In vitro organogenesis of Ageratum houstonianum (Asteraceae)

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Research Paper 01/05/2017
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In vitro organogenesis of Ageratum houstonianum (Asteraceae)

Mastaneh Mohammadi
Int. J. Biosci.10( 5), 85-96, May 2017.
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Regeneration of whole plants from tissue explants is a key technology for crop improvement and plant biotechnology and has critically contributed to the green revolution. In this study, the effects of MS medium supplemented with various concentrations of Kin and IAA combination on induction of callus, shoot and root induction and multiplication, and plantlet formation in various explants of Ageratum houstonianum were investigated. Maximum number of callus formation was observed in leaf at 1-1 and 4-4 (mg L−1), and in shoot apical meristem at 2-2 (mg L−1) of Kin and IAA combination. 0.4-1 (mg L−1) was the best combination for root and shoot regeneration on root and leaf explants, respectively. The maximum number of both root and shoot formation in epicotyl explant was observed at 2-4 (mg L−1). Among the explants, shoot apical meristems were responding for regeneration of plantlets, and the optimum concentration of Kin-IAA to produce plantlet was 4-2 (mg L−1) combination. This study would be an important step towards the development of good quality of organogenesis to provide successful plantlet production from other Ageratum species.


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