Impact of thermal stress on survival and induced cross tolerance to toxins of Bacillus thuringiensis in wild Aedes aegypti

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Research Paper 01/07/2017
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Impact of thermal stress on survival and induced cross tolerance to toxins of Bacillus thuringiensis in wild Aedes aegypti

T. Sarita Achari, Usha Rani Acharya, Tapan Kumar Barik
Int. J. Biosci.11( 1), 156-164, July 2017.
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To assess the impact of thermal stress, late third instar larvae of field collected Aedes aegypti were exposed to variable temperatures viz. 39°C, 40°C, 41°C, 42°C, 43°C, 44°C and 45°C. All larvae were survived up to 300 minutes exposure at 39°C where as hundred percent larval mortality observed at higher temperature exposure. Further, it was observed that 25.1%, 35.6%, 78.9%, 90% and 100% larval mortality found after Bti treatment at 0.5 ppm, 1.0 ppm, 1.5 ppm, 2.0 ppm and 2.5 ppm respectively. Similarly, in order to assess the cross tolerance level to Bti, larvae pre-adapted at 39°C for 60 min, 90 min and 120 min duration were re-exposed to Bti solution of 1.0 and 1.5 ppm. Data suggested that the pre-adapted larvae showed 4.4 and 1.9 fold less larval mortality that indicates temperature play an important role in the development of cross tolerance to toxins of Bti in wild Aedes aegypti.


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