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In vitro release behaviour of spray dried flaxseed oil Microcapsules for application in food system

Research Paper | May 1, 2019

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Muhammad Zia Shahid, Muhammad Imran, Muhammad Kamran Khan, Abdullah Ijaz Hussain

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Int. J. Biosci.14( 5), 205-216, May 2019

DOI: http://dx.doi.org/10.12692/ijb/14.5.205-216


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In recent era, demand for plant-based polyunsaturated fatty acids (PUFAs) has increased rapidly due to changing lifestyle and dietary requirements. Flaxseed oil with the highest level of PUFAs, is more likely to be oxidized if handled or stored inappropriately. Presently, encapsulation of oils by various encapsulation techniques has gaining popularity as a promising way of preservation. With the intent current research was planned to encapsulate FSO using spray drying method. Flaxseed meal was subjected to gum extraction using ultrasound technique. Two different sets of emulsion samples were prepared i.e.; flaxseed oil (FSO)/regular dried polysaccharide gum (RDPSG) (for model 1 capsule) and flaxseed oil (FSO)/freeze dried polysaccharide gum (FDPSG) (for model 2 capsule). The resultant spray dried flaxseed oil (SDFSO) samples obtained from both models were subjected to physical characterization including moisture content, water activity and encapsulation efficiency. Both model typesof microcapsules exhibited good encapsulation efficiency as 90.45±2.67 and 92.62±4.28% for FSO/RDPSG and FSO/FDPSG, accordingly. The effect of the wall material on digestibility of SDFSO microcapsules was also investigated using simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) models. The values observed for oil release in SGF were significantly higher in both models of SDFSO preparation when incubation was accompanied by heating. Similarly, A higher oil release percentage was found for FSO/FDPSG preparation as 65±3.95 with heating for 360 min followed by 45±3.42% without heating accordingly when observed under sequential (SGF+SIF) conditions. Flaxseed gums-based wall material was effective in carrying and delivering the flaxseed oil through gastrointestinal tract (GIT).


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In vitro release behaviour of spray dried flaxseed oil Microcapsules for application in food system

Ahn JH, Kim YP, Lee YM, Seo EM, Lee KW, Kim HS. 2008. Optimization of microencapsulation of seed oil by response surface methodology. Food Chemistry 107(1), 98-105.

AOAC. 2006. Official Methods of Analysis, 18th edition. Association of Official Analytical Chemists, Gaithersburg, Maryland.

AOCS. 1998. Official Methods and Recommended Practices of AOCS, 5th edition. American Oil Chemists Society, Champaign, IL, USA.

Augustin MA, Sanguansri L. 2012. Challenges in developing delivery systems for food additives, nutraceuticals and dietary supplements. Encapsulation technologies and delivery systems for food ingredients and nutraceuticals. Woodhead Publishing., 19-48.

Baker EJ, Miles EA, Burdge GC, Yaqoob P, Calder PC. 2016. Metabolism and functional effects of plant-derived omega-3 fatty acids in humans. Progress in Lipid Research 64, 30-56.

Bakry AM, Abbas S, Ali B, Majeed H, Abouelwafa MY, Mousa A, Liang L. 2016. Microencapsulation of oils: a comprehensive review of benefits, techniques, and applications. Comprehensive Reviews in Food Science and Food Safety 15(1), 143-82.

Binsi PK, Nayak N, Sarkar PC, Jeyakumari A, Ashraf PM, Ninan G, Ravishankar CN. 2017. Structural and oxidative stabilization of spray dried fish oil microencapsulates with gum arabic and sage polyphenols: Characterization and release kinetics.  Food Chemistry 219, 158-68.

Burgar MI, Hoobin P, Weerakkody R, Sanguansri L, Augustin MA. 2009. NMR of microencapsulated fish oil samples during in vitro digestion. Food biophysics 4(1), 32-41.

Calvo P, Castaño ÁL, Lozano M, González-Gómez D. 2012. Influence of the microencapsulation on the quality parameters and shelf-life of extra-virgin olive oil encapsulated in the presence of BHT and different capsule wall components. Food Research International 45(1), 256-61.

Carneiro HC, Tonon RV, Grosso CR, Hubinger MD. 2013. Encapsulation efficiency and oxidative stability of flaxseed oil microencapsulated by spray drying using different combinations of wall materials. Journal of Food Engineering 115(4), 443-51.

Chang JP, Su KP, Mondelli V, Pariante CM. 2018. Omega-3 polyunsaturated fatty acids in youths with attention deficit hyperactivity disorder: a systematic review and meta-analysis of clinical trials and biological studies. Neuropsychopharmacology 43(3), 534.

Comunian TA, Favaro-Trindade CS. 2016. Microencapsulation using biopolymers as an alternative to produce food enhanced with phytosterols and omega-3 fatty acids: A review. Food Hydrocolloids 61, 442-57.

Deglaire A, Bos C, Tomé D, Moughan PJ. 2009. Ileal digestibility of dietary protein in the growing pig and adult human. British Journal of Nutrition 102(12), 1752-9.

Dinu M, Abbate R, Gensini GF, Casini A, Sofi F. 2017. Vegetarian, vegan diets and multiple health outcomes: a systematic review with meta-analysis of observational studies. Critical reviews in food science and nutrition 57(17), 3640-9.

Domian E, Brynda-Kopytowska A, Marzec A.  2017. Functional Properties and Oxidative Stability of Flaxseed Oil Microencapsulated by Spray Drying Using Legume Proteins in Combination with Soluble Fiber or Trehalose. Food and Bioprocess Technology 10(7), 1374-86.

Gallardo G, Guida L, Martinez V, López MC, Bernhardt D, Blasco R, Pedroza-Islas R, Hermida LG. 2013. Microencapsulation of linseed oil by spray drying for functional food application. Food Research International 52(2), 473-82.

Gallier S, Singh H. 2012. The physical and chemical structure of lipids in relation to digestion and absorption. Lipid Technology 24(12), 271-3.

Gifford KD. 2002. Dietary fats, eating guides, and public policy: history, critique, and recommendations. The American Journal of Medicine 113(9), 89-106.

Gouin S. 2004.Microencapsulation: industrial appraisal of existing technologies and trends. Trends in food science & technology 15(7-8), 330-47.

Goyal A, Sharma V, Sihag MK, Arora S, Singh AK, Sabikhi L. 2016. Effect of microencapsulation and spray drying on oxidative stability of flaxseed oil and its release behavior under simulated gastrointestinal conditions. Drying Technology 34(7), 810-21.

Hu L, Zhang J, Hu Q, Gao N, Wang S, Sun Y, Yang X. 2016. Microencapsulation of bruceajavanica oil: Characterization, stability and optimization of spray drying conditions. Journal of Drug Delivery Science and Technology 36, 46-54.

Hur SJ, Decker EA, McClements DJ. 2009. Influence of initial emulsifier type on microstructural changes occurring in emulsified lipids during in vitro digestion. Food Chemistry 114(1), 253-62.

Jafari SM, Assadpoor E, Bhandari B, He Y.  Nano-particle encapsulation of fish oil by spray drying. Food Research International 41(2), 172-83.

Keogh MK, O’kennedy BT, Kelly J, Auty MA, Kelly PM, Fureby A, Haahr AM. 2001. Stability to oxidation of spray dried fish oil powder microencapsulated using milk ingredients. Journal of Food Science 66(2), 217-24.

Kosaraju SL, Weerakkody R, Augustin MA. 2009. In-vitro evaluation of hydrocolloid–based encapsulated fish oil. Food Hydrocolloids 23(5), 1413-9.

Let MB, Jacobsen C, Meyer AS. 2005. Sensory stability and oxidation of fish oil enriched milk is affected by milk storage temperature and oil quality. International Dairy Journal 15(2), 173-82.

Lipilina E, Ganji V. 2009. Incorporation of ground flaxseed into bakery products and its effect on sensory and nutritional characteristics–a pilot study. Journal of Foodservice 20(1), 52-9.

Lukaszewicz  M, Matysiak-Kata I, Skala J, Fecka I, Cisowski W, Szopa J. 2004. Antioxidant capacity manipulation in transgenic potato tuber by changes in phenolic compounds content. Journal of Agricultural and Food Chemistry 52(6), 1526-33.

Masurkar AA, Datar AG, Vakil BV. 2014. Screening and identification of PUFA producers from various fish of indian coastal and fresh water habitats. International Journal of Life Sciences Biotechnology and Pharma Research 3(3), 181.

Masurkar AA, Datar AG, Vakil BV. 2015. New Microbial Sources of Pufa From the Arabian Sea and Fresh Water Habitats of Indian Territory. International Journal of Pharmaceutical Sciences and Research 6(1), 349-60.

Mun S, Decker EA, McClements DJ. 2007.  Influence of emulsifier type on in vitro digestibility of lipid droplets by pancreatic lipase. Food Research International 40(6), 770-81.

Murakami K, Mizoue T, Sasaki S, Ohta M, Sato M, Matsushita Y, Mishima N. 2008. Dietary intake of folate, other B vitamins, and ω-3 polyunsaturated fatty acids in relation to depressive symptoms in Japanese adults. Nutrition 24(2), 140-7.

Omar KA, Shan L, Zou X, Song Z, Wang X. 2009. Effects of two emulsifiers on yield and storage of flaxseed oil powder by response surface methodology. Pakistan Journal of Nutrition 8(9), 1316-24.

Özbek ZA, Ergönül PG. 2017. A review on encapsulation of oils. Celal Bayar Üniversitesi Fen Bilimleri Dergisi 13(2), 293-309.

Polavarapu S, Oliver CM, Ajlouni S, Augustin MA. 2011. Physicochemical characterisation and oxidative stability of fish oil and fish oil–extra virgin olive oil microencapsulated by sugar beet pectin. Food Chemistry 127(4), 1694-705.

Popa VM, Gruia A, Raba DN, Dumbrava D, Moldovan C, Bordean D, Mateescu C. 2012. Fatty acids composition and oil characteristics of linseed (LinumUsitatissimum L.) from Romania. Journal of Agroalimentary Processes and Technologies 18(2), 136-40.

Quispe-Condori S, Saldaña MD, Temelli F. 2011. Microencapsulation of flax oil with zein using spray and freeze drying. LWT-Food Science and Technology 44(9), 1880-7.

Rajaram S, Valls-Pedret C, Cofán M, Sabaté J, Serra-Mir M, Pérez-Heras AM, Arechiga A, Casaroli-Marano RP, Alforja S, Sala-Vila A, Doménech M. 2017. The Walnuts and Healthy Aging Study (WAHA): protocol for a nutritional intervention trial with walnuts on brain aging. Frontiers in aging neuroscience 8, 333.

Siscovick DS, Barringer TA, Fretts AM, Wu JH, Lichtenstein AH, Costello RB, Kris-Etherton PM, Jacobson TA, Engler MB, Alger HM, Appel LJ. 2017. Omega-3 polyunsaturated fatty acid (fish oil) supplementation and the prevention of clinical cardiovascular disease: a science advisory from the American Heart Association. Circulation 135(15), 867-84.

Timilsena YP, Adhikari R, Barrow CJ, Adhikari B. 2017. Digestion behaviour of chia seed oil encapsulated in chia seed protein-gum complex coacervates. Food hydrocolloids 66, 71-81.

US Pharmacopeial Convention. US Pharmacopeia and National Formulatory. 2000. USP 24 NF 19. Rockville, MD.

Wang XW, Hai H, Jin WY, Wang K, Yang D. 2010. Optimization of microwave-ultrasound assisted extraction of flavonids from ginkgo biloba leaves by response surface methodology. Food Science and Technology 3, 058.

Wickham M, Wilde P, Fillery-Travis A. 2002. A physicochemical investigation of two phosphatidylcholine/bile salt interfaces: implications for lipase activation. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids 1580(2-3), 110-22.