In vitro assessment of the prebiotic potential of Caulerpa lentillifera, Gracilaria arcuata, and Sargassum polycystum on probiotic Lactobacillus species
Paper Details
In vitro assessment of the prebiotic potential of Caulerpa lentillifera, Gracilaria arcuata, and Sargassum polycystum on probiotic Lactobacillus species
Abstract
Macroalgae are rich in complex polysaccharides making them a good source of potential prebiotics – non-digestible polysaccharides that promote the growth of beneficial microorganisms in the gut. This study assessed the prebiotic potential of three common Southeast Asian macroalgal species: Caulerpa lentillifera, Gracilaria arcuata, and Sargassum polycystum, on isolated probiotic bacterial species Lactobacillus casei and Lactobacillus paracasei. The Competitive Growth Assay (CGA) of the two Lactobacillus species against the hospital-isolated Escherichia coli, done in Luria Broth with 2% glucose supplementation, was measured using the drop plate method on selective media. The following bacterial combinations for the CGA were: (1) L. casei + E. coli, (2) L. paracasei + E. coli, and (3) both Lactobacillus species + E. coli. Prebiotic potential was assessed by comparing the ratio of Lactobacillus species to E. coli pre- and post-treatment with macroalgae. Data showed that all three macroalgae exhibited significant prebiotic potential (p<0.05) when compared to no prebiotic (negative control), and their prebiotic potentials were comparable to the prebiotic potential of the commercially available prebiotic inulin (positive control). Furthermore, all macroalgae exhibited a significantly stronger prebiotic potential (p<0.05) on L. casei compared to L. paracasei. It is recommended that these macroalgae be part of the regular diet together with the probiotic L. casei. Furthermore, in vivo studies are encouraged to confirm if these macroalgae continue to exhibit their prebiotic effect.
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