Welcome to International Network for Natural Sciences | INNSpub

Improving the germination of somatic embryos in date palm Berhi cultivar in vitro

Research Paper | January 1, 2016

| Download 2

Hussein J. Shareef, Khaun A. Muhsen, Abdulrahman D. Alhamd

Key Words:

Int. J. Agron. Agri. Res.8( 1), 17-23, January 2016


IJAAR 2016 [Generate Certificate]


Embryogenic suspension cultures of Date palm (Phoenix dactylifera L.) allow mass propagation of somatic embryos; Partial desiccation (0, 1, 2, 3 and 4 hours) and low temperature (0°C for 2 hr, 0°C for 4 hr, 4°C for 24 hr and 4°C for 48 hr) treatments were applied to improve germination of somatic embryos in vitro of date palm cultivar Berhi with or without AC. The highest germination percentage was achieved when embryos were desiccated for three hours as well as treatment of low temperature in 4°C for 24 hr. Also, the results proved that found activated charcoal) AC) in liquid media produced the highest somatic embryos number and weight and improving percentage of germination. Further, Partial desiccation and low temperature increased embryos proline content. The improvement of the germination of somatic embryos via low temperature and especially via partial desiccation embryos somatic is successful can be used for the purpose of commercial propagation especially for Berhi cultivar.


Copyright © 2016
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Improving the germination of somatic embryos in date palm Berhi cultivar in vitro

Al-Khayri JM. 2010. Somatic embryogenesis of date palm (Phoenix dactylifera L.) improved by coconut water. Biotechnol 9, 477-484.

Anwar MA, Hossain Md, Anamul H, David JB, Masayuki F. 2014. Proline Protects Plants Against Abiotic Oxidative Stress: Biochemical and Molecular Mechanisms P. Ahmad (Ed): Oxidative Damage to Plants Elsevier Inc 477-522.

Cohen Y. 2011. Molecular detection of somaclonal variation in date palm-In: Jain, S.M., Al-Khayri, J.M., Johnson, D.V. (ed.): Date Palm Biotechnology pp. 221-235. Springer, Dordrecht 2011.

El-Hadrami I, El-Bellaj M, El-Idrissi A, J’Aiti F, El-Jaafari S, Daaf F. 1998. Biotechnologie végétalers et amelioration du palmier dattier (Phoenix dactylifera L.) Pivot de l’agriculture oasienne Marocaine. Cah. Agric 7, 463-468.

Fki L, Masmoudi R, Drira N, Rival A. 2003. An optimised protocol for plant regen-eration from embryogenic suspension cultures of date palm, Phoenix dactyliferaL., cv. Deglet Nour. Plant. Cell. Rep. 21, 517–524.

Fki LN, Bouaziz1, O Chkir1, R Benjemaa-Masmoudi1, A Rival, R Swennen, N Drira1, B, Panis3. 2013. Cold hardening and sucrose treatment improve cryopreservation, BIOLOGIA PLANTARUM 57 (2), 375-379, 2013 of date palm meristems.

Ibraheem YI, Pinker and M, Böhme. 2013. A comparative study between solid and liquid cultures relative to callus growth and somatic embryo formation in date palm (Phoenix dactylifera L.) cv. Zaghlool, Emir. J. Food Agric 25(11), 883-898.

Irigoyen JJ, Emerich DW, Sanchez- Diaz M. 1992. Water stress induce changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiol Plant 84, 55- 60.

Jain MS, Al Khayri JM, DVF J. 2011. Date Palm Biotechnology, 1st ed. Springer Netherlands.

Kermode AR, Dumbroff EB, Bewley JD. 1989. The role of maturation drying in the transition from seed development to germination. J. Exp. Bot 40, 303-313.

Kushnarenko SV, Romadanova NV, Reed BM. 2009. Cold acclimation improves regrowth of cryopreserved apple shoot tips-CryoLetters 30, 47-54.

Othmani A, Bayoudh C, Drira N, Marrakchi M, Trifi M. 2009a. Somatic embryo-genesis and plant regeneration in date palm Phoenix dactylifera L., cv. Boufeggousis significantly improved by fine chopping and partial desiccation of embryo-genic callus. Plant Cell Tiss. Org 97, 71–79.

Othmani A, Bayoudh C, Drira N, Trifi M. 2009 b. In vitro cloning of date palm Phoenix dactylifera L. cv. Deglet Bey by using embryogenic suspension and temporary immersion bioreactor (TIB). Biotechnol. Biotechnol. Eo 23(2), 1181-1188.

Pociecha E, P1azek A, Janowiak F, Zwierzykowski Z. 2009. ABA level, proline and phenolic concentration, and PAL activity induced during cold acclimation in androgenic Festulolium forms with contrasting resistance to frost and pink snow mould (Microdochium nivale). – Physiol. mol. Plant Pathol. 73, 126-132.

Sharma DR, Kumari R, Chowdhury JB. 1980. In vitro culture of female date palm (Phoenix dactylifera L.) tissues. Euphytica 29, 169–174.

Zaid A, Arias-Jimenez EJ. 2002. Date palm cultivation. FAO Plant production andprotection paper 156 Rev. 1. F.A.O Rome.