In vitro acute toxicity and bioaccumulation of manganese in common carp fish (Cyprinus carpio)

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Research Paper 01/06/2017
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In vitro acute toxicity and bioaccumulation of manganese in common carp fish (Cyprinus carpio)

Zeeshan Ali, Ali Muhammad Yousafzai, Ijaz Muhammad, Gul-E-Nayab, Syed Abdul Maajid Aqeel, Syed Toheed Shah
Int. J. Biosci.10( 6), 160-165, June 2017.
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The present research and experimental study were conducted out in order to know bioaccumulation of manganese in the gills, intestine and muscles and its acute toxicity to the Common carp (Cyprinus carpio) fingerlings by using manganese sulphate solution. Atomic Absorption Spectrophotometer (Model: Analyst 700, Parkin Elmer, USA, Serial No: 700S5040102) was used for determination of concentration of Manganese (Mn) in the Skin, Intestine and Gills tissue. During experiment common carp fingerlings were exposed to a specific concentration of manganese sulphate solution. In the experiment manganese sulphate solution of 5.6 ppm were applied to 24, 48, 72 and 96 hours exposures of fingerlings to the specific solution. Highest concentration of manganese was in found in gills that is21.6, 26.4, 14.4, 10.32 in gills, 5.49, 3.33, 5.85, 6.96 in intestine and 3.99, 2.22, 2.37, 2.55µg/g in muscles respectively after 24, 48, 72 and 96 hours. Gills, intestine, muscles were not looking much affected and were easily extracted for sampling, but the last one fish were having more affected viscera as compared to the previous fishes which indicated that the fingerling exposed to 96 hours of exposure got much affected by manganese sulphate solution. With increasing of exposure time the morphometric and behavioral changes and abnormalities became more prominent. Out of total 10 common carp fingerlings no mortality occurs all were remained alive. The above observations proved that manganese is toxic to common carp fingerlings and other fishes as well as animals when it increases from its normal and optimal limit.


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