In vitro propagation of a medicinal plant – mango ginger (Curcuma amada Roxb.)

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Research Paper 01/11/2012
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In vitro propagation of a medicinal plant – mango ginger (Curcuma amada Roxb.)

M. M. Ferdous, M. Shahinozzaman, M. O. Faruq, S. P. Paul, M. A. K. Azad, M. N. Amin
Int. J. Biosci.2( 11), 166-172, November 2012.
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Abstract

This paper describes efficient in vitro propagation protocol of Curcuma amada Roxb., an important aromatic zingiberaceous plant in the countries of Indian subcontinent, using rhizome explants. Rhizome tip explants showed better response than rhizome bud explants in terms of shoot proliferation and hence excised rhizome tip explants were inoculated aseptically on Murashige and Skoog’s (MS) medium supplemented with different 6-Benzyl adenine (BA) or 6-furfurylamino purine (Kn) concentrations (0.0, 2.0, 4.0, 6.0, 8.0, 10.0 µM) alone or in combinations with α- Naphthalene acetic acid (NAA) and Indole-3-butyric acid (IBA) (1.0, 1.5, 2.0 µM). Optimum proliferation was obtained on MS medium having 8.0 µM BA + 1.0 µM NAA. Shoot proliferation was maximal (99.8%) with 10.6 ± 0.26 shoots per explant after 6 weeks of culture. The successfully proliferated shoots with an average height of 38 cm were transferred to rooting medium augmented with NAA and IBA at four different concentrations (2.0, 4.0, 6.0, 8.0 µM) and 4.0 µM IBA was found to be best for in vitro adventitious rooting. Rooted plantlets were then transferred to plastic cups containing autoclaved garden soil and compost (1:1), gradually acclimatized and finally transferred to the field condition with 80% survival rate.

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Ali A, Munawar A, Siddiqui FA. 2004. In vitro propagation of turmeric, Curcuma longa L. Int. J. Biol. Biotechnol. 1, 511-518.

Balachandran  SM,  Bhat  SR,  Chandal  KPS. 1990. In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rosc). Plant Cell Rep. 8, 521- 524.

Balachandran  SM,  Bhat  SR,  Chandal  KPS. 1990. In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rosc.). Plant Cell Rep. 8, 521- 524.

Barthakur MP, Bordoloi DN. 1992. Micropropagation of Curcuma amada Roxb. J. Spice. Arom. Crops 1, 154–156.

Bharalee R, Das A, Kalita MC. 2005. In vitro clonal propagation of Curcuma ceasia Roxb. and Curcuma zedoaria Rosc. from rhizome bud explants. J. Plant Biochem. Biotechnol. 14, 61- 63.

Chan LK, Thong WH. 2004. In vitro propagation of Zingiberaceae species with medicinal properties. J. Plant Biotechnol. 6, 181- 188.

Chougule PS. 2008. Studies on microrhizome production in Turmeric. M.Sc. Thesis, University of agricultural sciences, India, p. 51.

Das A, Kesari V, Rangan L. 2010. Plant regeneration in Curcuma species and assessment of genetic stability of regenerated plants. Biol. Plant. 54, 423- 429.

Dogra SP, Koria BN, Nad Sharma PP. 1994. In vitro clonal propagation of ginger (Zingiber officinale Rosc.). Horticultural Journal 7, 45- 50.

Ghani A. 1998. Medicinal Plants of Bangladesh: Chemical Constituents and use. Asiatic Society, p. 290- 291.

Hoque HI, Perveen S, Sarker RH. 1999. In vitro propagation of ginger (Zingiber officinale Rosc.). Plant Tissue Cult. 9, 45- 51.

Hosoki T, Sagawa Y. 1977. Clonal micropropagation of ginger (Zingiber officinale Rosc.) through tissue culture. J. Hort. Sci. 12, 451-452.

Hussain A, Virmani OP, Popli SP, Misra LN, Gupta MM. 1992. in Dictionary of Indian medicinal plants (Lucknow: CIMAP) p. 161- 162.

Inden H, Hirano A, Asahira T. 1998. Micropropagation of ginger. Acta. Hortic. 230, 177-184.

Islam MA, Kloppstech K, Jacobsen HJ. 2004. Efficient procedure for in vitro microrhizome induction in Curcuma longa L. (Zingiberaceae) – a medicinal plant of tropical asia. Plant Tissue Cult. 14, 123- 134.

Kambaska KB, Debashrita P, Santilata  S. 2010. Effect of plant growth regulator on in vitro multiplication of turmeric (Curcuma longa L. cv.Ranga). International Journal of Biological Technology 1, 16- 23.

Kambaska KB, Santilata S. 2009. Effect of plant growth regulator on micropropagation of ginger (Zingiber officinale Rosc.) cv- Suprava and Suruchi. Journal of Agricultural Technology 5, 271- 280.

Keshavachandaran R, Khader MA. 1989. Tissue culture propagation of turmeric. South Indian Hortic. 37, 101- 102.

Meenakshi N, Sulikeri GS, Krishnamoorthy V, Ramakrishna VH. 2001. Standardization of chemical environment for multiple shoot induction of turmeric (Curcuma longa L.) for in vitro clonal propagation. Crop Research 22. 449- 453.

Mukhri T, Yamaguchi H. 1986. In vitro plant multiplication from rhizomes of turmeric (Curcuma domestica Val.) and Ternoe Lawak (C. xanthorhiza Roxb.). Plant Tiss, Cult. Lett. 3, 28- 30.

Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473- 497.

Nadgauda RS, Mascarenhas AF, Hendre RR, Jagannathan V. 1978. Rapid multiplication of turmeric (Curcuma longa Linn.) plants by tissue culture. Indian J. Exp. Biol. 16, 120- 122.

Nasirujjaman K, Udden MS, Zaman S, Reza MA. 2005. Micropropagation of turmeric (Curcuma longa L.) through in vitro rhizome bud culture. Journal of Biological Sciences 5, 490- 492.

Nayak S. 2000. In vitro multiplication and microrhizome induction in Curcuma aromatica Salisb. Plant Growth Regulation 32, 41- 47.

Noguchi Y, Yamakawa O. 1988. Rapid clonal propagation of ginger (Zingiber officinale Rosc.) by roller tube culture. Japan J. Breed. 38, 437- 442.

Pandey PY, Sagswansupyakom C, Sahavvachadria O, Thaveechae N, Pandey Y. 1997. In vitro propagation of ginger (Zingiber officinale). Kastetsaet Journal of Natural Science 3. 81- 86.

Prain D. 1981. Curcuma amada Roxb. In: Ray PC, ed. Bengal Plants, Vol. II. Sri Gouranga Press Pvt. Ltd. Calcutta, p. 1042.

Prakash S, Elangomathavan R, Seshadri S, Kathiravan K, Ignacimu-thu S. 2004. Efficient regeneration of Curcuma amada Roxb. plantlets from rhizome and leaf sheath explants. Plant. Cell. Tiss. Org. Cult. 78, 159- 165.

Prakash S, Elangomathavan R, Seshadri S, Kathiravan K, Ignacimuthu S. 2004. Efficient regeneration of Curcuma amada Roxb. Plantlets from rhizome and leaf sheath explants. Plant. Cell. Tiss. Org. Cult. 78, 159- 165.

Rahman MM, Amin MN, Jahan HS, Ahmed R. 2004. In vitro regeneration of plantlets of Curcuma longa L. a volume spice plant of Bangladesh. Asian Journal of Plant Sciences 3, 306- 609.

Salvi ND, George L, Eapen S. 2000. Direct regeneration of shoots from immature inflorescence cultures of turmeric. Plant Cell Tiss. Org. Cult. 62, 235- 238.

Salvi ND, George L, Eapen S. 2001. Plant regeneration from leaf base callus of turmeric and random amplified polymorphic DNA analysis of regenerated plants. Plant Cell Tiss. Org. Cult. 66, 113- 119.

Salvi ND, George L, Eapen S. 2002. Micropropagation and field evaluation of micropropagated plants of turmeric. Plant. Cell. Tiss. Org. Cult. 68, 143- 151.

Shankaracharya NB. 1982. Mango ginger. Indian Cocoa. Arecanut Spices J. 5, 78–80.

Sit AK, Tiwari RS. 1997. Micropropagation of turmeric (Curcuma longa L.). Rec. Hortic. 4, 145-148.

Srivastava AK, Srivastava SK, Shah NC. 2001. Constituents of the rhizome essential oil of Curcuma amada Roxb. from India. J. Ess. Oil Res. 13, 63- 64.

Stanly C, Keng CL. 2007. Micropropagation of Curcuma zedoaria Roscoe and Zingiber zerumbet Smith. Biotechnology 6, 555-560.

Sunitibala H, Damayanti M, Sharma GJ. 2001. In vitro propagation and rhizome formation in Curcuma longa Linn. Cytobios 105. 71- 82.

Verghese J. 1990. Mango ginger – an exotic flavourant. Indian Spices 27, 15–16.