Improvement of arbuscular mycorrhizal fungi inoculum production by nutrient solution concentration and soil texture variation

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Research Paper 01/05/2015
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Improvement of arbuscular mycorrhizal fungi inoculum production by nutrient solution concentration and soil texture variation

S.T. Okiobé, M. Abossolo Angue, B.P. Bougnom, Boyomo Onana, D. Nwaga
Int. J. Agron. Agri. Res.6( 5), 7-20, May 2015.
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Arbuscular Mycorrhizal Fungi (AMF) can increase the yield of plants from 50 to 200 %, but the large scale multiplication of AMF inocula is difficult because of their trophic nature, and this represents a big challenge in tropical areas. Within the framework of the study, two separate experiments were conducted in order to determine the most favorable conditions for AMF spore production. The effect of Rorison’s nutrient solution (0, 4 and 8 ml/l) was tested on 6 types of AMF strains for spores production, while in the second experiment; it was the effect of soil-sand mixture variation (5-57 % clay content) that was evaluated in AMF spores production. A local variety of Sorghum bicolor was used as the host plant during the trials. Experiments were performed in completely randomized design with 4 replicates. Results showed that Rorison’s nutrient solution and soil texture significantly (P<0.05) influenced plant growth, symbiotic and biochemical parameters. Nutrient solution induced significant increase in root colonization (5 to 36 %), and AMF spore production (12 to 23 spores/g of soil). The highest concentration of Rorison’s nutrient solution promoted more spore formation, but that was not translated in plant yield. Soil texture variation had a significant impact on AMF root colonization and spores production, since mixture of sand and clay, with clay variation from 20-43% was found to favor both parameters. These data suggest that soil texture variation and nutrient solution concentration can significantly improve AMF spores production and Sorghum symbiotic performances.


Bradford MM. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of Protein-Dye Binding. Analytical Biochemistry 2, 248-254.

Carrenho R, Sandra FBT, Vera LRB, Eraldo SS. 2007. The effect of different soil properties on arbuscular mycorrhizal colonization of peanuts, sorghum and maize. Acta Bot Bras 21, 723-730.

Covacevich  F,  Echeverría  HE,  Pagano  MC. 2012. Arbuscular mycorrhizal fungi: Essential belowground organisms for earth life but sensitive to changing environment. African Journal of Microbiology Research 6, 5523-5535.

Dalpé Y, Aiken S. 1997. Arbuscular mycorrhizal fungi associated with Festuca species in Canadian high Arctic. Can. J. Bot 54, 43-49.

Davamani V, Lourduraj AC, Yogalakshmi RP, Velmurugan M. 2010. VAM in Nutrient Uptake of Crop Plants. in: Thangadurai, D., C.A. Busso., M. Hijri, Eds. Mycorrhizal Biotechnology. Science Publishers, India, 33-43.

Douds JrDD, Schenk NC. 1990. Increased sporulation of Vesicular-Arbuscular Mycorrhizal fungi by manipulation of Nutrient Regimens. Applied and Environmental Microbiology 50, 413-418.

Douds Jr DD, Reider C. 2003. Inoculation with mycorrhizal fungi increases the yield of green peppers in a high P soil. Biological Agriculture and Horticulture 21, 91–102.

Douds JrDD, Nagahashi G, Pfeffer PE, Reider C, Kayser WM. 2006. On-farm production of AM fungus inoculum in mixtures of compost and vermiculite. Bioresource Technology 97, 809–818.

Douds JrDD, Nagahashi G, Reider C, Hepperly PR. 2007. Inoculation with arbuscular mycorrhizal fungi increases the yield of potatoes in a high P soil. Biological Agriculture and Horticulture 25, 67–78.

Ebrahim S, Nasser A. 2013. Influence of Glomus etunicatum and Glomus intraradices fungi inoculums and micronutrients deficiency on root colonization and dry weights of tomato and sorghum in perlite bed culture. African Journal of Biotechnology 12, 3957-3962.

Gaur A, Adholeya A. 2000. Effects of particle size of soil-less substrates upon AM fungus inoculum production. Mycorrhiza 10, 43-48.

Hawkins HJ, George E. 1997. Hydroponic culture of the mycorrhizal fungus Glomus mosseae with Linum usitatissmum L., Sorghum bicolor L. and Triticum aestivum L. Plant Soil 196, 143-149.

Hung LLL, Sylvia DM. 1988. Production of vesicular arbuscular mycorrhizal fungus inoculum in aeroponic culture. Applied Environmental Microbiology 154, 353-357.

Kormanik PP, Mc G, AC. 1982. Quantification of vesicular–arbuscular mycorrhizae in plant roots. In: Schenck, N.C, Eds. Methods and Principles of Mycorrhizal Research. The American Phytopathological Society, St Paul, MN, 37-45.

Liu A, Hamel C, Hamilton RI, Ma BL, Smith DL. 2000. Acquisition of Cu, Zn, Mn, and Fe by mycorrhizal maize (Zea mays L.) growing in soil at different P and micronutrient levels. Mycorrhiza 9, 331-338.

Maia LC, Kimbrough JW. 1998. Ultrastructural studies of spores and hypha of a Glomus species. International Journal of plant Science 159, 581-589.

Millner P, Kitt DG. 1992. The Beltsville method for soiless production of vesicular arbuscular fungi. Mycorrhiza 2, 9-15.

Monther MT, Kamaruzaman S. 2012. Mycorrhizal Fungi and Abiotic Environmental Conditions Relationship. Research Journal of Environmental Science 6, 125-133.

Ngonkeu MEL. 2003. Biodiversité et potentiel des champignons mycorhiziens à arbuscules de quelques zones agro-écologiques du Cameroun. Thèse de Doctorat 3e cycle, Université de Yaoundé I, Cameroun, 258.

Nwaga D, Thé C, Ambassa-Kiki R, Ngonkeu Mangaptché EL, Tchiegang-Megueni C. 2004. Selection of arbuscular mycorrhiza fungi for inoculating maize and sorghum grown on Oxisol/Ultisol and Vertisol in Cameroon, in: Bationo A, Eds. Managing nutrient cycles to sustain soil fertility in sub-saharan Africa. Acad. Science Pub. (ASP), TSBF-CIAT, 467-486.

Nwaga D, Jansa J, Abossolo, AM, Frossard E. 2010. The potential of soil beneficial micro-organisms for slash-and-burn agriculture in the humid forest zone of sub-saharan Africa, in: Dion P, Eds. Soil Biology and Agriculture in the Tropics. Springer Heidelberg Dordrecht. London, New York, 80-107.

Nwaga D, Tenkouano A, Tomekpe K, Fogain R, Kinfack DM, Tsané G, Yombo O. 2011. Multi-functional properties of mycorrhizal fungi for crop production: the case study of banana development and drought tolerance. In: Bationo, A., Boaz W., Jeremiah, M., Okeyo, Fredah, M., Job Kihara.J, Eds. Innovations as Key to the Green Revolution in Africa. Vol. 1. Springer Heidelberg Dordrecht. London, New York, 523-531.

Olfat K, Khara J. 2012. Spore density and root colonisation by arbuscular mycorrhizal fungi in some species in the northwest of Iran. International Research Journal of Applied and Basic Science 5, 977-982.

Pawlowska TE, Douds DD, Charvat I. 1999. In vitro propagation and life cycle of the arbuscular mycorrizal fungus Glomus etunicatum. Mycology Research 103, 1549-1556.

Plenchette C, Fortin JA, Furlan V. 1983. Growth responses of several plant species to mycorhizae in a soil of moderate P fertility. Plant and Soil. 70, 199-209.

Richer de Forges, Feller C, Jamagne M, Arrouays M. 2008. Perdus dans le triangle des textures. in: Etudes et gestions des sols.vol 15. France, Montpellier 97-111.

Saito M, Oba KT. 2011. Effect of nitrogen on the sporulation of arbuscular mycorrhizal fungi colonizing several gramineous plant species. Soil Science and Plant Nutrition 57, 29-34.

Sawaki H, Saito M. 2001. Expressed genes in the extraradical hyphae of an arbuscular mycorrhizal fungus, Glomus intraradices, in the symbiotic phase. FEMS Microbiology Letter 195, 109-113.

Schenck NC. 1982. Methods and Principles of Mycorrhizal Research. American Phytopathological Society, St Paul, Minnesota, USA. 244 p.

Silva FSB, Yano-Melo AM, Brandão JAC, Maia LC. 2005. Sporulation of arbuscular mycorrhizal fungi using Tris-hcl buffer in addition to nutrient solutions. Brazilian Journal of Microbiology 36, 327-332.

St-Arnaud M, Hamel C, Vimard B, Caron M, Fortin JA. 1996. Enhanced hyphal growth and spore production of the arbuscular mycorrhizal fungus Glomus intraradices in  an  in vitro  system  in  the absence of the host roots. Mycology Research 100, 328-332.

Sylvia DM, Jarstfer AG. 1994. Production of inoculum and inoculation with arbuscular mycorrhizal fungi. in: Robson, A.P., Abbott, L.K., Malajczek, N, Eds. Management of Mycorrhizas in Agriculture, Horticulture and Forestry. Kluver Academic Press. The Netherlands, 231-238.

Wu CG, Liu YS, Hung LL, 1995. Spore development of Entrophospora kentinensis in an aeroponic system. Mycologia 87, 582-587.

Yeasmin T, Parmita Z, Ataur R, Nurul A, Nurus SK. 2007. Arbuscular mycorrhizal fungus inoculum production in rice plants. African Journal of Agricultural Research 2, 463-467.