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Research Paper | January 1, 2013

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In vitro antimicrobial activity of Lactobacillus isolates against shrimp (Penaeus monodon) pathogens

C. N. Ariole, G. E. Nyeche

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Int. J. Biosci.3(1), 7-12, January 2013

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Abstract

Four isolates of Lactobacillus spp. from the gut of a healthy shrimp (Penaeus monodon) were screened for antimicrobial activity against seven shrimp pathogens (Vibrio spp., Aeromonas spp, Escherichia coli, Staphylococcus sp. and Salmonella sp.) by agar well diffusion assay. Two isolates were found active against all the strains. The challenging experiment showed that Lactobacillus L2 at 1.0 x 106 cfu/ml was enough to suppress Vibrio sp. V2 within 12 hours. The isolated strains Lactobacillus L1 and L2 could have potential against Vibrio sp. V2 under in vitro condition and could be employed as potential probiotics in shrimp aquaculture system for control of bacterial infections.

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In vitro antimicrobial activity of Lactobacillus isolates against shrimp (Penaeus monodon) pathogens

Ajitha S,    Sridhar M,    Sridhar N,    Singh ISB, Varghese V. 2004. Probiotic effects of lactic Acid bacteria against Vibrio alginolyticus in Penaeus(Fenneropenaeus) indicus (H. Milne Edwards). Asian Fisheries Science 17, 71-80.

Ausin B, Stuckey LF, Berton PAW, Effendi I, Griffith DRW. 1995. A probiotic strain of Vibrio alginolyticus effective in reducing diseases caused by Aeromonas salmonicida, Vibrio anguillarum and Vibrio ordalii. Journal of Fish Diseases 18, 93-96.

Baydar NG, Ozkan G, Sagdie O. 2004. Total phenolic contents and antibacterial activities of grape (Vitis vinifera L.) extracts. Food Control 15(5), 335-339.

Chythanya R, Karunasagar I, Karunasagar I. 2002. Inhibition of shrimp pathogenic Vibrios by a marine Pseudomonas 1-2. Aquaculture 208, 1-10.

Dhanasekaran D, Saha S, Thajuddin N, Panneerselvam A. 2008. Probiotic effects of Lactobacillus isolates against bacterial pathogens in Clariasorientalis. Medicine and Biology 15(3), 97-102.

Dobner MJ, Schwaiger S, Jenewein IH, Stuppner H. 2003. Antibacterial activity of Leontopodium alpinum (Edelweiss). Journal of Ethnopharmacology 89, 301-303.

FAO. 2006. State of world aquaculture; 2006 Fisheries Department, Food and Agriculture Organization of the United Nations, Rome, Italy.

Gram L, Melchiorsen J, Spanggaard B, Huber L, Nielsen JF. 1999. Inhibition of Vibrio anguillarum by Pseudomonas fluorescens AH2, a possible probiotic treatment of fish. Applied Environmental Microbiology 65, 969-973.

Hagi T, Tanaka D, Iwamura Y, Hoshino T. 2004. Diversity of seasonal changes in lactic acid bacteria in the intestinal tract of cultured fresh water fish. Aquaculture 234, 335-346.

Holt JG, Krieg NR, Sneath PHA, Stanley JT, Williams ST. (eds). 1994. Bergey’s manual of determinative Bacteriology 9th ed. Williams and Wilkins Baltimore, Maryland, U. S. A.

Itoh T, Fujimoto T, Kawai Y, Toba T, Saito T. 1995. Inhibition of food borne pathogenic bacteria by bacteriocins from Lactobacillus gasseri. Letters in Applied Microbiology 21,137-141.

Joborn A, Olsson JC, Westerdahl A, Conway PL, Kjelleberg S. 1997. Colonisation in the intestinal tract and production of inhibitory substances in intestinal mucus and faecal extracts by Carnobacterium sp. Journal of fish Diseases. 20, 383-392.

Klewicki R, Klewicka E. 2004. Antagonistic activity of lactic acid bacteria as probiotics against selected bacteria of the Enterobacteriacae family in the presence of polyols and their galactosyl derivatives. Biotechnology letters 26, 317-320.

Mombelli B, Gismondo, MR. 2000. The use of probiotics in medicinal practice. International Journal of Antimicrobial Agents 16, 531-536.

Qi Z, Zhang XH, Boon N, Bossier P. 2009. Probiotics in aquaculture of China-Current state, problems and prospect. Aquaculture 290, 15-21.

Rengpipat S, Phianphak W, Piyatiratitivorakul S, Menasveta P. 1998. Effects of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth. Aquaculture 167, 301-313.

Ringo E, Gatesoupe FJ. 1998. Lactic acid bacteria in fish: a review. Aquaculture 160, 177-203.

Singh ISB, Lakshmanaperumalsamy P, Chandramohan. 1998. Bacterial floraof pond reared Penaeus indicus (Milne Edwards). Journal of Aquaculture in the Tropics 13 (2), 133-142.

Torrento M, Torres J. 1996. In vitro inhibitor of Vibrio harveyi by Pseudomonas spp. isolated from aquatic environment. UPV Journal of Natural Science 1, 130-138.

Verschuere L,  Rombaut  G,  Sorgeloos P, Verstraete W. 2000. Probiotic bacteria as biological control agent in aquaculture. Microbiology and Molecular Biology Review 64, 655-671.

Vine NG, Leukes WD, Kaiser H. 2004. In vitro growth characteristics of five candidate aquaculture probiotics and two fish pathogens grown in fish intestinal mucus. FEMS Microbiology Letters 231,145-152.

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