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Research Paper | February 1, 2011

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In vitro plant regeneration from male flowers of banana

M Tipu Sultan, Mahboob Hossain Khan, M Lokman Hakim, ANK Mamun, M Alam Morshed, M Rofiqul Islam, M Rokibul Islam

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Int. J. Biosci.1(1), 1-11, February 2011


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The present study was undertaken with a view to establish a protocol on in vitro regeneration of plants by using young male flowers of banana (Musa sp. cv. Sabri) as explant. Appropriate developmental stage of immature male flowers for inoculation, medium composition for induction of calli, regeneration of plants, rooting of in vitro regenerated shoots, acclimatization of in vitro regenerated plantlets and ex vitro establishments of plantlets were worked out. Young male flowers obtained by striping away the bracts in between 24 to 26 were found suitable as explants for induction of callus. The isolated male flowers were cultured on MS (Murashige and Skoog) medium supplemented with different concentrations and combinations of three auxins viz. 2, 4-D (2,4-Dichlorophenoxyacetic acid), NAA (Napthaleneacetic acid) and IAA (Indole-3-acetic acid) for induction of callus. Only two of the two medium composition yielded calli. The better response (20%) was recorded in MS medium containing 2.0 2, 4-D + 0.5 NAA + 0.5 IAA (Indole-3-acetic acid) (mg/l). The calli were cultured on MS medium fortified with different concentrations of BA (Benzyladenine), NAA, IAA and Glutamine or Caesin hydrolysate (CH) to regenerate shoots. MS medium having the supplementation of 1.0 BA + 0.5 IAA + 500 CH (mg/l) was appeared best for regeneration of shoots. Single isolated regenerated shoots were implanted on MS medium supplemented with three different concentrations (0.5, 1.0 and 2.0 mg/l) of IBA or NAA to induce root. IBA at a concentration of 1.0 mg/l produced best rooting. The plantlets were gradually acclimatized and transferred to the soil. The survival percentage was about 90%.


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In vitro plant regeneration from male flowers of banana

Kabir AH, Mahfuz I, Razvy MA, M. MB, Alam MF. 2008. Indirect Organogenesis and Somaclonal Variation in Four Rice Cultivars of Bangladesh. Journal of Applied Sciences Research 4(4), 451-458.

Alsadon AA, Al-Mohaideb M, Rahman MH, Islam R. 2004. Evaluation of vegetative growth traits of eight potato cultivars. Bangladesh J. Genet. Biotech. 5(1&2), 61-64.

Assani A, Bakry F., Kerbellec F. 2001. Plant regeneration from protoplasts of dessert banana Grande Naine (Musa spp., Cavendish sub-group AAA) via somatic embryogenesis, Plant Cell Rep. 20, 482.

Bakry F, Assani A, Kerbellec F. 2008. Haploid Induction: androgenesis in Musa balbisiana. Fruits 63, 45-49.

Bhaskar J, Arvindakchan M, Balsalakumari PK, Rajeevan PK. 1993. Micropropagation studies in banana. South Indian Hort. 41, 186-191.

Denise M. Seliskar, John L. Gallagher. 2000. Exploiting wild population diversity and somaclonal variation in the salt marsh grass Distichlis spicata (Poaceae) for marsh creation and restoration; American Journal of Botany 87(1), 141–146.

Hakim L, Ahmed G, Kabir H, Mamun ANK, Roy PK, Islam MR, Khan R. 2008. Production of doubled haploids in banana. Abstract. Proc. FAO/IAEA Intl. Symp. Induced Mutations in Plants held in Vienna, Austria from 12-15 Aug., 2008. IAEA-CN-167-263P, 121.

Hong W, Debergh P. 1995. Somatic embryogenesis and plant regeneration in garden leek. Plant Cell Tissue and Organ Culture 43, 21-28.

Islam S, Hoque A. 2004. Status of banana production in Bangladesh. Molina, A.B., Eusebio, J.E., V.N. Roa, I. Van Den Bergh, M.A.G. Maghuyop, K.H. Borromeo (eds.). Advancing banana and plantain R&D in Asia and the Pacific, Proc. 2nd BAPNET Steering Committee Meeting, Jakarta, Indonesia 12, 33-41.

Kaeppler SM, Kaeppler H.F, Rhee Y. 2000. Epigenetics aspect of somaclonal variation in plants. Plant Mol. Biol. 43, 179-188.

Khanam D, Hoque MA, Khan M.A., Quasem A. 1996. In vitro propagation of banana (Musa sp.). Plant Tissue Culture 6, 89-94.

Kulkarni VM, Suprasanna P, Bapat VA. 2006. Plant regeneration through multiple shoot formation and somatic embryogenesis in a commercially important and endangered Indian banana cv. Rajeli. Curr. Sci. 90(6), 842-850.

Larkin PJ, Scowcroft WR. 1981. Somaclonal variation and crop improvement. In:Genetic Engineering of Plants. An Agricultural Perspective, T. Kosuge, C.R. Meredilh and M. Hollander (eds.) Plenum Press, 289-314.

Nasrin S, Hossain MM, Khatun A, Alam MF, Mondal MRK. 2003. Induction and evaluation of somaclonal variation in potato (Solanum tuberosum L.). Bio. Sci. 3(2), 183-190.

Novak FJ, Afza R, Duren MV, Omar MS. 1990. Mutation Induction by gamma irradiation of in vitro cultured shoot-tips of banana and plantain (Musa sp.), Trop. Agr. (Trinidad) 67, 21-28.

Raut RS, Lakhnade VE. 1989. Propagation of plantain through meristem culture. Ann. Plant. Physiology 3, 256-260.

Roux N. 2004. Mutation induction in Musa – review. In: Banana Improvement –Cellular, Molecular Biology and Induced Mutations (Mohan J. and S. R. Swennen, Eds.), Science Publishers, Inc., Enfield (NH), USA, Plymouth, UK, 23-32.


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