Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | March 1, 2015

| Download 2

Increasing of Chavicol o-methyl transferase gene expression (CVOMT) and methyl Chavicol value of basil (Ocimum basilicum) by salicylic acid

Hemadollah Zarei, Barat Ali Fakheri, Sedigheh Esmaeilzadeh Bahabadi, Mahmood Solouki

Key Words:

J. Bio. Env. Sci.6(3), 46-53, March 2015


JBES 2015 [Generate Certificate]


Basil (Ocimum basilicum), is a medicinal plant mint family (Lamiaceae) which contains cyclic compounds and essential oil, and it has antibacterial and antioxidant properties. From Long ago, Basil has been used for treatment of headache, cough, diarrhea and fever, inflammation of the throat and stomach pain in Iran. Basil essential oil is mainly containing phenylpropanoids compounds, the biosynthesis path of these compounds are of course passed from the pathway of shikimate and the most important compounds of them, that can be cited, are chavicol and methyl chavicol. The role of salicylic acid is known as a bio- stimulant to improve the biosynthesis of secondary metabolites in many plants. In this study, the effect of salicylic acid on gene expression CVOMT and the amount of methyl chavicol was studied. Therefore, basil was treated with 2 mM solution of salicylic acid before flowering stage, and respectively the samples were harvested 1, 2, 3 and 5 days after treatment. Gene expression CVOMT which was under influence of salicylic acid, compared with controls, showed the highest activity of gene expression CVOMT. So, on the third day after treatment was reached to highest level and on the fifth day after treatment was reduced. Essential oil analysis also showed that the methyl chavicol influenced by salicylic acid, in comparing with controls, like gene expression (CVOMT) was increased. In General, the changes in gene expression CVOMT and the amount of methyl chavicol that harvested at different stages were Conformity.


Copyright © 2015
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Increasing of Chavicol o-methyl transferase gene expression (CVOMT) and methyl Chavicol value of basil (Ocimum basilicum) by salicylic acid

Adams RP. 2001. Identification of Essential Oils Components by Gas Chromatography/ Quadrupole Mass Spectroscopy, Allured Publishing Co., Illinois, USA

Biavati B, Piccaglia R, Marotti M. 2005 antimicrobial activity of plant essential oils, Food Chemistry 92, 128-137.

Chalchat JC, Ozen M. 2008. Comparative essential oil composition of flower, leaves and stems of basil (Ocimum basilicum L.) used as herb. Journal of Food Chemistry 110, 501-503.

Coquoz JL, Buchala A, Metraux JP. 1998. The biosynthesis of salicylic acid in potato plants. Journal of Plant Physiology 117, 1095–11010.

El-Tayeb MA. 2005. Response of barley gains to the interactive effect of salinity and salicylic acid. Plant Growth Regulation 45, 215-225.

Gang DR, Wang J, Dudareva N, Nam KH, Simon JE, Lewinsohn E, Pichersky E. 2001. An Investigation of the Storage and Biosynthesis of Phenylpropenes in Sweet Basil, Plant Physiology 125, 539-555.

Ghahraman A. 1994. Iran Cormophytes (systematic plant), Iran University Press 3, pp89, (in Persian).

Hayat Q, Hayat S, Irfan M, Ahmad Q. 2009. Effect of exogenous salicylic acid under changing environment. Environmental and Experimental Botany 68, 14-25.

Iijima Y, Davidovich-Rikanati R, Fridman E, Gang DR, Bar E, Lewinsohn E, Pichersky E. 2004. The Biochemical and Molecular Basis for the Divergent Patterns in the Biosynthesis of Terpenes and Phenylpropenes in the Peltate Glands of Three Cultivars of Basil. Journal of Plant Physiology 136, 3724-3736.

Juliani HR, Simon JE. 2002. Antioxidant Activity of Basil, Trends in new crops and new uses, 575- 579.

Kang C, Wang Ch. 2003. Salicylic acid changes activities of H2O2 metabolizing enzymes and increases the chilling tolerance of banana seedling. Environment and Experimental Botany 50, 9-15.

Kang S, Jung H, Bahk J, Yang J. 2004. Effects of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopolia parviflora. Journal of Plant Science 166, 745-751.

Labra M, Miele M, Ledda B, Grassi F, Mazzei M, Sala F. 2004. Morphological characterization, essential oil composition and DNA genotyping of Ocimum basilicum L. cultivars, Plant Science 167, 725-731.

Lewinsohn E, Ziv-Raz I, Dudai N, Tadmor Y, Lastochkin E, Larkov O, Chaimovitsh D, Ravid U, Putievsky E, Pichersky E, Shoham Y. 2000. Biosynthesis of estragole and methyl- eugenol in sweet Basil (Ocimum basilicum L.) Developmental and chemotypic association of allylphenol O-methyltransferase activities, Journal of Plant Science 160, 27-35.

Marotti M, Piccaglia R, Giovanelli E. 1996. Differences in essential oil composition of basil (Ocimum basilicum L.) Italian cultivars related to morphological characteristics. Journal of Agricultural and Food Chemistry 44, 3926-3929.

Mc Donland T, Alexeef GV, Zeise L, Sandy SM, Wang Y. 1999. Evidence on the carcinogenicity of estragole, reproductive and cancer hazard assessment and California environmental protection agency. Journal of Crop Improvement 46, 748-752.

Omid baigi R. 2000. Production and Processing of Medicinal Plants, Astan Quds Razavi Press 3, pp73 (in Persian).

Poor bozorgi Roudsari N. 2007. Survey on Quantitative and Qualitative of Essential oil and Comparison Gene Expression of Chavicol o- methyl transferase, in two Varieties of Iranian Basil, Master Thesis, Plant Biology, Faculty of Science, Tarbiat Modarres University, (in Persian).

Popova L, Ananieva V, Hristova V, Christov K, Georgieva K, Alexieva V, stoinova Zh. 2003. Salicylic acid and Methyl jasmonate induced protection on photosynthesis to paraquat oxidative stress. Bulgarian Journal of plant physiology, special issue, 133-152.

Popova L, Pancheva T, Uzunova A. 1997. Salicylic acid: Properties, Biosynthesis and Physiological Role. Journal of Plant Physiology 23, 85-93.

Pu GB, Dong-Ming M, Chen JL, Ma LQ. 2009. Salicylic acid activates artemisinin biosynthesis in Artemisia annua L. Plant Cell Reports 28, 1127–1135.

Raskin I. 1992.  Role  of  salicylic  acid  in  plants. Journal of Plant Physiology 99, 799-803.

Sajjadi SE. 2006. Analysis of essential oils of two cultivated basil (Ocimum basilicum L.) from iran. Daru, 14, 1-3.

Sangwan NS, Farooqi AHA, Shabih F, Sangwan RS. 2001. Regulation of essential oil production in plants. Plant Growth Regulation 34, 3-21.

Shibamoto T, Sandra P, Bicchi C. 1987. Capillary gas chromatography in essential oil analysis, Huethig, New York.

Vina A, Murillo E. 2003. Essential oil composition from twelve varieties of basil (Ocimum spp.) grown in Colombia, Braz. Journal of Food Chemistry 5, 744-749.

Werker E, Putievsky E, Ravid U, Dudai N, Katzir I. 1993. Glandular h and essential oil in developing leaves of Ocimum basilicum L. (Lamiaceae). Annual Botany 71, 43-50.

Zargari, A. 1993. Medicinal Plants, University of Tehran Press 4, pp145, (in Persian).

Ziaei M, Sharifi M, Bahmanesh M, Razavi K. 2012. Gene expression and activity of Phenyl alanine ammonialyase and essential oil composition of Ocimum basilicum L. at different growth stage. Journal of Biotechnology 10, 32-39.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background