In-vitro effect of domesticated animal’s saliva against puss forming bacteria

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Research Paper 01/04/2019
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In-vitro effect of domesticated animal’s saliva against puss forming bacteria

Muhammad Muzammal, Saqib Ali Rustam, Shawana Huma, Muhammad Sohaib, Safeer Ahmad, Muhammad Zeeshan Ali, Kaleem Ullah shah, Muhammad Hanif, Rizwan, Fatima, Sohail Ahmad, Saima Mashal, Hafsa Qureshi. Safeena Sadiq
Int. J. Biosci.14( 4), 392-398, April 2019.
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Puss forming bacteria increasing their resistance against number of antibiotics, this study was done to check the antibacterial activity of domesticated animal’s saliva before and after feeding against 5 different puss forming bacteria using disc diffusion method. In this study saliva was taken from three domesticated animals i.e. dog, horse and cat and examine against five puss forming bacteria i.e. Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Salmonella typhi and Escherichia coli. Among the test saliva, highest antibacterial activity was shown by dog saliva (21+1mm before feeding &23+1mm after feeding)against Escherichia coli and its activity was lowest(16+1mmbefore feeding and19+1mm after feeding) against Staphylococcus epidermidis. In case of cat, its saliva shown highest activity (20+0.6mm before feeding &22+0.8mm after feeding) against Staphylococcus aureus and its activity was lowest (16+0.6mm before feeding &16.5+0.9mm after feeding) against Streptococcus pyogenes and in case of horse highest antibacterial activity (18+0.3mm before feeding and 18.2+0.7mm after feeding) was shown by its saliva against Salmonella typhi and lowest (16+1 mm before feeding) against Staphylococcus aureus and after feeding its activity was lowest (17.6+0.5mm) against Staphylococcus epidermidis. Before and after feeding the pH of saliva was also measured. PH of dog saliva before and after feeding was 8.46 and 8.22, for cat it were 8.1 and 7.9 and for horse it were 7.2 and 6.5 respectively. Form the result it has been suggested that animal’s saliva has high antibacterial activity against number of pathogenic bacteria and can be used as antibacterial drug in near future.


Davis SJ, Valla FR. 1978. Evidence for domestication of the dog 12,000 years ago in the Natufian of Israel. Nature 276(5688), 608.

De Almeida PDV, Gregio AM, Machado MA, De Lima AA, Azevedo LR. 2008. Saliva composition and functions: a comprehensive review. The journal of contemporary Dental Practice 9(3), 72-80.

Hart BL, Korinek E, Brennan P. 1987.Postcopulatory genital grooming in male rats: Prevention of sexually transmitted infections. physiology and behavior. 41, 321-325.

Ku BK, Jeon BY, Kim JM, Jang YB, Lee H, Choi JY, Cho SN. 2018. Investigation of bovine tuberculosis outbreaks by using a trace-back system and molecular typing in Korean Hanwoo beef cattle. Journal of veterinary science 19(1), 45-50.

Larson G, Fuller DQ. 2014. The evolution of animal domestication. Annual Review of Ecology, Evolution, and Systematics 45, 115-136.

Mandel ID, Ellison SA. 1981. Naturally occurring defense mechanisms in saliva. In: Tanzer, J. M., ed. Animal models in cardiology: Proc. symp. Workshop in cardiology. Washington, DC: Information Retrieval 367-379.

Omenka CA, Osuoha JO. 2000. Antimicrobial potency of Grapefruit seed extract on five selected pathogens. Nigerian Journal of Microbiology 14(2), 39-42.

Pickering CM, Hill W, Newsome D, Leung YF. 2010. Comparing hiking, mountain biking and horse riding impacts on vegetation and soils in Australia and the United States of America. Journal of environmental management, 91(3), 551-562.

Pollock JJ, Denepitiya L, Mackay BJ, Iacono VJ. 1984. Fungi- static and fungicidal activity of the human parotid salivary histidine- rich polypeptides on Candida albicans.Infection and immunity. 44, 702-707.

Roth L, Rosenblatt JS. 1967.Changes in self-licking during pregnancy in the rat. Journal of Comparative and Physiological Psychology. 63, 397400.

Schneirla TC, Rosenblatt JS. 1963.Tobach, E. Maternal behavior of the cat. Maternal behavior of mammals. New York: John Wiley and Sons. 122-168.

Thompson R. 1940. Lysozyme and its relation to the antibacterial properties of various tissues and secretions Archives of Pathology & Laboratory Medicine Online 30, 1096-1134.

Wang, Xiaoming, Tedford, Richard H. 2008. Dogs: Their Fossil Relatives and Evolutionary History. New York: Columbia University Press, page 1.

Zeder M. 2012. The domestication of animals. Journal of Anthropological Research, 68(2), 161-190.