In vivo assessment of toxicological potential of graphene oxide nanosheets in Sprague Dawley rats

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In vivo assessment of toxicological potential of graphene oxide nanosheets in Sprague Dawley rats

Zahid Iqbal, Farhat Jabeen, Tanveer A. Tabish, Tayyaba Sultana, Salma Sultana
Int. J. Biosci.15( 2), 456-474, August 2019.
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Graphene oxide nanosheets (GON) have encouraging applications in the field of biology, particularly in drugs delivery and therapeutics due to its distinctive properties. Despite of its greater applications in vivo studies but data of toxicity in vivo is scares. Therefore, a study was designed to assess the in vivo toxic potential of GON in Sprague Dawley rats by involving 25 rats distributed into 5 groups having 5 replicates. The groups were named as control (without any treatment), placebo (receiving deionized water intraperitoneally) and three treated groups (G1-G3) exposed with GON intraperitoneally @ 1.5 or 2.5 or 3.5mg/kg of bodyweight on alternate day for 30 days. After 30 days of exposure toxicity induced by GON was assessed. No death or change in body morphology and behavior were observed during the whole experiment. In present study, exposure of GON @ 2.5 and 3.5mg/kg BW induced toxicity, which was evident by the alteration in somatic index of liver, liver function enzymes concentrations (ALT, AST and ALP), markers of oxidative stress (MDA and LPO), enzymes of antioxidative system (CAT and GSH) and histopathology of liver of treated groups compared with control. Normal histology was observed in control and deionized treated rats while treated rats with GON showed dilation in central vein, pyknotic nuclei and degeneration of hepatocytes in a dose dependent manner. Therefore, much attention is required for the investigation of dose dependent toxicity of GON so that strictly monitored dose could be used in vivo applications.


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