Indian ginseng (N.Hexane and Chloroform extracts) offers ameliorating effects on muscle functions restoration in a mouse model of peripheral nerve injury

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Research Paper 01/05/2021
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Indian ginseng (N.Hexane and Chloroform extracts) offers ameliorating effects on muscle functions restoration in a mouse model of peripheral nerve injury

Faiqa Sajid, Haseeb Anwar, Azhar Rasul, Ali Imran, Shoaib Ahmad Malik, Shamaila Zafar, Javeria Maqbool, Rabia Akram, Fazeela Ijaz, Amna Rashid Tariq, Ghulam Hussain
Int. J. Biosci.18( 5), 231-240, May 2021.
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Peripheral nerve injuries fall among most commonly happening medical situations nowadays. Such injuries occur in response to gunshots, road traffic accidents, mechanical crushes and traumas which eventually result into compromised sensory and motor activities. Despite having wonderful advancements in pharmacological therapies, such injuries are still waiting for their first line treatment. The present study is planned to assess the effect of n.Hexane and Chloroform extract of Indian ginseng root on sensorimotor function revival in mice induced with sciatic nerve crush lesion. Twelve male BALB/C mice were divided into control and 2 treatment groups having an equal number of mice (n=4) per group. Control was offered routine diet, while treatment groups (n.Hexane and Chloroform) were offered diet mixed with their respective treatments (100mg/kg daily dose of each extract of Indian ginseng root) from the day of the nerve crush and then until the end of the trial. Motor and sensory functions retrieval was assessed through sciatic functional index, grip strength, and hot plate tests which were found to be highly significant for Chloroform treated group. This group also showed prominent increase in skeletal muscle mass. The significant reduced (p=0.01) total oxidant status and increased total antioxidant capacity (p=0.01) in this group suggest that Indian ginseng root’s Chloroform extract has prominent potential for ameliorating the muscle function restoration as compared to control and n-Hexane group.


Alam N, Hossain M, Mottalib MA, Sulaiman SA, Gan SH, Khalil MI. 2012. Methanolic extracts of Withania somnifera leaves, fruits and roots possess antioxidant properties and antibacterial activities. BMC Complementary and Alternative Medicine 12, 1-8.

Dhuley JN. 1998. Effect of ashwagandha on lipid peroxidation in stress-induced animals. Journal of Ethnopharmacology 60(2), 173-178.

Durg S, Dhadde SB, Vandal R, Shivakumar BS, Charan CS. (2015, July). Withania somnifera (Ashwagandha) in neurobehavioural disorders induced by brain oxidative stress in rodents: A systematic review and meta-analysis. Journal of Pharmacy and Pharmacology, Vol 67, pp. 879-899.

Erel O. 2004. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry, 37(4), 277-285.

Erel O. 2005. A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38(12), 1103-11111.

Faroni A, Mobasseri SA, Kingham PJ, Reid AJ. 2015. Peripheral nerve regeneration: Experimental strategies and future perspectives. Advanced Drug Delivery Reviews, Vol. 82, pp. 160-167.

Gargiulo S, Gramanzini M, Megna R, Greco A, Albanese S, Manfredi C, Brunetti A. 2014. Evaluation of growth patterns and body composition in c57bl/6j mice using dual energy x-ray absorptiometry. BioMed Research International, 2014.

Gordon T, English AW. 2016. Strategies to promote peripheral nerve regeneration: Electrical stimulation and/or exercise. European Journal of Neuroscience 43(3), Khazdair, M. R., Anaeigoudari, A., Hashemzehi.

Grandhi A, Mujumdar AM, Patwardhan B. 1994. A comparative pharmacological investigation of Ashwagandha and Ginseng. Journal of Ethnopharmacology 44(3), 131-135.

Halter B, Gonzalez de Aguilar JL, Rene F, Petri S, Fricker B, Echaniz-Laguna A, Loeffler JP. 2010. Oxidative stress in skeletal muscle stimulates early expression of Rad in a mouse model of amyotrophic lateral sclerosis. Free Radical Biology and Medicine 48(7), 915-923.

Hussain G, Schmitt F, Henriques A, Lequeu T, Rene F, Bindler F, Loeffler JP. 2013. Systemic down-regulation of delta-9 desaturase promotes muscle oxidative metabolism and accelerates muscle function recovery following nerve injury. PloS One, 8(6), e64525.

Kalra R, Kaushik N. 2017. Withania somnifera (Linn.) Dunal: a review of chemical and pharmacological diversity. Phytochemistry Reviews 16(5), 953-987.

Kamble N, Shukla D, Bhat D. 2019. Peripheral Nerve Injuries: Electrophysiology for the Neurosurgeon. Neurology India Vol. 67, pp. 1419-1422.

Kamran SKS, Rasul A, Anwar H, Irfan S, Ullah KS, Malik SA, Hussain G. 2020. Ferula asafoetida Linn. is effective for early functional recovery following mechanically induced insult to the sciatic nerve of a mouse model. Tropical Journal of Pharmaceutical Research. 4314/tjpr.v19i9.15

Khuwaja G, Khanmm, Ishrat T, Ahmad A, Raza SS, Ashafaq M, Islam F. 2011. Neuroprotective effects of curcumin on 6-hydroxydopamine-induced Parkinsonism in rats: Behavioral, neurochemical and immunohistochemical studies. Brain Research, 1368, 254-263.

Kuboyama T, Tohda C, Komatsu K. 2005. Neuritic regeneration and synaptic reconstruction induced by withanolide A. British Journal of Pharmacology 144(7), 961-971. .1038/sj.bjp.0706122

Kuboyama T, Tohda C, Komatsu K. 2006. Withanoside IV and its active metabolite, sominone, attenuate Aβ(25-35)-induced neurodegeneration. European Journal of Neuroscience 23(6), 1417-1426.

Kulkarni SK, Dhir A. 2008. Withania somnifera: An Indian ginseng. Progress in Neuro-Psychopharmacology and Biological Psychiatry, Vol 32, pp. 1093-1105.

Kumar GP, Khanum F. 2012. Neuroprotective potential of phytochemicals. Pharmacognosy Reviews 6(12), 81-90.

Li R, Liu Z, Pan Y, Chen L, Zhang Z, Lu L. 2014. Peripheral nerve injuries treatment: a systematic review. Cell Biochemistry and Biophysics 68(3), 449-454.

Luca ADe. 2008. Use of grip strength meter to assess the limb strength of mdx mice. TREAT-NMD Neuromusclar Network, DMD_M.2.2.(2.0), 1-11.

Manjoosha S, Ashok K, Mahesh P, Division P, Marg RP. 2010. Phytochemical investigation on Jatropha curcas seed cake 1(91), 357-362.

Maqbool J, Anwar H, Iqbal J, Rasul A, Imran A, Ahmad Malik S, Islam S. 2020. Methanolic extract of Fennel (Foeniculum vulgare) escalates functional restoration following a compression injury to the sciatic nerve in a mouse model. Food Science and Nutrition, (October), 1-10. 1002/fsn3.2033

Osborne NR, Anastakis DJ, Davis KD. 2018. Peripheral nerve injuries, pain, and neuroplasticity. Journal of Hand Therapy 31(2), 184-194.

Pandeymm, Rastogi S, Rawat AKS. 2013. Indian traditional ayurvedic system of medicine and nutritional supplementation. Evidence-Based Complementary and Alternative Medicine, Vol. 2013.

Pingali U, Pilli R, Fatima N. 2014. Effect of standardized aqueous extract of Withania somniferaon tests of cognitive and psychomotor performance in healthy human participants. Pharmacognosy Research 6(1), 12-18. https://doi. org/10.4103/0974-8490.122912

Raghavan A, Shah ZA. 2015. Withania somnifera Improves Ischemic Stroke Outcomes by Attenuating PARP1-AIF-Mediated Caspase-Independent Apoptosis. Molecular Neurobiology 52(3), 1093-1105.

Singh P, Guleri R, Singh V, Kaur G, Kataria H, Singh B, Pati PK. 2015. Biotechnological interventions in Withania somnifera (L.) Dunal. Biotechnology and Genetic Engineering Reviews, 31(1-2), 1-20.

Sözmen EY, Sözmen B, Delen Y, Onat T. 2001. Catalase/superoxide dismutase (SOD) and catalase/paraoxonase (PON) ratios may implicate poor glycemic control. Archives of Medical Research 32(4), 283-287.

Wankhede S, Langade D, Joshi K, Sinha SR, Bhattacharyya S. 2015. Examining the effect of Withania somnifera supplementation on muscle strength and recovery: A randomized controlled trial. Journal of the International Society of Sports Nutrition, 12(1).

Zafar S, Anwar H, Qasim M, Irfan S, Maqbool J, Sajid F, Hussain G. 2020. Calotropis procera (root) escalates functions rehabilitation and attenuates oxidative stress in a mouse model of peripheral nerve injury. Pakistan Journal of Pharmaceutical Sciences 33(6), 2801-2807.

Zhu F, Du B, Xu B. 2018. Anti-inflammatory effects of phytochemicals from fruits, vegetables, and food legumes: A review. Critical Reviews in Food Science and Nutrition 58(8), 1260-1270. 1080/10408398.2016.1251390