In-vitro effect of domesticated animal’s saliva against puss forming bacteria

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Research Paper 01/04/2019
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In-vitro effect of domesticated animal’s saliva against puss forming bacteria

Muhammad Muzammal, Saqib Ali Rustam, Shawana Huma, Muhammad Sohaib, Safeer Ahmad, Muhammad Zeeshan Ali, Kaleem Ullah shah, Muhammad Hanif, Rizwan, Fatima, Sohail Ahmad, Saima Mashal, Hafsa Qureshi. Safeena Sadiq
Int. J. Biosci.14( 4), 392-398, April 2019.
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Abstract

Puss forming bacteria increasing their resistance against number of antibiotics, this study was done to check the antibacterial activity of domesticated animal’s saliva before and after feeding against 5 different puss forming bacteria using disc diffusion method. In this study saliva was taken from three domesticated animals i.e. dog, horse and cat and examine against five puss forming bacteria i.e. Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Salmonella typhi and Escherichia coli. Among the test saliva, highest antibacterial activity was shown by dog saliva (21+1mm before feeding &23+1mm after feeding)against Escherichia coli and its activity was lowest(16+1mmbefore feeding and19+1mm after feeding) against Staphylococcus epidermidis. In case of cat, its saliva shown highest activity (20+0.6mm before feeding &22+0.8mm after feeding) against Staphylococcus aureus and its activity was lowest (16+0.6mm before feeding &16.5+0.9mm after feeding) against Streptococcus pyogenes and in case of horse highest antibacterial activity (18+0.3mm before feeding and 18.2+0.7mm after feeding) was shown by its saliva against Salmonella typhi and lowest (16+1 mm before feeding) against Staphylococcus aureus and after feeding its activity was lowest (17.6+0.5mm) against Staphylococcus epidermidis. Before and after feeding the pH of saliva was also measured. PH of dog saliva before and after feeding was 8.46 and 8.22, for cat it were 8.1 and 7.9 and for horse it were 7.2 and 6.5 respectively. Form the result it has been suggested that animal’s saliva has high antibacterial activity against number of pathogenic bacteria and can be used as antibacterial drug in near future.

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