Impact of integrated use of bio and mineral nitrogen fertilizers on productivity and profitability of wheat (Triticum aestivum L.) under upper Egypt conditions

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Research Paper 01/12/2013
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Impact of integrated use of bio and mineral nitrogen fertilizers on productivity and profitability of wheat (Triticum aestivum L.) under upper Egypt conditions

Essam A. Abd El-Lattief
Int. J. Agron. Agri. Res.3( 12), 67-73, December 2013.
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The effect of integrated use of mineral N fertilizer and biofertilizer (Azotobacter and/or Azospirillum) on grain and straw yields, harvest index and net profit of wheat was assessed. A field experiment was carried out during two years on a sandy soil. The recommended N (230 kg N ha-1) and biofertilizer (Azotobacter and Azospirillum) were applied alone and in various combinations among them. A randomized complete block design, with three replications, was used in this study. Treatments significantly affected grain and straw yields, and harvest index. The highest values of such traits were obtained in treatment T11 (75% mineral N + biofertilizer with Azotobacter and Azospirillum). However, T12 (50% mineral N + biofertilizer with Azotobacter and Azospirillum) resulted also higher values for the above mentioned traits comparing with T1 (100% mineral nitrogen and uninoculated) but the differences among the two treatments almost did not attain the statistical differences. In addition, T11 and T12 gave the maximum return and net profit per ha. From this study, it can be concluded that the biofertilizers (double-inoculation of Azotobacter and Azospirillum) of efficient strains could save 25 or 50 % of the recommended dose of mineral N.


Abbasdokht H. 2008. The study of Azotobacter chroococcum inoculation on yield and post harvest quality of wheat. International Meeting on Soil Land Management and Agroclimatology, Turkey, 885-889.

Abd El-Lattief EA. 2008. Increasing bread wheat (Triticum aestivum L.) productivity and profitability in the newly reclaimed lands through the integrated use mineral, organic and bio-fertilizers. Alexandria Journal of Agricultural Research 53, 47-54.

Badr A, Elham OM, Ibrahim El-Kramany MF. 2009.  Interaction  effect  of  biological  and  organic fertilizers on yield and yield components of two wheat cultivars. Egyptian Journal of Agronomy 31, 17-27.

Bahrani AJ, Pourreza Hagh Joo M. 2010. Response of Winter Wheat to Co-Inoculation with Azotobacter and Arbescular Mycorrhizal Fungi (AMF) under Different Sources of Nitrogen Fertilizer. American-Eurasian Journal of Agricultural & Environmental Science 8, 95-103.

Choudhury ATMA, Kennedy IR. 2004. Prospects and potentials for systems of biological nitrogen fixation in sustainable rice production. Biology and Fertility of Soils 39, 219–227.

Choudhury ATMA, Kennedy IR. 2005. Nitrogen fertiliser losses from rice soils and control of environmental pollution problems. Communications in Soil Science and Plant Analysis 36, 1625–1639.

De Datta SK, Buresh RJ. 1989. Integrated nitrogen management in irrigated rice. Advances in Soil Science 10, 143–169.

Dobereiner J, Day JM. 1975. Nitrogen fixation in the rhizosphere of tropical grasses. In: Nitrogen fixation by free living microorganisms. WDP Stewart (ed.). Cambridge Univ. Press, London, 39-56.

El-Garhi AS, Atia NA, Sara Fouda EE. 2007. Effect of inoculating N-fixing bacteria (Cerealine) on wheat (Triticum aestivum, L) growth and nutrient content. Zagazig Journal of Agricultural Research 34, 249-273.

El-Sayed MZ, Abd El-Sattar AE, Basha HA, Abd El-Hammeed IM. 2005. Improvement of wheat productivity in newly reclaimed soil in Egypt. Annals UMCS, Sec. E. 60, 113-121.

Fayez M, Emam NF, Makboul HE. 1985. The possible use of nitrogen fixing Azospirilum as biofertilizer for wheat plants. Egyptian Journal of Microbiology 20, 199-206.

Hafeez FY, Hameed S, Zaidi AH, Malik KA. 2002. Biofertilizers for Sustainable Agriculture. In: Techniques for Sustainable Agriculture, 67-73. ISBN, NIAB, Faisalabad, Pakistan.

Jen-Hshuan Chen. 2006. The combined use of chemical and organic fertilizers and or biofertilizer for crop growth and soil fertility. In: International Workshop on Sustained Management of the Soil-Rhizosphere System for Efficient Crop Production and Fertilizer Use. Land Development Department, Bankok-10900, Thailand. October, 16-20, 125-130.

Kennedy IR, Choudhury ATMA, Kecskés ML. 2004. Non-symbiotic bacterial diazotrophs in cropfarming systems: can their potential for plant growth promotion be better exploited? Soil Biology and Biochemistry 36, 1229–1244.

Khavazi K, Asadi-Rahmani H, Malakouti MJ. (Eds.). 2005. Necessity for the production of biofertilizers in Iran. Ministry of Jihad-e- Agriculture, Agricultural Research and Education Organization (AREO) and Soil and Water Research Institute (SWRI), Tehran, Iran, 419.

Lakshami K, Subba NS, Tilak KV, Singh CS. 1972. Azospirillum, a new bacterial fertilizer for tropical crops. Sci Reporter, Council of Scientific and Industrial Research (India), 16, 690-692.

Mahato P, Anoop B, Chauhan JS. 2009. Effect of Azotobacter and Nitrogen on Seed Germination and Early Seedling Growth in Tomato. Researcher 1, 62-66.

Narula N, Yadav KS. 1989. Nitrogen Fixation Research in India with Azotobecter. In: Biological Nitrogen Fixation Research Status in India, Dadarwal KR, Yadav KS (Eds.). Indian society for Plant Physilogy and Biochemistry, New Delhi, India, 88-124.

Narula N, Gupta PP, Kumar PR, Laxminarayan K. 1993. Field response of Indian mustard (Brassica juncea) to inoculation of soil isolate and analogue resistant mutant of Azotobacter chrooccocum. Annals of Biology Indiana 9, 144-148.

Radwan SMA, Hussein HF. 1996. Effect of bio and organic fertilization on wheat yield under different weed control treatments. Egyptian Journal of Applied Science 11, 267-281.

Rai SN, Caur AC. 1998. Characterization of Azotobacter Spp. and effect of Azospirilum lipoferum on the yield and N-uptake of wheat crop. Plant and Soil 109, 131-134.

Sharief AE, El-Kalla SE, Leilah AA, Mostafa HEM. 1998. Response of wheat cultivars to nitrogen fertilizer levels and biological fertilization. Mansoura University Journal of Agricultural Science 23, 5807-5816.

Steel RGD, Torrie JH. 1980. Principles and procedures of statistics, 2nd ed McGraw-Hill, New York.

Tawfik MM, Gomaa AM. 2005. Effect of organic and biofertilizers on the growth and yield of wheat plants. Egyptian Journal of Agricultural Research 2, 711-725.

Tilak KVBR. 1992. Azospirillum brasilense and Azotobacter chrooccocum inoculum effect of mayze and sorghum. Soil Biology and Biochemistry 14, 417-418.