Paper Details

In vitro growth responses of two citrus species, i.e. Pummelo (Citrus grandis L.) and Key lime (Citrus aurantifolia Cristm.) in various sucrose concentrations

Shefali Khatun, Farhana Akter, S.M. Khaledur Rahman, A. M. Swaraz

DOI: https://dx.doi.org/10.12692/ijb/5.12.518-524

Int. J. Biosci. 5(12), 518-524. December, 2014. (PDF)

Abstract:

Studies were performed to investigate in vitro growth responses of seeds of two citrus species, viz. Pummelo and Key lime in various sucrose concentrations in an attempt to generate cost efficient in vitro citrus plant. Mature seeds collected from ripe fruits and after surface sterilization were cultured on different sucrose concentration containing MS media and compared them with control.The results were scrutinized by different parameters namely on seed swelling, radical and first root emergence, first and second pair leaf emergence. In seed swelling experiment, 2.50 % sucrose containing media for Pummelo and sucrose free medium for Key lime showed best performance. Radical emergence and first root initiation was observed best in 2.50 % sucrose containing MS media in both citrus species. First pair of leaf emergence was better in MS0 and 2.50 % of sucrose containing media in Pummelo whereas 1.0 %, 2.0 % and 2.50 % sucrose concentration showed better result in Key lime. In case of second pair leaf proliferation there were no impact of sucrose concentration on leaf  formation but fast response depend on sucrose concentration and 2.50 % sucrose containing media showed best response in both citrus. Root induction from in vitro grown shoots-cutting was responded quickest  in 1.0 % sucrose containing media in both citrus. Plantlets of both citrus species generated through in vitro culture were able to acclimatize successfully and confirmed natural growth on soil when transferred them in earthen pots.