Paper Details

In vitro culture and callus induction of chamomile (Matricaria chamomilla L.) explants under different concentrations of plant growth regulators

Vahid Sayadi, Ali Ashraf Mehrabi, Mehdi Saidi, Khoshnood Nourollahi

DOI: https://dx.doi.org/10.12692/ijb/4.10.206-211

Int. J. Biosci. 4(10), 206-211. May, 2014. (PDF)

Abstract:

Chamomile (Matricaria chamomilla L.) is one of the most important herbal medicine plants. The aim of this research was to study effect of different concentrations of growth regulators of Auxin on callus induction and growth in Chamomile’s various explants; factors were: NAA at four levels (0, 1, 2, 4 mgl-1) Kinetin at four levels (0, 0.5, 1, 2 mgl-1), explants cultured on basal MS medium containing growth regulators. We found that callus initiation in explants was faster at 1 mgl-1 NAA and 1 mgl-1 Kinetin. Leaf explants on a medium containing 1 mgl-1 NAA and 1 mgl-1 Kinetin has been highest percentage of callus induction with an average of 93.26 percent. Axillary bud and stem discs explants have been the highest percentage of callus induction with an average of 89.68 and 80.75 %, respectively, is obtained in same treatment combination. The effect of different concentrations of NAA and Kinetin on callus volume that derived from different explants is significant and stem explants in 1 mgl-1 NAA and 1 mgl-1 Kinetin have been produced maximum callus volume with an average 17.49.