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Research Paper | January 1, 2013

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Impact of carbon sources on callus induction and regeneration ability in banana cv. Sabri

Md. Jamal Hossain, Md. Abdul Bari, Nure Anzuman Ara, Md. Al Amin1, Motiur Rahman, Kazi Md. Faisal Haque

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Int. J. Biosci.3(1), 156-163, January 2013


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The purpose of this study was to investigate the effect of three different carbon sources; sucrose, glucose and sorbitol alone and their combinations on in vitro callus induction and regeneration in banana cv. Sabri. Male flowers were used as experimental material in this investigation. The male flowers were cut into small pieces and cultured on MS medium supplemented with 2 mg/l 2, 4-dichloro phenoxy acetic acid (2, 4-D) + 1 mg/l a-naphthalene acetic acid (NAA) + 1 mg/l Indole-3-acetic acid (IAA) + 1 mg/l biotin+1 mg/l glutamine containing different percentages of sugars like sucrose, glucose and sorbitol singly and in their combinations. Glucose showed the highest performance rate for callus induction and 3 % concentration proved the optimal dose. Sucrose and sorbitol behave different in embryo formation and they produced the highest and lowest number of embryos, respectively in regeneration medium. In respect of overall performance the highest percentages shoot and root formation was obtained in the media containing 3% and 2% sucrose, respectively. These findings will be beneficial for the further experiment of other cultivars of banana.


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Impact of carbon sources on callus induction and regeneration ability in banana cv. Sabri

Alina T, Magdalena J, Andrzej T. 2006. The effect of carbon source on callus induction and regeneration ability in Pharbitis nil. Acta Physiologiae Plantarum 28, 619-626.

Bach A, Pawowska B. 1993. Effect of type of carbohydrates in regulation of Hyacinthus orientalis L. in long-term cultures. Folia Horti 2, 3-11.

Blanc G, Lardet L, Martin A, Jacob JL, Carron MP. 2002. Differential carbohydrate metabolism conducts morphogenesis in embryogenic callus of Havea brasiliensis (Müll. Arg.). J. Exp. Bot any 53, 1453-1462.

Bogunia H, Przywara L. 2000. Rola cukrowców w rooelinnych kulturach in vitro. (Sugars in plant tissue culture.) Wiad. Bot 43, 25-36.

Borkowska B, Szczerba J. 1995. Influence of different car bon sources on invertase activity and growth of sour cherry (Pru nus cerasus L.) shoot cul tures. J. Exp. Bot 42, 911-915.

El-Bakry AA. 2002. Effect of genotype, growth regulators, car bon source, and pH on shoot induction and plant regeneration in tomato. In Vitro Cell. Dev. Biol. Plant 38, 501-507.

Fuentes SRL, Calheiros MBP, Manetti-Filho J, Vieira LGE. 2000. The effects of silvernitrate and different carbohydrate sources on somatic embryogenesis in Coffea canephora. Plant Cell Tiss. Org.Cult 60, 5-13.

Gibson SI. 2000. Plant sugar-response path way. Part of complex regulatory web. Plant Physiol 124, 1532-1539.

Hossain MJ, Rahman M, Bari MA. 2007. Establishment of Cell Sulspension Culture and Plantlet Regeneration of Brinjal (Solanum melongena L.). Journal of Plant Sciences 2, 407-415.

Jain, Babbar SB. 2003. Effect of car bon source on the shoot proliferation potential of epicotyl explants of Syzygium cuminii. Biol. Plant 47, 133-136.

Jang JC, Leon P, Zhou L, Sheen J. 1997. Hexokinase as a sugar sensor in higher plant. Plant Cell 9, 5-19.

Kumria R, Waie B, Rajam MV. 2001. Plant regeneration from transformed embryogenic callus of an elite indica rice via Agrobacterium. Plant Cell Tiss. Org. Cult 67, 63-71.

Kutschera U, Heiderich A. 2002. Sucrose metabolism and cellulose biosynthesis in sun flower hypocotyls. Physial. Plant 114, 372-379.

Lemos EEP, Baker DA. 1998. Shoot regeneration in response to carbon source on internodal explants of Annona muricata L. Plant Growth Regul 25, 105-112.

Lipavska H, Konradova H. 2004. Somatic embryogenesis in conifers: The role of carbohydrate metabolism. In Vitro Cell. Biol.-Plant 40, 23-30.

Murashige T, Skoog F. 1962. A revised me dium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15, 473-497.

Nowak BK, Miczyñski, Hudy L. 2004. Sugar up take and utilization during adventitious buds differentiation on in vitro leaf explants of ‘Wêgirka Zwyk³a’ plum (Pru nus domestica) Plant Cell Tiss. Org. Cult 76,255-260.

Peterson KKJ, Hansen, Krogstrup P. 1999. Significance of different carbon source and sterilization methods on callus induction and plant regeneration of Miscanthus x ogiformis Honda ‘Giganteus’. Plant Cell Tiss. Org. Cult 58, 189-197.

Priyakumari I, Sheela VL, George S, Mirsa RL. 2002. Effect of carbon sources on In vitro shoot proliferation and rooting of gladiolus. Floriculture Research Trend In India. Proceedings of the National Symposium on Indian Floriculture in new milliennium, Lal-Bagh, Bangalore, Feb, 2002.

Sairam RV, Franclin G, Hassel R, Smith B, Meeker K, Kashikar, Abes DA, Ismail S, Berry K, Goldman SL. 2003. A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus. Plant Cell Tiss. Org. Cult 75, 79-85.

Strosse H, Van den Houwe I, Panis B. 2004. Banana cell and tissue culture – review. FAO, 2004.

Tang W. 2000. High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panaxginseng. Plant Cell Rep 19, 727–732.

Traore A, Guiltinan M. 2009. Micropropagation of Theobroma cacao using somatic embryo-derived plants. In Vitro Cell Dev Biol Plant 39, 332–337.

Xiao W, Sheen J, J-Ch. Jang. 2000. The role of hexokinase in plant sugar signal transduction and growth and development. Plant Mol. Biol 44, 451-461.


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