In vitro evaluation of the molluscicidal activity of Euphorbia tirucalli latex extract against the mollusk rice pest Pomacea canaliculata (Caenogastropoda: Ampullariidae)

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Research Paper 01/08/2018
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In vitro evaluation of the molluscicidal activity of Euphorbia tirucalli latex extract against the mollusk rice pest Pomacea canaliculata (Caenogastropoda: Ampullariidae)

Kevin Johns C. Edis, Florevil S. Basay, Van Lindzie T. Castillo, Dinkey Ryalle Alegado, Adelaine Alicante, Jessa May Alon, Rachelle Garcia, Fijie Gepiga, Jay P. Picardal
J. Bio. Env. Sci.13( 2), 237-245, August 2018.
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The latex of Euphorbia tirucalli (“Pobreng Kahoy”) is widely known for its medicinal, antimicrobial, and insecticidal properties. Observations on the plant’s molluscicidal potency were reported by local farmers. In this study, the molluscicidal activity of aqueous latex extract (ALE) of E. tirucalli was evaluated against adult Golden apple snail (“Kuhol”, Pomacea canaliculata), a rice seedling pest. This research employed a Completely Randomized Design in triplicates (3 trials, N=450) under 3 experimental treatments (T1= 8.75 ppm, T2= 7.5 ppm ALE, T3= 6.25 ppm ALE) and two control groups (Positive Control=Niclosamide [Snail ShatterTM] and Negative Control= distilled H2O [AbsoluteTM]). After 96-hours experimental period, mortality data across treatment groups were analyzed using Probit analysis, One Way ANOVA (p<0.05) followed by Tukey’s HSD test. Results revealed that there is a positive correlation between ALE concentration and snail mortality and T1 (8.75 ppm) was reported to have a comparable lethal effect to that of the commercially-available molluscicide (Niclosamide). Lethal dosages of the aqueous extract of the E. tirucalli latex was LD50=6.33 ppm ALE and LD90=9.36 ppm ALE. For future research, it is recommended to have phytochemical screening of similar ALE concentrations and toxicity level assessment to economically important non-target organisms (Tilapia Oreochromis niloticus and Water fleas Daphnia spp.) under field trials.


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