In vitro anthelmintic activity of crude extracts of ethnobotanically important wild plants against Haemonchus contortus (Rudolphi 1803)
Paper Details
In vitro anthelmintic activity of crude extracts of ethnobotanically important wild plants against Haemonchus contortus (Rudolphi 1803)
Abstract
Our preceding ethnobotanical studies revealed that Aerva javanica, Cistanche tubulosa, Cuscuta campestris, Heliotropium europaeum and Persicaria glabra have been used in folk medicines in Sahiwal Division, Punjab, Pakistan. In the present study, different doses of the methanol, chloroform, n-hexane and aquous extracts of these plants were evaluated for their anthelmintic activity against the very devastating blood sucking macro-parasites of goats and sheep ‘Haemonchus contortus’. The serial dilution method was used to determine the most effective dose of the above samples to kill 100% of adult Haemonchus contortus under in vitro conditions. Methanolic, chloroform, n-hexane and aquous crude extracts of five plants were diluted to give four dilutions (10, 20, 50 and 100 mg /mL) each. Non-polar solvent-based extracts were found more effective than that of the polar solvent-based extracts. Chloroform extracts of Cistanche tubulosa, Cuscuta campestris and Persicaria glabra while methanolic extracts Aerva javanica and Cuscuta campestris were found the most effective. Among all Cuscuta campestris demonstrated maximum efficacy providing 100% H. Contortus control within first hour of treatment however other four plants were found to have adequate anthelmintic activity. Helminthiasis is a common disease found in sheep and goats in Asia. Due to increased resistance in heliminths against chemical control, plant extracts are being preferred as an alternate remedy. All these plants particularly Cuscuta campestris warrants future investigations to identify and isolate the possible phytochemicals, responsible for its excellent anthelmintic activity.
Ali A. 2010. Ethnobotanical Studies of Sahiwal Division. M.Phil. Thesis, Department of Botany, Government College University, Lahore.
Badar N, Iqbal Z, Khan MN, Ahhtar MS. 2011. In vitro and in vivo anthelmintic activity of Acacia nilotica (L.) Willd. Bark and leaves. Pakistan Veterinary Journal 31, 185-191.
Blot WJ, Li JY, Taylor RR, Guo W, Dawsey S, Wang GQ, Yang CS, Zheng SF, Gail M, Yu Y, Liu BQ, Tangrea J, Sun YH, Liu YH, Fraumeni FS, Shang YH, LiB. 1993. Nutrition intervention trials in Linxian, China: supplementation with specific vitamin mineral combinations, cancer incidence, and disease specific mortality in the general population. Journal of National Cancer Institute 85, 1483-1492.
Chagas ACS, Vieira LS. 2007. Azadirachta indica (Neem) action in goats gastrointestinal nematodes. Brazilian Journal Veterinary Research and Animal Science 44, 49–55.
Costa CTC, Morais SM, Bevilaqua CML, Souza MMC, Leite FKA. 2002.Ovicidal effect of Mangifera indica L. seeds extracts on Haemonchus contortus. Brazilian Journal of Veterinary Parasitology 11, 57–60
Eguale T, Tilahun G, Debella A, Feleke A, Makonnen E. 2007. In vitro and in vivo anthelmintic activity of crude extracts of Coriandrum sativum against Haemonchu scontortus. Journal of Ethnopharmacology 110, 428-433.
Fasiuddin A, Campbell WC. 2000. Haemonchus contortusis much more sensitive than Caenorhabditis elegans to ovicidal action of Thiabendazole. Journal of Parasitology 86, 629-630.
Ferreira LE, Castro PMN, Chagas ACS, Franca SC, Beleboni RO. 2013. In vitro anthelmintic activity of aqueous leaf extract of Annona muricata L. (Annonaceae) against Haemoncus contortus from sheep. Experimental Parasitology 134, 327-332.
Haraguchi H, Kataoka S, Okamoto S, Mand H, Shibata K. 1999. Antimicrobial triterpenes from Ilex integra and the mechanism of antifungal action. Phytotherapia Residence 13, 151-156.
Hounzangbe-Adote MS, Paolini V, Fouraste I, Moutairou K, Hoste H. 2005. In vitro effects of four tropical plants on three life-cycle stages of the parasitic nematode Haemonchus contortus. Research in Veterinary Science 78, 155–160.
Kamaraj AC, Rahuman A. 2011. Efficacy of anthelmintic properties of medicinal plant extracts against Haemonchus contortus. Research in Veterinary Science 91, 400–404.
Kiros A, Tadesse B, Aylate A, Tadesse B. 2016. Prevalence of Fasciolosis in small ruminants and associated risk factor in and around Kombolcha. The Journal of Advances in Parasitology 3, 61-65.
Kursat M, Erecevit P. 2009. The antimicrobial activities of methanolic extracts of some Lamiaceae members collected from Turkey. Turkish Journal of Science and Technology 4, 81-85.
Peneluc T, Domingues LF, Almeida GN, Ayres MC, Moreira EL, Cruz AC, Bittencourt TCB, Almeida MA, Batatinha MJ. 2009. Anthelmintic activity of aqueous extract of Zanthoxylum rhoifolium Lam. leaves (Rutaceae). Revista Brasileirade Parasitologia Veterinaria 18, 43–48.
Powers KG, Wood IB, Eckert J, Gibson T, Smith HJ. 1982. World associations of the advancement of veterinary parasitology (W.A.A.V.P.) guidelines for evaluating the efficacy of anthelmintics in ruminants (bovine and ovine). Veterinary Parasitology 10, 265–284.
Thompson DP, Geary TG. 1995. The structure and function of helminth surfaces in Biochemistry and Molecular Biology of parasites (J.J. Marr, Ed.), 1st Ed. Acdemic press, New York pp. 203-232.
Vasconcelos ALC, Bevilaqua CM, Morais SM, Maciel MV, Costa CT, Macedo IT, Oliveira LM, Braga RR, Silva RA, Vieira LS. 2007. Anthelmintic activity of Croton zehntneri and Lippia sidoides essential oils. Veterinary Parasitology 148, 288–294.
Vidyadhar S, Saidulu M, Gopal TK, Chamundeeswari D, Rao U, Banji D. 2010. In vitro anthelmintic Activity of the whole plant of Enicostem malittorale by using various extracts. International Journal of Applied Biology and Pharmaceutical Technology 1, 1119-1125.
Ahmad Ali, Faiz Ahmad Joyia, Khalid Farooq Akbar, Zaheer-ud-Din Khan (2016), In vitro anthelmintic activity of crude extracts of ethnobotanically important wild plants against Haemonchus contortus (Rudolphi 1803); JBES, V9, N1, July, P310-318
https://innspub.net/in-vitro-anthelmintic-activity-of-crude-extracts-of-ethnobotanically-important-wild-plants-against-haemonchus-contortus-rudolphi-1803/
Copyright © 2016
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0