In-vitro Cytotoxicity of Wrightia pubescens (Blanco) Merr., Aphanamixis polystachya (Wall.) Parker, and Platymitra arborea (Blanco) against selected human cancer cell lines

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In-vitro Cytotoxicity of Wrightia pubescens (Blanco) Merr., Aphanamixis polystachya (Wall.) Parker, and Platymitra arborea (Blanco) against selected human cancer cell lines

Maria Lorraine Garcia Bugayong , Sonia Donaldo Jacinto
Int. J. Biosci.11( 5), 204-213, November 2017.
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Cancer is the second leading cause of mortality worldwide. Anticancer studies are centered on natural products as these have been found to exhibit properties that activate cell signaling pathways and cell aging and senescence. As such, there is a need for continuing research to explore these natural products especially those that do not only exhibit cytotoxicity but also specificity against cancer cells. This study evaluated the cytotoxic activity of the crude extracts of three Philippine indigenous plants, Wrightia pubescens (Blanco) Merr. Aphanamixis polystachya (Wall.) Parker, and Platymitra arborea (Blanco) against selected human cancer cell lines using 3-(4,5- dimethylthiazol-2-yl)-2-5-diphenyl-2H-tetrazolium bromide (MTT) assay. Extracts were partitioned using hexane and ethyl acetate to determine their active fractions based on their polarity. These active fractions were then tested for their cytotoxicity against human colorectal cancer cell line (HCT116), human adenocarcinoma cell line (A549) and non-cancer Chinese hamster ovary cell line (AA8).  Cytotoxic activities of the extracts were found in the ethyl acetate fractions of W. pubescens and P. arborea and hexane fraction of A. polystachya. All active fractions were highly cytotoxic to HCT116 and A549, with A. polystachya exhibiting the highest selectivity against cancer over the non-cancer cells. Results of this study imply that these extracts, especially that of A. polystachya, have a potential use in anti-cancer research due to their selectivity against cancer cell lines.


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