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In vitro activity of selected ruminant Sera on Trypanosoma brucei brucei Plimmer & Bradford, 1899

Felicite Djieyep-Djemna, Ishaya Haruna Nock, Thelma Aken’Ova, Ezekiel Kogi, Armand Claude Noundo Djieyep

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Int. J. Agron. Agri. Res.19(1), 24-30, July 2021


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This study aimed at evaluating the in vitro Trypanosoma brucei brucei activity of sera from some ruminants viz: West African dwarf goat, Red Sokoto goat, Sahel goat, Yankassa sheep, Uda sheep, Balami sheep, N’Dama cattle, Sokoto Gudali cattle, Friesian cross/Bunaji cattle, White Fulani cattle. Blood samples from donor ruminants were screened using thick film and haematocrit centrifugation technique (HCT). to ensure that they were free from trypanosomes. Trypanosoma brucei brucei (Federe strain) was suspended in RPMI supplemented with FCS. After addition of each test serum, the motility of the parasite was used as index of viability. Among the sera from selected small ruminants, only the West African Dwarf goat serum exhibited some anti-trypanosomal activities in vitro, with a slight inhibition index/Anti-trypanosomal Activity Index (ATI) of 0.29, and a significant reduction (P<0.05) in survival time of 21.33±1.15 hours compared with that of all the sera from other small ruminants including the control (30.00±0.00 hours). On the other hand, among the sera from selected large ruminants, only the N’Dama cattle serum showed some anti-trypanosomal activities in vitro, with an inhibition index/ATI of 0.38, and a significant reduction (P<0.05) in survival time of 18.67±1.15 hours compared with that of all the sera from other large ruminants including the control (30.00±0.00 hours). The in vitro anti-trypanosomal activity of sera from West African Dwarf goat and the N’Dama cattle was cidal in nature. The in vitro anti-trypanosomal activity of West African Dwarf goat serum appeared to be related to the xanthine oxidase (XO) activity; the highest ATI of this serum coincided with the highest XO content. This study shows that sera from West African Dwarf goat and N’Dama cattle have innate immunity against T. brucei brucei.


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In vitro activity of selected ruminant Sera on Trypanosoma brucei brucei Plimmer & Bradford, 1899

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