In vitro antibacterial potential of Withania coagulans Dunal (Solanaceae)
Paper Details
In vitro antibacterial potential of Withania coagulans Dunal (Solanaceae)
Abstract
The present study was conducted to evaluate the antibacterial potential of W. coagulansDunal. Using agar well diffusion method. Three different crude extracts (ethyl acetate, chloroform and aqueous) of leaves stem and roots were tested against four pathogenic bacterial strains at four different concentrations (0.5, 1.0, 1.5, 2.0 mg/ml). Results show that maximum inhibition had been shown by W. coagulansethyl acetate root extract at 2mg/ml concentration with 24mm, 20mm, 19mm and 23mm zone of inhibition against Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis and Klebsiella pneumonia respectively. Chloroform leaves extracts were also most effective against all the four pathogenic bacterial strains. Minimum inhibition potential had been shown by aqueous leaves extract (2mg/ml) with 3-11mm zone of inhibition against all tested pathogenic bacterial strains. Furthermore, ethyl acetate and aqueous stem extract (2mg/ml) had almost similar effect against S. aureus (18.35 and 18.02mm inhibition zone). Chloroform leaves extract (2mg/ml) and ethyl acetate stem extract had considerable inhibition against the selected bacterial pathogens. Chloramphenicol (standard antibiotic) was much effective against S. aureus and K. pneumoniawith 26.69mm and 25.46 mm zone of inhibition. The overall study determined the medicinal importance of W. coagulans.
Bonjar S. 2004. Evaluation of antibacterial properties of some medicinal plants used in Iran. Journal of Ethnopharmacology 94(2-3), 301-305. http://dx.doi.org/10.1016/j.jep.2004.06.007
Ahmad I, Beg AZ. 2001. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. Journal of Ethno pharmacology 74(2), 113-123. http://dx.doi.org/10.1016/S0378-8741(00)00335-4
Kunin CM. 1993. Resistance to antimicrobial drugs—a worldwide calamity. Annals of internal medicine 118(7), 557-561. http://dx.doi.org/10.7326/0003-4819-118-7-199304010-00011
Scheck AC, Perry K, Hank NC, Clark WD. 2006.Anticancer activity of extracts derived from the mature roots of Scutellaria baicalensis on human malignant brain tumor cells. BMC Complementary and Alternative Medicine 6(1), 27. http://dx.doi.org/10.1186/1472-6882-6-27
Shad AA, Shah HU, Bakht J. 2013.Ethnobotanical assessment and nutritive potential of wild food plants. Journal of Animal and Plant Sciences 23(1), 92-7.
Erasto P, Adebola PO, Grierson DS, Afolayan AJ. 2005. An ethnobotanical study of plants used for the treatment of diabetes in the Eastern Cape Province, South Africa. African Journal of Biotechnology 4(12). http://dx.doi.org/pjbs.2008.2051.2053
Gupta V, Keshari BB. 2013. Withania coagulans Dunal. (Paneer Doda): A Review. International Journal of Ayurvedic and Herbal Medicine3(5), 1130-1136.
Agosta W. 1996. Bombardier beetles and fever trees: a close-up look at chemical warfare and signals in animals and plants. Addison Wesley Publishing Company, New York. P. 224.
Ramasamy TS, Yu JS, Selden C, Hodgson H, Cui W. 2012. Application of three-dimensional culture conditions to human embryonic stem cell-derived definitive endoderm cells enhances hepatocyte differentiation and functionality. Tissue Engineering Part A 19(3-4), 360-367. http://dx.doi.org/10.1089/ten.tea.2012.0190
Edeoga HO, Okwu DE, Mbaebie BO. 2005. Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology 4(7), 685-688. http://dx.doi.org/10.5897/AJB2005.000-3127
Desideri D, Meli MA, Roselli C. 2010. Determination of essential and non-essential elements in some medicinal plants by polarised X-ray fluorescence spectrometer (EDPXRF). Microchemical Journal 95(2), 174-180. http://dx.doi.org/10.1016/j.microc.2009.11.010
Gupta S, Walia A, Malan R. 2011. Phytochemistry and pharmacology of Cedrus deodera: an overview. International Journal of Pharmaceutical Sciences and Research 2(8), 2010.
Harborne JB. 1973. Phytochemical methods, London Chapman and Hall, Ltd 49-88.
Heinrich M, Ankli A, Frei B, Weimann C, Sticher O. 1998. Medicinal plants in Mexico: Healers’ consensus and cultural importance. Social Science & Medicine 47(11), 1859-1871.
Dias DA, Urban S, Roessner U. 2012. A historical overview of natural products in drug discovery. Metabolites 2(2), 303-336. http://dx.doi.org/10.3390/metabo2020303
Qin G W, Xu RS. 1998. Recent advances in bioactive natural products from Chinese medicinal plants. Medicinal research reviews 18(6), 375-382. http://dx.doi.org/10.1007/s11434-015-0929-2
Shahid W, Durrani R, Iram S. 2013. Antibacterial activity in Vitro of medicinal plants. Sky Journal of Microbiology Research 1, 5-21.
Kirthikar KR, Basu BD. 1933. Indian Medicinal Plants.2d ed. Bishen Singh Mahendra Pal Singh, Delhi.
Naz A. 2002. Studies on The Chemical Constituents Withania Coagulans and Boswellia Dalzielli (Doctoral dissertation, University of Karachi).
Budhiraja RD, Bala S, Garg KN. 1977.Pharmacological investigations on fruits of Withania coagulans, Dunal. Planta Medica 32 (06), 154-157. http://dx.doi.org/10.1055/s-0028-1097575
Maurya R, Singh AB, Srivastava AK. 2008. Coagulanolide, a with anolide from Withania coagulans fruits and antihyperglycemic activity. Bioorganic & medicinal chemistry letters 18(24), 6534-6537. http://dx.doi.org/10.1016/j.bmcl.2008.10.050
Bonjar GHS, Farrokhi PR. 2004.The antibacillus activity of some plants used in traditional medicine of Iran. Niger. Journal of Natural Products 8, 34-39. http://dx.doi.org/10.4314/njnpm.v8i1.11811
Mughal T, Naeem I, Qureshi S,Abass A. 2010. Synergistic antibacterial studies of Heliotropium sterigosum. Journal of Applied Pharmaceutical Science 1(2), 27-36.
Choudhary MI, Nawaz SA, Lodhi MA, Ghayur MN, Jalil S, Riaz N,Gilani AH. 2005. Withanolides, a new class of natural cholinesterase inhibitors with calcium antagonistic properties. Biochemical and biophysical research communications 334(1), 276-287.
Khan MTJ, Ashraf M, Tehniyat S, Bukhtair MK, Ashraf S, Ahmed W. 1993. Anti-bacterial activity of Withania coagulans. Fitoterapia 64, 367.
Gaind KN, BudhirajaRD. 1967. The antibacterial and anthelmintic activity of Withania coagulans Dunal. Indian Journal of Pharmaceutical Sciences 29, 185-186.
Sudhanshu MS, Rao N, Menghani E. 2012.The phytochemical and antimicrobial activity of Withania coagulans (Stocks) Dunal (Fruit). International Journal of Pharmacy and Pharmaceutical Sciences 4, 387-389.
Wright CW. 2005. Plant-derived antimalarial agents: new leads and challenges, Phytochemistry Reviews 4(1), 55–61. http://dx.doi.org/10.1007/s11101-005-3261-7
Abd Ullah, Adnan Khan, 2018. In vitro antibacterial potential of Withania coagulans Dunal (Solanaceae). J. Biodiv. Environ. Sci., 12(6), 139-146.
Copyright © 2018 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.