation of Zinc metal showed significant results within treated groups in an order of low< medium<high (10< 20 < 30 mg/kg). Results of the current study revealed the dose dependent toxicity and accumulation of zinc metal in the organs with their histological alterations in living organisms. So, further studies regarding ZnO toxicity is recommended.

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Aziz A. 2015. Histopathological and immunological changes induced by magnetic nanoparticles in the spleen, liver and genital tract of mice following intravaginal installation. The Journal of Basic and Applied Zoology 71, 32-47. https://doi.org/10.1016/j.jobaz.2015.03.003

Borm PJ, Kreyling W. 2004. Toxicological hazards of inhaled nanoparticles potential implications for drug delivery. Journal of Nanoscience and Nanotechnology 4(5), 521–31.

Braydich-Stolle L, Hussain S, Schlager JJ, Hofmann MC. 2005. In Vitro cytotoxicity of nanoparticles in mammalian germline stem cells. Toxicological Sciences 88(2), 412–9. https://doi.org/10.1093/toxsci/kfi256

Braydich-Stolle LK, Lucas B, Schrand A, Murdock RC, Lee T, Schlager JJ, Hussain SM, Hofmann MC. 2010. Silver nanoparticles disrupt GDNF/Fyn kinase signaling in spermatogonial stem cells. Toxicological Sciences 116(2), 577-89. https://doi.org/10.1093/toxsci/kfq148

Cho WS, Kang BC, Lee Jk, Jeong J, Che JH, Seok SH. 2013. Comparative absorption, distribution and excretion of titanium dioxide and zinc oxide nanoparticles after repeated oral administration. Particle and Fiber Toxicology 10, 9. https://doi.org/10.1186/1743-8977-10-9.

De Berardis B, Civitelli G, Condello M. 2010. Exposure to ZnO nanoparticles induces oxidative stress and cytotoxicity in human colon carcinoma cells. Toxicology and Applied Pharmacology 246, 116–127. https://doi.org/10.1016/j.taap.2010.04.012.

Fujihara J, Miki T, Hashimoto H, Yamada T, Kimura-Kataoka K, Yasuda T, Fujita Y, Takeshita H. 2015. Distribution and toxicity evaluation of ZnO dispersion nanoparticles in single intravenously exposed mice. The Journal of medical investigation 62(1), 45-50. https://doi.org/10.2152/jmi.62.45.

Hackenberg S, Zimmermann  FZ, Scherzed A, Friehs G, Froelich K, Ginzkey C, Koehler C, Burghartz M, Hagen R, Kleinsasser N. 2011. Repetitve exposure to zinc oxide nanoparticles induces dna damage in human nasal mucosa mini organ cultures. Environmental and Molecular Mutagenesis 52(7), 582-9. https://doi.org/10.1002/em.20661

Hillyer JF, Albrecht RM. 2001. Gastrointestinal persorption and tissue distribution of differently sized colloidal gold nanoparticles. Journal of Pharmceutical Sciences 90, 1927-1936. https://doi.org/10.1002/jps.1143

Hussein AJ. 2015. Histopathological study of lung, kidney, spleen and prostate in adult male rats treated with bisphenol A. Basrah journal of veterinary research 4(2), 74-86.

Ibrahim KE, Al-Mutary MG, Bakhiet AO, Khan HA. 2018. Histopathology of the Liver, Kidney, and Spleen of Mice Exposed to Gold Nanoparticles. Molecules 23, 1848. https://doi.org/10.3390/molecules23081848

Johnston HJ, Hutchison G, Christensen FM, Peters S, Hankin S, Stone V. 2010. A review of the in vivo and in vitro toxicity of silver and gold nanoparticles: particle attribute and biological mechanisms responsible for the observed toxicity. Critical Reviews in Toxicology 40(4), 328-46. https://doi.org/10.3109/10408440903453074.

Josefsson EC, James C, Henley KJ. 2011. Megakaryocytes possess a functional intrinsic apoptosis pathway that must be restrained to survive and procedure platelets. The Journal of Experimental Medicine 208, 2017-2031. https://doi.org/10.1084/jem.20110750

Koeneman BA, Zhang Y, Westerhoff P, Chen Y, Crittenden JC, Capco DG. 2010. Toxicity and celluler responses of intestinal cells exposed to titanium dioxide. Cell Biology and Toxicology 26(3), 225-38. https://doi.org/10.1007/s10565-009-9132-z.

Mazen NF, Saleh EZ, Mahmoud AA, Shaalan AA. 2017. Histological and immunohistochemical study on the potential toxicity of silver nanoparticles on the structure of the spleen in adult male albino rats. The Egyptian Journal of Histology 40(3), 374-387. https://doi.org/10.21608/EJH.2017.4662.

Nasu, A, Otsubo Y. 2006. Rheology and UV protection properties of suspensions of fine titanium dioxides in a silicone oil. Journal of Colloid Interface Science 296, 558–564. https://doi.org/10.1016/j.jcis.2005.09.036

Nations S, Wages M, Canas JE, Maul J, Theodorakis C, Cobb GP. 2011. Acute effects of Fe2O3, TiO2, ZnO and CuO nanomaterilas on Xenopus laevis. Chemosphere 83(8), 1053-61. https://doi.org/10.1016/j.chemosphere.2011.01.061.

Plum LM, Rink L, Haase H. 2010. The Essential Toxin: Impact of Zinc on Human Health. International Journal of Environmental Research and Public Health 7(4), 1342-1365. https://doi.org/10.3390/ijerph7041342

Pujalte l, Passagne l, Brouillaud B, Treguer M, Durand E, Ohayon-Courtes C, L’Azou B. 2011. Cytotoxicity and oxidative stress induced by different metallic nanoparticles on human kidney cells. Particle and Fiber Toxicology 8, 10. https://doi.org/10.1186/1743-8977-8-10

Schilling K, Bradford B, Castelli D, Dufour E, Nash JF, Pape W, Schulte S, Tooley I, Van den Bosch J, Schellauf F. 2010. Human safety review of “nano” titanium dioxide and zinc oxide. Photochemical and Photobiological Sciences 9(4), 495-509. https://doi.org/10.1039/b9pp00180h.

Song W, Zhang J, Zhang J. 2010. Acute toxicity of different sized ZnO nanoparticles in mice after intratracheal administration. International Conference on Bioinformatics and Biomedical Engineering. https://doi.org/10.1109/ICBBE.2010.5515207

Teow Y, Asharani PV, Hande MP, Valiyaveettil S. 2011. Health impact and safety of engineered nanomaterials. Chemical Communication (Cambridge, England) 47(25), 7025-38. https://doi.org/10.1039/c0cc05271j.

Toyoshima S, Wakamatsu E, Ishida Y, Obata Y, Kurashima Y, Kiyono H, Abe R. 2017. The spleen is the site where mast cells are induced in the development of food allergy. International Immunology 29(1), 31-45. https://doi.org/10.1093/intimm/dxx005.

Tyner KM, Schiffman SR, Giannelis EP. 2004. Nanobiohybrids as delivery vehicles for camptothecin. Journal of Controlled Release 95(3), 501–14. https://doi.org/10.1016/j.jconrel.2003.12.027

Wang B, Feng W, Wang M, Wang T, Gu Y, Zhu M, Ouyang H, Shi J, Zhang F, Zhao Y, Chai Z, Wang H, Wang J. 2008. Acute toxicological impact of nano-and submicro-scaled zinc oxide powder on healthy adult mice. Journal of Nanoparticles Research 10(2), 263-276. https://doi.org/10.1007/s11051-007-9245-3.

Zheng JL, Yan SS, Shen B, Wu CW. 2017. Organ-specific effects of low-dose zinc pre-exposure on high-dose zinc induced mitochondrial dysfunction in large yellow croaker Pesudosciaena crocea. Fish Psychology and Biochemistry 43(2), 653-661. https://doi.org/10.1007/s10695-016-0319-y.

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