In vitro antibacterial potential of Withania coagulans Dunal (Solanaceae)

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Research Paper 01/06/2018
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In vitro antibacterial potential of Withania coagulans Dunal (Solanaceae)

Abd Ullah, Adnan Khan
J. Bio. Env. Sci.12( 6), 139-146, June 2018.
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The present study was conducted to evaluate the antibacterial potential of W. coagulansDunal. Using agar well diffusion method. Three different crude extracts (ethyl acetate, chloroform and aqueous) of leaves stem and roots were tested against four pathogenic bacterial strains at four different concentrations (0.5, 1.0, 1.5, 2.0 mg/ml).  Results show that maximum inhibition had been shown by W. coagulansethyl acetate root extract at 2mg/ml concentration with 24mm, 20mm, 19mm and 23mm zone of inhibition against Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis and Klebsiella pneumonia respectively. Chloroform leaves extracts were also most effective against all the four pathogenic bacterial strains. Minimum inhibition potential had been shown by aqueous leaves extract (2mg/ml) with 3-11mm zone of inhibition against all tested pathogenic bacterial strains. Furthermore, ethyl acetate and aqueous stem extract (2mg/ml) had almost similar effect against S. aureus (18.35 and 18.02mm inhibition zone). Chloroform leaves extract (2mg/ml) and ethyl acetate stem extract had considerable inhibition against the selected bacterial pathogens. Chloramphenicol (standard antibiotic) was much effective against S. aureus and K. pneumoniawith 26.69mm and 25.46 mm zone of inhibition. The overall study determined the medicinal importance of W. coagulans.


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