In vitro anticancer activity of small peptides from Tridax procumbens

Paper Details

Research Paper 01/09/2018
Views (343) Download (30)

In vitro anticancer activity of small peptides from Tridax procumbens

Choppavarapu Hari Krishna, Kasturi Kondapalli
Int. J. Biosci.13( 3), 180-184, September 2018.
Certificate: IJB 2018 [Generate Certificate]


Cancer is a hyper proliferative disorder and one of the most devastating diseases in both developing and developed countries. Chemotherapeutic drugs are enormously expensive and are associated with serious side effects. Still, the search continues for an ideal treatment that has minimal side effects and cost-effective. Over 50% of drugs in clinical trials for anti-cancer activity were isolated from natural plant sources. Plant species are enriched with potential anticancer proteins and peptides. Tridax procumbens phytochemicals having antiseptic, insecticidal, parasiticidal, anticancer properties, but the anticancer properties of peptides was not investigated. Thus the present investigation was aimed to evaluate anticancer activity of small peptides (SPs) from T. procumbens. The leaf, stem and flowers were shade dried and extracted with acetonitrile and formic acetic acid (50:1). The extracted SPs were purified by HPL Cand the purified SPs were tested for its cytotoxicity activity by MTT (-[3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyl tetrazolium bromide)] assay against human breast cancer metastatic cell line (MDA MB 231). Among all the tested SPs, leaf SPs showed lowest IC50with 4µg/ml. In conclusion, the SPs showed potent cytotoxicity against breast cancer which can be further characterised.


Roa GV, Kumar SS, Islam M, Mansour SE. 2008. Folk medicines for anticancer therapy- a current statuse. Cancer The raphy 6, 913-922.

Newman DJ, Cragg GM, Snader KM. 2003. Natural products as sources of new drugs over the period 1981-2002. Journal of Natural Products 66, 1022-1037.

Shirisha R, Bibechana T, Varalakshmi KN. 2014. Evalution of the anticancer potentials of Origanum marjorana on fibro sarcoma (HT_1080) cell line. Asian Pacific Journal of Tropical Diseases 4, S389-S394.

Fu LL, Zhou CC, Yao, Yu JY, Liu B, Bao JK. 2011. Plant lectins: targeting programmed cell death pathways as antitumor agents. International Journal  of Biochemistry & Cell Biology 43, 1442-1449.

Damme EJV, Peumans WJ, Barre A, Rougé P. 1998. Plant Lectins: A Composite of Several Distinct Families of Structurally and Evolutionary Related Proteins with Diverse Biological Roles. Critical Reviews in Plant Sciences 17, 575–692.

Nia R, Paper DH, Essien EE, Oladimeji OH, Iyadi KC, Franz G. 2003. Investigation into in-vitro radical scavaging and in-vivo anti inflammatory potential of Tridax procumbens. Nigerian Journal of Physiological Science18, 39-43.

Sampson B, Barlow GB. 1980. Separation of peptides and amino acids by ion-exchange chromatography of their copper complexes. Journal Of Chromatography B: Biomedical Sciences and Applications 183, 9-15.

Mant CT, Chen Y, Yan Z, Popa TV, Kovacs JM, Mills BP, Tripet RS. 2007. Hodges. “HPLC analysis and purification of peptides. Methods in Molecular Biology 386, 3-55.

Raymond S, Weintraub L. 1959. Acrylamide gel as a supporting medium for zone electrophoresis. Science 130, 711.

Hansen MB, Nielsen SE, Berg K. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. Journal of Immunological Methods 119, 203-210.

Sara AA, Hany NB, Ahmed SI, Ahmed AG, Farid AB. 2014. Anticancer activity of plant-derived proteins against human tumor cell lines. Journal of Drug Discovery and Therapeutics 2, 60-69.