In vitro regeneration of niger (Guizotia abyssinica L.F.) Cass.)

Paper Details

Research Paper 01/12/2011
Views (299) Download (4)

In vitro regeneration of niger (Guizotia abyssinica L.F.) Cass.)

Tesfaye Disasa, Tileye Feyissa, Kefle Dagne
Int. J. Biosci.1( 6), 110-118, December 2011.
Certificate: IJB 2011 [Generate Certificate]


Callus induction and shoot formation from hypocotyls and cotyledons of G. abyssinica has been achieved in this experiment. Six to eight-day-old hypocotyl segments and cotyledons were cultured on MS medium containing different concentrations of NAA, IAA and BAP. Among the various concentrations tested, 0.5 mg/l NAA in combination with 1 mg/l BAP was found to be the best for maximum callus induction of hypocotyl explants. Furthermore, 2 mg/l IAA in combination with 1 mg/l BAP was the best for callus induction of cotyledonary explants. The frequency of callus induction was influenced by the concentrations and types of growth regulators. Highest percentage of shoot formation was obtained when cotyledons were cultured on medium supplemented with 3.0 mg/l IAA in combination with 1.0 mg/ l BAP. Maximum number of shoots per explant (20.3) was obtained from medium containing 0.1 mg/l NAA in combination with 1 mg/l BAP. The types of explant, growth regulator combinations and genotypes were showed significant effect on shoot regeneration. The elongated shoots were successfully rooted on media supplemented with IBA at a concentration of 0.5 mg/l. The shoots were established in soil where 65% of them survived. Morphologically aberrant plants were not observed. As plant regeneration protocol is a prerequisite for genetic transformations, this protocol can be used for such purposes and development of new varieties with desired traits by in vitro selection.


Baagǿe J. 1974. The genus Guizotia (Compositeae). A taxonomic revision. Bot. Tidskr. 69, 1-39.

Babbar SB, Kaur A, Walia N. 2005. In vitro regeneration of a high oil-yielding variety of safflower (Carthamus tinctorius var HUS-305). J. Plant Biochem. and Biotechnol. 14(1), 65-68.

Belayneh H.1986. Source –Sink study on niger. Oil Crops Newsl 3, 63-65.

Belayneh H. 1991. Oil crop germplasm: a vital resource for a plant breeder. In: Engels JM, Hawkes JG, Worede M, ed. Plant genetic resources of Ethiopia. Cambridge, UK: Cambridge University Press, 344-354

Dagne K. 1994. Cytology, Phylogeny and Oil quality of Guizotia Cass. (Compositeae). Ph.D. Dissertation, Addis Ababa University, Ethiopia.

Duke JA. 1983. Guizotia abyssinica Cass. Niger seed. Handbook of energy crops (unpublished).

Gurel E, Kazan K. 1998. Development of an Efficient Plant Regeneration System in Sunflower (Helianthus annuus L.). Tr.J. Bot. 22, 381-387.

Khehra GS, Mathias RJ. 1992. The interaction of genotype, explant and media on the regeneration of shoots from explants of Brassica napus L. J. Exp. Bot. 43, 1413-1418.

McCown BH. 1986. Wood ornamentals, shade trees and conifers In: Zimmerman RH, Griesbach RJ, Hammerschlay FA, Lawson RH, ed. Tissue culture as a plant production system for horticultural crops. Dordrecht, Germany: Martinus Nijhoff Publishers,333-342.

Murashige T, Skoog F. 1962. A revised medium for rapid growth and biossays with tobacco tissue cultures. Physol. Plant. 15, 473-497.

Murthy HN, Jeong JH, Choi YE, Paek KY. 2003. Agrobacterium-mediated transformation of niger [ Guizotia abyssinica (L. f.) Cass.] using seedling explants. Plant Cell Reports 21 (12), 1183-1187.

Nikam TD, Shitole MG. 1993. Regeneration of niger (Guizotia abyssinica Cass.) cv. Sahyadri from seedling explants. Plant Cell Tiss. Organ. Cult. 32, 345-349.

Ohki S, Nasuda K, Katsuta H. 1991. In vitro nursery system for vegetable crops-tomato and melon. In: Bajaj YPS, ed. Biotechnology in agriculture and forestry: High-tech and micro propagation. Berlin, Germany: Heidelberg press, 344-358.

Qin Y, Gao LH, Pulli S, Guo YD. 2006. Shoot differentiation, regeneration of cauliflower and analysis of somaclonal Variation by RAPD. Hereditas 143, 91-98.

Riley KW, Belayneh H. 1989. Niger. In: Robbenelen G, Downey RK, Ashari A. ed. Oil Crops of the World: their breeding and utilization. New York, USA: McGraw-hill, 394-403.

Sarvesh A, Reddy TP, KaviKishor PB. 1993. Plant regeneration from cotyledon of niger. Plant Cell Tiss. Organ Cult. 32, 131-135.

Sujatha M. 1997. In vitro adventitious shoot regeneration for effective maintenance of male sterile niger (Guizotia abyssinica (L.F.) Cass.). Euphytica 93, 89-95.

Taji AM, Williams RR. 1996. Over view of tissue culture. In: Taji A, Williams R, ed. Tissue culture of Australian plants. Armidale, UK: University of  New England press, 174-183.

Tange GX, Zhou WJ, Li HZ, Mao BZ, He ZH, Yoneyama K. 2003. Medium, explant and genotype factors  influencing  shoot  regeneration  in  oil  seed Brassica spp. J. Agronomy and Crop Sci. 189, 351-358.

Zhang GQ, ZhouWJ, Gu HH, Song WJ, Momoh EJ. 2003. Plant regeneration from the hybridization of  Brassica  juncea  and  B.  napus  through  embryo culture.  J.  Agronomy  and  Crop  Sci.  189,  347-350.