In-silico analysis to identify role of 3′-UTR associated miRNAs in epilepsy syndromes
Paper Details
In-silico analysis to identify role of 3′-UTR associated miRNAs in epilepsy syndromes
Abstract
Epilepsy is a heterogeneous neurodevelopmental condition. Studying the expression profiles of regulatory molecules that may help to understand the pathogenesis of epilepsy and their regulatory control mechanism is important. MicroRNAs (miRNA) are small noncoding RNA molecules that control gene expression at post-transcriptional level. miRNA associated with untranslated regions (UTRs) of genes may alter the expression in the disease pathogenesis. The objective of present study was to predict the association of different miRNAs in the regulation of genes responsible for different types of epilepsies. In this study, we predicted some common miRNAs associated with 3′-UTR regions of about 30 selected epilepsies causative genes using RegRNA 2.0 an in-silico tool. The more common miRNA that have target sites within regulatory region in number of genes, were hsa-miR-644b-5p, hsa-miR-5193, hsa-miR-4449, hsa-miR-4646-3p, hsa-miR-4739, hsa-miR-766-3p, hsa-miR-2355-5p, hsa-miR-3943, hsa-miR-5006-3p, hsa-miR-574-5p, hsa-miR-4459, hsa-miR-298 and hsa-miR-4728-5p. Different validation scores were obtained from miRmap software, online. Further we analyzed the conservation of UTRs of the selected genes in the higher order mammals like, chimpanzee, gibbon, gorilla and macaca, and observed interesting results. The results of our study reinforce hypothesis that common miRNA may control the expression of different genes involved in epilepsies and regulate the function of respective protein. It may be proposed that miRNAs have role in the pathogenesis of epilepsy.
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Epilepsy genes, miRNAs, RegRNA2, In-silico analysis, miRmap (2017), In-silico analysis to identify role of 3′-UTR associated miRNAs in epilepsy syndromes; IJB, V11, N5, November, P337-345
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