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Immunotoxic effect of sodium nitrate in immunized mice with Brucella melitensis

Research Paper | October 1, 2018

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Riyam I. Mustafa, Salema Lafta Hassan

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Int. J. Biosci.13( 4), 61-70, October 2018

DOI: http://dx.doi.org/10.12692/ijb/13.4.61-70


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Brucella melitensis causes a worldwide zoonosis. It is one of the major causes of abortions in sheep and goats. The organism is secreted in the milk of infected animals. This study aimed to determine the immunotoxic effects of sodium nitrate and to make a comparison between direct immunization by Brucella vaccine and Vitamin E. A total of forty eight adult males Swiss Albino mice, aged 8 weeks, were divided into four groups as follows: the 1stgroup administrated orally with drinking water 1/10LD50 (273.84mg/kg b.w.) of sodium nitrate daily for 6 weeks and at the same time inoculated by I/P  with 0.4 ml Brucella Rev1 vaccine  two doses for two weeks interval. The 2nd group was immunized and administrated with sodium nitrate as 1st group and at same time  vitamin E (0.15  I.U./kg). The 3rd group was immunized as 1st group. The 4th group was considers as control negative group. Blood  samples were collected from (1st, 2nd, 3rd and 4th) groups for  measuring serum of INF-g and IgG using ELISA Kit. The result showed that the sodium nitrate caused inhibition of both cellular and humoral immune response in addition to decrease the phagocytic activity. Result also, showed that the mean value of skin thickness in G3 at 24 hr. (p<0.05) was a significantly (P<0.05) lower in G2 and in G1 and G4 at 48 hr. Also, the result of ELISA tests for detection levels of (IgG) titer in G3 showed a significant decreasing (p<0.05) compared with G2, G1 and G4. The result of ELISA tests for detection levels of (INF-g) titer in G3 (p<0.05) revealed a significant decreasing (P <0.05) as compared with G2, G1 and G4.


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Immunotoxic effect of sodium nitrate in immunized mice with Brucella melitensis

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