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Zinc oxide nanoparticles induced histopathological alterations in the spleen of male sprague dawley rats

Nagina Rehman, Muhammad Ali, Farhat Jabeen, Azhar Rasool, Muhammad Kashif Zahoor

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Int. J. Biosci.14(1), 300-308, January 2019

DOI: http://dx.doi.org/10.12692/ijb/14.1.300-308


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Considering the wide spread application of nanotechnology in a number of industries (cosmetics, dyes, rubber and food etc.), extensive role of Zinc oxide nanoparticles (ZnO-NPs) is reported in many fields of biology, but limited information is available regarding their toxic effects. The primary purpose of the current study was to assess the toxic effects of ZnO nano particles (ZnO-NPs) in the spleen of male Sprague Dawley rats via intraperitoneal injection @ of either 10 or 20 or 30 mg/kg bw on alternate day for 28 days. At the end of experiment, rats were sacrificed and spleen was collected for the investigation of metal accumulation and histopathological studies. Histological alterations included magakarycytes, macrophages along with the accumulation of zinc particles, congestion of white pulp and the cell necrosis in ZnO-NPs treated groups and their severity was dose dependent from low to high dose treated groups (10<20<30 mg/kg) in comparison with the control group. Likewise, accumulation of Zinc metal showed significant results within treated groups in an order of low< medium<high (10< 20 < 30 mg/kg). Results of the current study revealed the dose dependent toxicity and accumulation of zinc metal in the organs with their histological alterations in living organisms. So, further studies regarding ZnO toxicity is recommended.


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Zinc oxide nanoparticles induced histopathological alterations in the spleen of male sprague dawley rats

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