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In vitro evaluation of antibacterial and antifungal activates of Iphiona aucheri leaves extracts

Research Paper | September 1, 2019

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Muhammad Abdur Rehman Shah, Rahmat Ali Khan, Mushtaq Ahmed, Fazal Dian, Manzoor Ullah

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Int. J. Biosci.15( 3), 175-182, September 2019

DOI: http://dx.doi.org/10.12692/ijb/15.3.175-182


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The aim of the current project was to evaluate the antibacterial and antifungal activities of Iphiona aucheri leaves methanolic extract and its n-hexane, chloroform and aqueous fractions. Fine powder of Iphiona aucheri leaves were extracted in 70% methanol and then subjected to sequential fractionation through n-hexane, chloroform and water. All the fractions were assessed for their antimicrobial characteristics using agar well diffusion assay. The results revealed that the applied extracts have significant antimicrobial characteristics at 30mg/ml concentration on gram positive bacteria (Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes) and four gram negative bacteria (Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae, Pseudomonas aeruginosa) and two fungal strains, Aspergillus flavus and Aspergillus niger. The Aspergillus fumigates was found resistant to the applied samples. The maximum inhibition of bacterial growth caused by methanolic extract and its chloroform and aqueous fractions was found (13.7±0.57), (15.7±1.52) and (9.87±1.46) respectively against S. aureus while n-hexane expressed maximum inhibition (5.2±0.57) against K. pneumonia growth. The growth of A. niger was highly inhibited by chloroform fraction (47.43±1.27%), followed by methanolic extract (42.15±1.46%), aqueous fraction (34.45±0.52%) and n-hexane fraction (21.22±1.15%). The chloroform fraction with MIC values (1.6±0.35 mg/ml) and (160.75 ±0.57 µg/ml) against S. aureus and A. niger respectively was found most effective. In conclusion, the result of our study indicated that the leaves of Iphiona aucheri possess considerable antimicrobial activities and thus will be of great use in developing plant derived antimicrobial and chemotherapeutic agents.


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In vitro evaluation of antibacterial and antifungal activates of Iphiona aucheri leaves extracts

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