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Research Paper | April 1, 2020

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In vitro anticancer effect of Aquilaria crassna extract on human mammary gland cancer cells

Hye Myoung Jang, Ji Hye Lee, Ju Hyeun Kim, Garam Park, Gwang J. Jeon

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Int. J. Biosci.16(4), 187-192, April 2020

DOI: http://dx.doi.org/10.12692/ijb/16.4.187-192


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Agarwood has long been known for its therapeutic effects. In the current study, anticancer effects of Aquilaria crassna extracts (ACE) on human breast cancer in vitro with MDA-MB-231 and MCF-7 cancer cells were studied. Cell survival and cell death were investigated mainly by Immunofluorescence (IF) assay. The gene expressions of PCNA and BCL-2 for cancer cell proliferation, Caspase-3 for apoptosis and VEGF for angiogenesis were detected. The Hoechst analysis was also made to confirm the degradation of nucleus membrane. The cancer cells treated with ACE showed effects of cytotoxicity with significant increase in Caspase-3 expression in the treated group compared to the control. The potential blood vessel formation induced by VEGF was significantly lower in the cancer cells treated with ACE. Also, MMP2 were highly expressed. We concluded that the Aquilaria crassna extract showed anticancerous effects in human mammay gland cells.


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In vitro anticancer effect of Aquilaria crassna extract on human mammary gland cancer cells

Abbas P, Hashim YZH, Salleh HM. 2018. Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells. Journal of Research in Pharmacology 23, 120-129. https://doi.org/10.12991/jrp.2018.116

Abbas P, Hashim YZHY, Salleh HM. 2018. Cytotoxic effects and response surface optimization of solvent extraction of crude extracts from Aquilaria subintegra uninfected branch. Science Heritage Journal 12, 10 -15. http://doi.org/10.26480/gws.02.2018.10.15

Adam AZ, Lee SY, Mohamed R. 2017. Pharmacological properties of agarwood tea derived from Aquilaria (Thymelaeaceae) leaves: An emerging contemporary herbal drink. Journal of Herbal Medicine 10, 37-44. https://doi.org/10.1016/j.hermed.2017.06.002

Dahham SB, Tabana YM, Hassan LEA, Ahamed MBK, Majid ASA, Majid AMSA. 2016. In vitro antimetastatic activity of Agarwood (Aquilaria crassna) essential oils against pancreatic cancer cells. Alexandria Journal of Medicine 52, 141-50. http://dx.doi.org/10.1016/j.ajme.2015.07.001

Hara H, Ise Y, Morimoto N, Shimazawa M, Ichihashi K, Ohyama M, Iinuma M. 2008. Laxative Effect of Agarwood Leaves and Its Mechanism. Bioscience Biotechnology and Biochemistry 72, 335–345. https://doi.org/10.1271/bbb.70361

Hashim YZ, Phirdaous A, Azura A. 2014. Screening of anticancer activity from agarwood essential oil. Pharmacology Research 6, 191–194. https://doi.org/10.4103/0974-8490.132593

Khalil AS, Rahim AA, Taha KK, Abdallah KB. 2013. Characterization of Methanolic Extracts of Agarwood Leaves. Journal of Applied Industrial Science 3, 78-88.

Miyoshi T, Ito M, Kitayama T, Isomori S, Yamashita F. 2013. Sedative Effects of Inhaled Benzylacetone and Structural Features Contributing to Its Activity. Biology Pharmcology Bulletin 36, 1474–1481. https://doi.org/10.1248/bpb.b13-00250

Okugawa H, Ueda R, Matsumoto K, Kawanishi K, Kato A. 1993. Effects of Agarwood Extracts on the Central Nervous System in Mice. Planta Medicina 59, 32-36. https://doi.org/10.1055/s-2006-959599

Takemoto H, Ito M, Shiraki T, Yagura T, Honda G. 2008. Sedative effects of vapor inhalation of agarwood oil and spikenard extract and identification of their active components. Journal of Natural Medicine 62, 41–46. https://doi.org/10.1007/s11418-007-0177-0


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