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In vitro study of some characteristics of red blood cells with hemoglobin S

Research Paper | January 1, 2017

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Sènou M, Lokonon JE, Agbogba F, Agbangnan DCP, Odoulami S, Tchogou AP, Lalèyè A, Sèzan A, Loko F

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Int. J. Biosci.10( 1), 311-317, January 2017

DOI: http://dx.doi.org/10.12692/ijb/10.1.311-317


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Sickle cell anemia is hemoglobin sis due to mutated S hemoglobin widespread in the black race. The aim of this work was to determine some biological properties of erythrocytes with hemoglobin S in order to monitor the efficacy of anti-sickle cell traditional treatments. 393 patients were taken from the Zou regional hospital in Benin for hemoglobin electrophoresis, the Emmel test and the osmotic resistance of red blood cells. The hemoglobin electrophoresis phenotyped 63.87% AA, 17.81% AS, 10.94% AC, 2.80% SS, 4.07% SC and 0.51% CC. The Emmel test was positive in 100% of SS cases, 90% of AS cases and 93.75% of SC cases. The rate of sickle cell formation was less than one hour in 100% SS and 72.73% SC. In the AS phenotype, the appearance of sickle-cell was rather progressive (25% of cases in one hour, 35% in two hours, 20% in three hours and 20% in four hours) suggesting competition between both types of hemoglobin. Compared to the AA phenotype, the osmotic resistance of the red cells increased significantly in the AS phenotype and very significantly in the SS and SC phenotypes, indicating an increased erythropoiesis compensating the sickle cell hemolysis. Hemoglobin S was associated with an excellent osmotic resistance of red blood cells and the rate of sickle cell formation depended on the hemoglobin phenotype. These two parameters can be used to monitor the efficacy of traditional remedies proposed to treat sickle cell anemia.


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In vitro study of some characteristics of red blood cells with hemoglobin S

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