In vitro anthelmintic activity of Areca catechu (Betel nut) seed ethanolic extract against (Ascaridia galli) in native chickens
Paper Details
In vitro anthelmintic activity of Areca catechu (Betel nut) seed ethanolic extract against (Ascaridia galli) in native chickens
Abstract
Ascaridia galli is a common intestinal parasite in chickens, causing significant health issues and economic losses. Conventional anthelmintics are expensive and prone to resistance, necessitating the search for alternative treatments. This study evaluated ethanolic betel nut (Areca catechu) seed extract (BNSEE) kills A. galli in a lab setting. Five treatments were used on nematodes that had just been collected from native chickens that had been slaughtered: T0 (levamisole, positive control), T1 (1 ml BNSEE), T2 (1.5 ml BNSEE), T3 (2 ml BNSEE), and T4 (2.5 ml BNSEE). Worm mortality was assessed at 1, 3, and 6 hours. Statistical analysis (p< 0.05) revealed significant differences among treatments, while time interval and treatment × time interaction effects were not significant. The highest death rates were seen in T0 (10%) and T4 (9.33), but there was no significant difference between them. This suggests that BNSEE at 2.5 ml has comparable anthelmintic activity to levamisole. Lower BNSEE concentrations (1–2 ml) resulted in significantly lower mortality rates, demonstrating a dose-dependent response. Phytochemical analysis confirmed the absence of tannins, saponins, alkaloids, flavonoids, and glycosides in betel nut seeds. These findings suggest that BNSEE is a surprising due to the alkaloids in betel nuts. Further, in vivo studies are recommended to evaluate its safety, efficacy, and potential application in poultry parasite management.
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Al-Amin U. Abrasa, Kent S. Musong, Zandro O. Perez (2025), In vitro anthelmintic activity of Areca catechu (Betel nut) seed ethanolic extract against (Ascaridia galli) in native chickens; IJB, V26, N4, April, P61-67
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