Int. J. Biosci.16(4), 187-192, April 2020
Agarwood has long been known for its therapeutic effects. In the current study, anticancer effects of Aquilaria crassna extracts (ACE) on human breast cancer in vitro with MDA-MB-231 and MCF-7 cancer cells were studied. Cell survival and cell death were investigated mainly by Immunofluorescence (IF) assay. The gene expressions of PCNA and BCL-2 for cancer cell proliferation, Caspase-3 for apoptosis and VEGF for angiogenesis were detected. The Hoechst analysis was also made to confirm the degradation of nucleus membrane. The cancer cells treated with ACE showed effects of cytotoxicity with significant increase in Caspase-3 expression in the treated group compared to the control. The potential blood vessel formation induced by VEGF was significantly lower in the cancer cells treated with ACE. Also, MMP2 were highly expressed. We concluded that the Aquilaria crassna extract showed anticancerous effects in human mammay gland cells.
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