In vitro Anti-inflammatory Activity of Nephrolepis biserrata (Sw.) Schott Rhizome and Angiopteris palmiformis (Cav.) C. Chr. Leaf Extracts

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Research Paper 20/08/2022
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In vitro Anti-inflammatory Activity of Nephrolepis biserrata (Sw.) Schott Rhizome and Angiopteris palmiformis (Cav.) C. Chr. Leaf Extracts

Aileen May G. Ang, Edsel Tan, Rainear A. Mendez, Melania M. Enot, Jessa May B. Ofima, Reggie Y. Dela Cruz, Gina B. Barbosa
Int. J. Biosci.21( 2), 279-286, August 2022.
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Increasing inflammation-mediated health issues have driven the search for more natural anti-inflammatory drug sources. In this study, the anti-inflammatory activity of Nephrolepis biserrata rhizome and Angiopteris palmiformis frond extracts were determined via inhibition of pro-inflammatory enzymes, 15-lipoxygenase (15-LOX) and cyclooxygenase-2 (COX-2). Extraction with absolute ethanol was done followed by subsequent partitioning with hexane, ethyl acetate, and water. Results revealed that the ethyl acetate-soluble partition (Nb-EtOAc) and aqueous partition (Nb-Aq) of N. biserrata and the ethanolic extract (Ap-EtOH) of A. palmiformis exhibit active inhibition against the 15-LOX enzyme. All of the N. biserrata extracts (Nb-EtOH, Nb-Hex, Nb-EtOAc, and Nb-Aq) and the hexane-soluble partition (Ap-Hex) of A. palmiformis were found to be active and selective towards inhibition of the COX-2 enzyme. The observed anti-inflammatory activity of N. biserrata rhizome and A. palmiformis frond extracts suggests that N. biserrata rhizomes and A. palmiformis fronds are potential sources of natural anti-inflammatory components.


Aggarwal B, Shishodia S, Sandur S, Pandey M, Sethi G. 2006. Inflammation and cancer: How hot is the link? Biochemical Pharmacology 72(11), 1605-1621.

Ahmed A. 2011. An overview of inflammation: mechanism and consequences. Frontiers in Biology 6(4), 274-281.

Auerbach B, Kiely J, Cornicelli J. 1992. A Spectrophotometric Microtiter-Based Assay for the Detection of Hydroperoxy Derivatives of Linoleic Acid. Analytical Biochemistry 201(2), 375-380.

Axelrold B, Cheesebrough T, Laakso S. 1981. Lipoxygenase from Soybeans. Methods in Enzymology Lipids Part C. 71, 441-451.

Bosma-den Boer M, van Wetten ML, Pruimboom L. 2012. Chronic Inflammatory diseases are stimulated by current lifestyle: how diet, stress levels and medication prevent our body from recovering. Nutrition and Metabolism 9(1), 1-14.

Chudzik M, Korzonek-Szlacheta I, Krol W. 2015. Triterpenes as Potentially Cytotoxic Compounds. Molecules 20(1), 1610-1625.

de Winter WP, Amoroso VB. 2003. Cryptogams: ferns and fern allies. Plant Resources of South-East Asia – Backhuys Publishers 15(2).

Fry M, Bonner A. 2012. Development of a fluorescent-based assay to detect cyclooxygenase inhibitory activity of ūĚõŅ-lactone derivatives. 22nd Annual Argonne Symposium for Undergraduates. Central States Incorporated, Argonne National Laboratory, Argonne, Illinois, USA.

Komala I, Yardi A, Betha OS, Muliati F, Ni’mah M. 2015. Antioxidant and anti-inflammatory activity of the Indonesian ferns, Nephrolepis falcata and Pyrrosia lanceolata. International Journal of Pharmacy and Pharmaceutical Sciences 7(12), 162-165.

Kormin F, Khan M, Shafie NSM, Nour AH, Yunus RM. 2018. Statistical mixture design: Study of solvent performance in temperature-controlled microwave assisted extraction system on antioxidant properties of N. biserrata (Schott.) Sw. frond extract. International Journal of Engineering and Technology 7(3.7), 166-172.

Lamichhane R, Pandeya PR, Lee KH, Kim SG, Devkota HP, Jung HJ. 2020. Anti-Adipogenic and Anti-Inflammatory Activities of (‚ąí)-epi-Osmundalactone and Angiopteroside from Angiopteris helferiana C. Presl.¬†Molecules¬†25(6), 1337.

Ofoego EU. 2015. The phytochemical analysis and evaluation of the antioxidant and antidiabetic potentials of ethanolic leaf extract of Nephrolepis biserrata. Thesis submitted to the Department of Biotechnology Technology, FUTO.

Otsuka H. 2006. Purification by Solvent Extraction Using Partition Coefficient. Methods in Biotechnology 20, 269-273.

Rainsford KD. 1999. Profile and mechanisms of gastrointestinal and other side effects of non-steroidal anti-inflammatory drugs (NSAIDs). The American Journal of Medicine 107(6A), 27S-35S.

Schumacher M, Juncker T, Schnekenburger M, Gaascht F, Diederich M. 2011. Natural compounds as inflammation inhibitors. Genes and Nutrition 6(2), 89-92.

Shah MD, Yong YS, Iqbal M. 2014. Phytochemical investigation and free radical scavenging activities of essential oil, methanol extract and methanol fractions of N. biserrata. International Journal of Pharmacy and Pharmaceutical Sciences 6(9), 269-277.

Shorinwa OA, Ogeleka NO. 2016. Antinociceptive and Anti-inflammatory Activities of Aerial Part of N. biserrata (Sw) Schott 5, 246‚Äď254

Zhang L, Virgous C, Si H. 2019. Synergistic anti-inflammatory effects and mechanisms of combined phytochemicals. The Journal of nutritional biochemistry 69, 19-30.