In vitro antioxidant and antiglycation studies on african mistletoe (T. bangwensis (Engler and K. Krause), Danser) and ring worm plant (S. alata (Linn.) Roxb) from Nigeria

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In vitro antioxidant and antiglycation studies on african mistletoe (T. bangwensis (Engler and K. Krause), Danser) and ring worm plant (S. alata (Linn.) Roxb) from Nigeria

Kingsley C.Patrick-Iwuanyanwu, Sajjad Ali, Muhammad Iqbal Choudhary, Muhammad Ismail, Saima Rasheed
J. Bio. Env. Sci.6( 1), 378-386, January 2015.
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The antioxidant activity of crude methanolic extracts and fractions of African mistletoe (Tapinanthus bangwensis) and Ring worm plant (Senna alata) leaves from Nigeria was investigated. In an in vitro free radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide scavenging (SOS) activity and chelation of iron (II) ion [Fe2+] in the presence of ethylenediaminetetraacetic acid (EDTA), ethyl acetate (EtOAc) fractions of both plants showed antioxidant activity in DPPH (83.95±0.04 and 66.35±0.07%), SOS activity (86.10±0.03 and 83.14±0.07%) for T.bangwensis and S.alata respectively compared to control (n-propyl gallate) which showed 90.31±0.01% inhibition). Inhibition of Fe2+ chelation showed 84.29±0.06 and 81.59±0.04% for T.bangwensis and S.alata respectively when compared to control, EDTA (97.60±0.07%). Furthermore, in an advanced glycation end products (AGEs) study, the in vitro inhibition (≥50%) was highest in EtOAc fraction of S.alata (57.06±3.20 and 65.53±1.76%) at 0.25 and 0.5 mg/mL, respectively when compared to the control, rutin (86.00±1.50%). In conclusion, results of the study showed possible antioxidant as well as antiglycation potential of leaves of Tapinanthus bangwensis and Senna alata.


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