Welcome to International Network for Natural Sciences | INNSpub

In vitro assessment of the prebiotic potential of Caulerpa lentillifera, Gracilaria arcuata, and Sargassum polycystum on probiotic Lactobacillus species

Research Paper | May 1, 2017

| Download 12

Kho MJN, Hernandez RBB, Ladera JPT, Khow DAT, Lagura VAM, Jacinto JAC, Medina PMB

Key Words:

Int. J. Biosci.10( 5), 382-388, May 2017

DOI: http://dx.doi.org/10.12692/ijb/10.5.382-388


IJB 2017 [Generate Certificate]


Macroalgae are rich in complex polysaccharides making them a good source of potential prebiotics – non-digestible polysaccharides that promote the growth of beneficial microorganisms in the gut. This study assessed the prebiotic potential of three common Southeast Asian macroalgal species: Caulerpa lentillifera, Gracilaria arcuata, and Sargassum polycystum, on isolated probiotic bacterial species Lactobacillus casei and Lactobacillus paracasei. The Competitive Growth Assay (CGA) of the two Lactobacillus species against the hospital-isolated Escherichia coli, done in Luria Broth with 2% glucose supplementation, was measured using the drop plate method on selective media. The following bacterial combinations for the CGA were: (1) L. casei + E. coli, (2) L. paracasei + E. coli, and (3) both Lactobacillus species + E. coli. Prebiotic potential was assessed by comparing the ratio of Lactobacillus species to E. coli pre- and post-treatment with macroalgae. Data showed that all three macroalgae exhibited significant prebiotic potential (p<0.05) when compared to no prebiotic (negative control), and their prebiotic potentials were comparable to the prebiotic potential of the commercially available prebiotic inulin (positive control). Furthermore, all macroalgae exhibited a significantly stronger prebiotic potential (p<0.05) on L. casei compared to L. paracasei. It is recommended that these macroalgae be part of the regular diet together with the probiotic L. casei. Furthermore, in vivo studies are encouraged to confirm if these macroalgae continue to exhibit their prebiotic effect.


Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

In vitro assessment of the prebiotic potential of Caulerpa lentillifera, Gracilaria arcuata, and Sargassum polycystum on probiotic Lactobacillus species

Bureau of Fisheries and Aquatic Resources. 2010. Fisheries Commodity Roadmap: Seaweeds. Available from www.bfar.da.gov.ph/files/img /photos/roadmapseaweeds_wdcorrction2008.pdf. Accessed Nov. 13, 2016,

Bureau of Fisheries and Aquatic Resources. 2014. Philippine Fisheries Profile 2014. Available from http://www.bfar.da.gov.ph/files/img/photos /2014. Fisheries Profile (Final copy). pdf. Accessed Nov. 13, 2016.

Capra ML, Tibaldo MM, Vinderola G, Reinheimer JA, Quiberoni A. 2014. Technological and probiotic characterisation of Lactobacillus casei/paracasei strains and their phage-resistant mutants. Int. Dairy J 37, 39-47.

Critchley AT. 1993. Gracilaria (Rhodophyta, Gracilariales): an economically important agarophyte, pp. 89-112. In Ohno, M, Critchley AT (Eds.), Seaweed Cultivation and Marine Ranching, 1st Ed. Kanagawa Int. Fisheries Training Center and JICA, Nagai, Japan.

Espitia PJP, Batista RA, Azeredo HMC, Otoni CG. 2016. Probiotics and their potential applications in active edible films and coatings. Food Res. Int 90, 42-52.

Ganzon-Fortes ET. 2012. A historical account of biodiversity studies on Philippine seaweeds (1800-1999). Coast Mar Sci 35(1), 182-201.

Hardy H, Harris J, Lyon E, Beal J, Foey A. 2013. Probiotics, Prebiotics and Immunomodulation of Gut Mucosal Defences: Homeostasis and Immunopathology. Nutrients 5(6), 1869–912.

Hu B, Gong QN, Wang Y, Ma Y, Li J, Yu W. 2006. Prebiotic effects of neoagaro-oligosaccharides prepared by enzymatic hydrolysis of agarose. Anaerobe 12, 260-266.

Kuda T, Ikemori T. 2009. Minerals, polysaccharides and antioxidant properties of aqueous solutions obtained from macroalgal beach-casts in the No to Peninsula, Ishikawa, Japan. Food Chem 12(3), 575–81.

Laparra JM, Velez D, Montoro R, Barberá R, Farré R. 2003. Estimation of arsenic bioaccessibility in edible seaweed by an in vitro digestion method. J. Agric. Food Chem 51(20), 6080–5.

Lynch MB, Sweeney T, Callan JJ, O’Sullivan, JT, O’Doherty JV. 2010. The effect of dietary Laminaria derived laminarin and fucoidan on intestinal microflora and volatile fatty acid concentration in pigs. Livest Sci 133, 157-160.

Murphya P, Bello FD, O’Doherty J, Arendt EK, Sweeney T, Coffey A. 2013. The effect of liquid versus spray-dried Laminaria digitataex tract on selected bacterial groups in the piglet gastrointestinal tract (GIT) microbiota. Anaerobe 21, 1-8.

Nagpal R, Kaur A. 2011. Synbiotic Effect of Various Prebiotics on In Vitro Activities of Probiotic Lactobacilli. Ecol Food Nutr 50(1), 63–8.

Ortiz A, Trono Jr. G. 2007. Growth and Reproductive Pattern of Intertidal and Subtidal Sargassum (Sargassaceae, Phaeophyta) Populations in Bolinao, Pangasinan. Sci Diliman 12(2), 45-50.

Pulz O, Gross W. 2004. Valuable products from biotechnology of microalgae. Appl. Microbiol. Biotechnol 65(6), 635–48.

Slavin J. 2013. Fiber and Prebiotics: Mechanisms and Health Benefits. Nutrients 5(4), 1417–1435.

Sweeney T, Dillon S, Fanning J, Egan J, O’Shea CJ, Figat S, Gutierrez JJM, Mannion C, Leonard F, O’Doherty JV. 2011. Evaluation of seaweed-derived polysaccharides on indices of gastrointestinal fermentation and selected populations of microbiota in newly weaned pigs challenged with Salmonella typhimurium. Anim. Feed Sci. Technol 165, 85-94.

Wang Y, Han F, Hu B, Li JB, Yu WG. 2006. In vivo prebiotic properties of alginate oligosaccharides prepared through enzymatic hydrolysis of alginate. Nutr Res 26, 597-603.

Zaporozhets TS, Besednova NN, Kuznetsova TA, Zvyagintseva TN, Makarenkova ID, Kryzhanovsky SP, Melnikov VG. 2014. The prebiotic potential of polysaccharides and extracts of seaweeds. Russ J Mar Biol 40(1), 1–9.