Incidence and distribution of begomoviruses from pothwar region of Pakistan

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Research Paper 01/11/2016
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Incidence and distribution of begomoviruses from pothwar region of Pakistan

Hasan Riaz, Muhammad Ashfaq, Tariq Mukhtar, Muhammad Kausar Nawaz Shah, Saad Imran Malik
J. Biodiv. & Environ. Sci. 9(5), 11-16, November 2016.
Copyright Statement: Copyright 2016; The Author(s).
License: CC BY-NC 4.0

Abstract

Begomoviruses are single stranded whitefly transmitted plant viruses which are responsible for huge economic losses across the world. Several begomovirus infections have been reported from Pakistan in last two decades but there are very less reports of begomoviruses from Pothwar region which lies on the north-eastern part of Pakistan with very diverse vegetation. The study was aimed to estimate the incidence and distribution of begomoviruses in Pothwar region. Plant samples from 17 different plant families were collected and followed by PCR analysis by different primer sets in order to identify the presence of begomoviruses in tested plant samples. The presence of begomoviruses was confirmed in 15 plant families with 77.5% of an overall incidence of begomoviruses and uneven distribution was observed with maximum 32 % in Rawalpindi district of Pothwar region. The incidence was also calculated across plant families where family Solanaceae topped the list with 92 % incidence. The overall picture in Pothwar region was much favourable for begomoviruses spread and their vector whitefly. In present study the results is a glimpse of the huge damage to plants caused by begomoviruses. The region must be thoroughly combined for begomoviruses with large amount of plant samples covering more plant families.

Akhter A, Qazi J, Saeed M, Mansoor S. 2009. A  Severe Leaf Curl Disease on Chilies in Pakistan is Associated with Multiple Begomovirus Components. Plant Disease 93, 962.

Brown JK, Zerbini MF, Navas-Castillo J, Moriones E, Ramos-Sobrinho R, Silva JCF, Fiallo-Olive E, Briddon RW, Herna´ndez-epeda C, Idris A, Malathi VG, Martin DP, Rivera-Bustamante R, Ueda S, Varsani A. 2015. Revision of Begomovirus taxonomy based on pairwise sequence comparisons. Archives of Virology 160, 1593-1619.

Brown JK, Fauquet CM, Briddon RW, Zerbini FM, Moriones E, Navas-Castillo J. 2012. Family Geminiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ. Virus taxonomy: Ninth report of the international committee on taxonomy of viruses. London, UK: Elsevier Academic Press. 351-373 p.

Briddon RW, Mansoor S, Bedford ID, Pinner MS, Markham PG. 2000. Clones of Cotton leaf curl geminivirus induce symptoms atypical of cotton leaf curl disease. Virus Genes 20, 17– 24.

Doyle JJ, Doyle JL. 1987. A rapid DNA isolation from small amount of fresh leaf tissue. Phytochemical Bulletin 19, 11– 15.

Edwards LE. 2007. Paleocene and Eocene dinocysts from the Salt Range, Punjab, northern Pakistan. In: War­wick PD, Wardlaw BR. Regional studies of the Potwar Plateau area, northern Pakistan: U.S. Geological Survey Bulletin., p. 2078– 2079.

Haider MS. 1996. Characterization of Whitefly transmitted geminiviruses from Pakistan. PhD Thesis, University of London, United Kingdom, 65– 66.

Hameed S, Robinson DJ. 2004. Begomoviruses from mungbeans in Pakistan: epitope profiles, DNA A sequences and phylogenetic relationships. Archives of Virology 149, 809– 819.

Ilyas M, Qazi J, Mansoor S, Briddon RW. 2010. Genetic diversity and phylogeography of begomoviruses infecting legumes in Pakistan. Journal of General Virology 91, 2091– 2101.

Ilyas M, Nawaz K, Shafiq M, Haider MS, Shahid AA. 2013. Complete nucleotide sequences of two begomoviruses infecting Madagascar periwinkle (Catharanthus roseus) from Pakistan. Archives of Virology 158, 505– 510.

Kil E, Kim S, Lee Y, Byun H, Park J, Seo H, Kim C, Shim J, Lee J, Kim J, Lee K, Choi H, Lee S. Tomato yellow leaf curl virus (TYLCV-IL): a seed-transmissible geminivirus in tomatoes. Scientific Reports 6, 19013. http://dx.doi.org/10.1038/srep19013.

Mansoor S, Khan SH, Bashir A, Saeed M, Zafar Y, Malik KA, Briddon RW, Stanley J, Markham PG. 1999. Identification of a novel circular single-stranded DNA associated with cotton leaf curl disease in Pakistan. Virology 259, 190– 199.

Mustujab A, Briddon RW, Tahir M. 2015. First report of Cathranthus yellow mosaic virus infecting Duranta repens in Pakistan. Journal of Plant Pathology 97, 209– 220.

Nawaz-ul-Rehman MS, Briddon RW, Fauquet CM. 2012. A Melting Pot of Old World Begomoviruses and Their Satellites Infecting a Collection of Gossypium Species in Pakistan. PLoS ONE 7, e40050. http://dx.doi.org/10.1371/journal. pone.0040050.

Shih SL, Tsai WS, Green SK, Khalid S, Ahmad I, Rezaian MA, Smith J. 2003. Plant Disease 87, 200.

Stanley J, Bisaro DM, Briddon RW, Brown JK, Fauquet CM, Harrison BD, Rybicki EP, Stenger DC. 2005. Geminiviridea In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA. Virus Taxonomy: VIIIth Report of the International Committee on Taxonomy of Viruses. London, UK, Elsevier Academic Press, 1163– 1169 p.

Tahir MN, Amin I, Briddon RW, Mansoor S. 2011. The merging of two dynasties-identification of an African cotton leaf curl disease-associated begomovirus with cotton in pakistan. PLoS ONE 6, 20366.

Wyatt SD, Brown JK. 1996. Detection of Subgroup III Geminivirus isolates in Leaf Extracts by Degenerate Primers and Polymerase Chain Reaction. Phytopathology 86, 1288– 1293.

Zhou XP, Liu Y, Robinson DJ, Harrison BD.  1998. Four DNA-A variants among Pakistani isolates of cotton leaf curl virus and their affinities to DNA-A of geminivirus isolates from Okra. Journal of General Virology 79, 915– 923.

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