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Impact of carbon sources on callus induction and regeneration ability in banana cv. Sabri

Md. Jamal Hossain, Md. Abdul Bari, Nure Anzuman Ara, Md. Al Amin1, Motiur Rahman, Kazi Md. Faisal Haque

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Int. J. Biosci.3(1), 156-163, January 2013

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Abstract

The purpose of this study was to investigate the effect of three different carbon sources; sucrose, glucose and sorbitol alone and their combinations on in vitro callus induction and regeneration in banana cv. Sabri. Male flowers were used as experimental material in this investigation. The male flowers were cut into small pieces and cultured on MS medium supplemented with 2 mg/l 2, 4-dichloro phenoxy acetic acid (2, 4-D) + 1 mg/l a-naphthalene acetic acid (NAA) + 1 mg/l Indole-3-acetic acid (IAA) + 1 mg/l biotin+1 mg/l glutamine containing different percentages of sugars like sucrose, glucose and sorbitol singly and in their combinations. Glucose showed the highest performance rate for callus induction and 3 % concentration proved the optimal dose. Sucrose and sorbitol behave different in embryo formation and they produced the highest and lowest number of embryos, respectively in regeneration medium. In respect of overall performance the highest percentages shoot and root formation was obtained in the media containing 3% and 2% sucrose, respectively. These findings will be beneficial for the further experiment of other cultivars of banana.

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Impact of carbon sources on callus induction and regeneration ability in banana cv. Sabri

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