Welcome to International Network for Natural Sciences | INNSpub

In vivo, in vitro antioxidant and hypolipidemic activity of methanol extract of Pineapple peels in Wistar Rats

Research Paper | June 1, 2016

| Download 7

Ejiofor U. Emmanuel, Ebhohon S. Onagbonfeoana, Obike C. Adanma, Obinwanne C. Precious, Azubike-Izah Faith, Omeh Y. Ndukaku

Key Words:

Int. J. Biosci.8( 6), 64-72, June 2016

DOI: http://dx.doi.org/10.12692/ijb/8.6.64-72


IJB 2016 [Generate Certificate]


The medicinal values of pineapple has been well documented in literature. However, pineapple peels constitute the major waste of the pineapple fruit. Recent interest has been on harnessing plants waste materials for economic and industrial values. The study investigated the in vivo, in vitro and hypolipidaemic activity of pineapple peel extract on wistar albino rats. Twenty wistar albino rats grouped into four groups were used for the study. Group A served as control group, while group C-D received varying doses of the crude extract (200, 300 and 500mg/kg body weight). Experimental period lasted for twenty eight days, after which animals were fasted overnight and blood collected via ocular puncture for lipid profile analysis and liver obtained for antioxidant analysis. The in vitro antioxidant activity was determined using 2, 2- diphenyl-1-picrylhydrazine photometric assay and ferric reducing ability assay. Result indicated a concentration dependent (25- 400 µg/mL) for the extract in 2, 2- diphenyl-1-picrylhydrazine photometric assay and ferric reducing ability assay. In vivo antioxidant assay showed no significant (P<0.05) difference in the extract administered group when compared to the control group. Serum HDL estimation showed a significant (P<0.05) increase in the extract administered group (200 and 500mg/kg) when compared to the control group. Result for in vivo showed that the extract did not induce oxidative stress and can be a positive agent in preventing lipid peroxidation while increase in functional HDL indicated that pineapple peels may be useful in the management of hyperlipidaemia and other associated diseases.


Copyright © 2016
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

In vivo, in vitro antioxidant and hypolipidemic activity of methanol extract of Pineapple peels in Wistar Rats

Allian C, Lucy S, Cicely S, Chan W, Paul C. 1974. Enzymatic determination of total serum cholesterol. Clinical Chemistry, 470- 475.

Arulselvan P, Subramanian S. 2007. Beneficial effects of Murraya koenigii leaves on antioxidant defense system and ultra-structural changes of pancreatic beta cells in experimental diabetes in rats. Chemico- Biological Interactions 165(2), 155- 164.

Assmann G, Schriewer H, Schmitz G, Hadge E. 1983. Quantification of HDL-C by precipitation with phosphotungstic acid and MgCl2. Clinical Chemistry. 29, 2026- 2030.

Atawodi S. 2011. Evaluation of the hypoglycaemic, hypolipidemic and antioxidant effects of methanolic extract of “Ata-Ofa” polyherbal tea (A- polyherbal) in alloxan- induced diabetic rats. Drug Invention Today 3, 270- 276.

Atul U, Jeewan P, Shinkichi T. 2010. Utilization of pineapple waste: a review. Journal of Food Science and Technology 6, 10- 18.

Bakirel T, Bakirel U, Keles O, Ulgen S, Yardibi H. 2008. In- vivo assessment of antidiabetic and antioxidant activities of Rosemary (Rosmarinus officinalis) in alloxan- diabetic rabbits. Journal of Ethnopharmacology 116(1), 39- 42.

Bardiya N, Somayaji D, Khanna S. 1996. Biomethanation of banana peel and pineapple waste. Bioresource Technology 58, 73-76.

Bartholomew D, Paull R, Rohrbach K. 2003. The Pineapple: Botany, Production and Uses. CABI Publishing, Wallingford, UK. 21.

Benzie F, Strain J. 1999. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology 299, 15-23.

Chelikani P, Fita I, Loewen P. 2004. Diversity of structures and properties among catalase. Cellular and Molecular Life Sciences 61(2), 192- 208.

Chisolm G. 2001. The oxidative modification hypothesis of atherogenesis: an overview. Free Radical Biol. Med. 28, 1815–1826.

Dacera D, Babel S, Parkpian P. 2009. Potential for land application of contaminated sewage sludge treated with fermentaed liquid from pineapple wastes. Journal of Hazardous Materials. 167, 866-872.

DHHS. 1985. Guide for the care and use of laboratory animals, institute of laboratory animal resources commission on life sciences, National Research Council. Washington DC, National academy press.

Diplock A, Charleux J, Crozier-Willi G. 1998. Functional food science and defense against reactive oxidative. British Journal of Nutrition. 80(1), 77-112.

Draper H, Hadley M. 1990. Malondialdehyde determination as index of lipid peroxidation. Methods in Enzymology 186, 421- 431.

Eklund P, Langvik O, Warna J, Salmi T, Willfor S. 2005. Chemical studies on antioxidant mechanisms and free radical scavenging properties of lignans. Organic and Bimolecular Chemistry 21, 3336–3347.

Emmanuel UE, Ebhohon OS, Omeh YN. 2015. Effect of fermented and unfermented cocoa bean on some liver enzymes, creatinine and antioxidant in wistar albino rats. Carpathian Journal of Food Science and Technology 7(4), 132-138.

Gerd A, Antonio M, Gotto J. 2004. HDL cholesterol and protective factors in atherosclerosis. Circulation. 109, 8- 14.

Guo C, Yang J, Wei J, Li Y, Xu J. 2003. Antioxidant activities of peel, pulp, and seed fractions of common fruits as determined by FRAP assay. Nutrition Research 23, 1719-1726.

Halliwell B, Gutteridge J. 1999. Free radicals in biology and medicine, Oxford University press UK.

Hanukoglu I. 1992. Steroidogenic enzymes: structure, function and role in regulation of steroid hormone biosynthesis. Journal of Steroid Biochem. Mol. Biol. 102(2), 306- 310.

Hemalatha R, Anbuselvi S. 2013. Physicochemical constituent of pineapple pulp and waste. Journal of Chemical and Pharmaceutical Research 5(2), 240- 242.

Hermansen K, Dinesen B, Hoie L, Morgenstern E, Gruenwald J. 2003. Effects of soy and other natural products on LDL: HDL ratio and other lipid parameters: A literature review. Advances in Therapy 20(1), 50- 78.

Hossain A, Rahman S. 2010. Total phenolics, flavonoids and antioxidant activity of tropical fruit pineapple. Food Research International, 1–7.

Ijeh I, Ejike C. 2011. Current perspectives on the medicinal potentials of Vernonia amygdalina Del. Journal of Plant Medicinal Research 5(7), 1051- 1061.

Iris F, Strain J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of ‘‘Antioxidant Power’’: The FRAP assay. Analytical Biochemistry 239, 70–76.

Jasmine P, Estherlydia D. 2014. Comparative study of phytochemical screening and antioxidant capacities of vinegar made from peel and fruit of pineapple (Ananas comosus L.). International Journal of Pharma and Bio Sciences 5(4), 394- 403.

Kaushik D, Aryadeep R. 2014. Reactive oxygen species (ROS) and response of antioxidnats as ROS-scavengers during environmental stress in plants. Frontiers in Environmental Science 2, 53.

Maritim A, Sanders R, Watkins J. 2003. Diabetes, oxidative stress and antioxidants: A review. Journal of Biochem. and Mol. Toxicology 17(1), 24-38.

Mensor L, Fabio S, Gilda G, Alexandre S, Tereza C. 2001. Screening of Brazilian plants extracts for antioxidants activity by the use of DPPH free radical method. Phytother. Res. 15, 127-130.

Milton N. 2004. Role of hydrogen peroxide in the aetiology of Alzheimer’s disease: implications for treatment. Drugs Aging 21(2), 81- 100.

Ochuko E, John A, Rachael A, Oluwatoyin O, Sunday A. 2011. Protective effect of pineapple (Ananas cosmosus) peel extract on alcohol-induced oxidative stress in brain tissues of male albino rats. Asian Pacific Journal of Tropical Disease 11, 2-9.

Prasenjit D, Prasanta D, Abhijit C, Tejendra B. 2012. A survey on pineapple and its medicinal value. Scholars Academic Journal of Pharmacy 1(1), 24-29.

Rui-Li Y, Yong-Hui S, Gang H, Wu L, Guo-Wei L. 2008. Increasing oxidative stress with progressive hyperlipidemia in human: relation between malondialdehyde and atherogenic index. Journal of Clinical Biochemistry and Nutrition 48(3), 154-158.

Sukhdev S, Suman P, Gennaro L, Dev R. 2008. Extraction techniques for medicinal and aromatic plants. International center for science and high technology.

Sun Y, Oberley W, Li Y. 1988. A simple method for clinical assay of superoxide dismutase. Clinical Chemistry 34(3), 497- 500.

Ti L, Peiyi S, Wei L, Chengmei L, Ruihong L, Na Y, Jun C. 2014. Major polyphenolics in pineapple peels and their antioxidant interactions. International Journal of Food Properties 17(8), 1805-1817. http://dx.doi.org/10.1080/10942912.2012.732168

Tietz N. 1995. Clinical guide to laboratory tests, 3rd ed, W B Sanders Company Philadelphia U.S.A, 578-580.

Wijeratne S. 2005. Hydrogen peroxide induced oxidative stress damage and antioxidant enzyme response in Caco- 2 human colon cells. Journal of Agricultural and Food Chemistry 53(22), 8768-8774.