Induced mutagenesis in Green gram (Vigna radiata (L.) Wilczek)
Paper Details
Induced mutagenesis in Green gram (Vigna radiata (L.) Wilczek)
Abstract
Induced mutagenesis was carried out in an important protein rich pulse crop (Vigna radiata (L.) Wilczek). The seeds of green gram variety Co-6 were treated with different concentrations of sodium azide. The mutagen treated seeds were sown in the field to observe M1 characteristics. The sodium azide treated seeds were subjected to amino acid analysis. Totally 19 amino acids were recorded in control and sodium azide treated samples. In the process of sodium azide treatments a few amino acids were increased and some amino acids were decreased than control. The M1 parameters such as germination and survival percentage, plant height, days taken for flowering, number of pods/plant, length of pods, number of seeds/pod and hundred seeds weight were decreased with increasing concentrations sodium azide and all the growth parameters showed negative trend when compared to control. The M1 seeds were collected separately based on concentrations of sodium azide and stored for raising next generation after the harvest. The M1 seeds were sown in the field to raise M2 generation, and in M2 population, the different types of chlorophyll and viable mutants were noticed, such as chlorina, xantha, viridis, and viable mutants such as tall, dwarf, leaf, pod and early flowering mutants were noticed in various treatments of sodium azide. In addition with chlorophyll and viable mutants several initial leaflet modifications like trifoliate, tetrafoliate and pentafoliate leaflets had been observed in mutagenic treatment with sodium azide. The present study is a basis for evolving mutant varieties in green gram with altered agronomic traits.
Acharya SA, Srichamroen S, Basu B, Ooraikul Basu T. 2006. Improvement in the nutraceutical properties of fenugreek (Trigonellafoenum graecum L.) Songklanakarin Journal of Science Technology 28(1), 1-9.
Ahloowalia B, Maluszynski M. 2002. Induced mutations- A new paradigm in plant breeding. Euphytica 118, 67-173.
Amol C, Vikhe, Janardhan N, Nehul. 2021. Effect of mutagens (EMS, SA and gamma radiation) on quantitative parameters of [Vigna radiata (L.) Wilczek]. International Journal of Research in Biosciences, Agriculture and Technology 17, 657-664.
Anand Kumar PP, Rajendra Prasad. 2009. Induced Chlorophyll And Morphological Mutations In Mungbean (Vigna radiata L. Wilczek). Legume Research 32(1), 41-45,
Apparao BJ, Auti SG. 2005. Induced leaf morphological mutations in mungbean (Vigna radiata (L.) Wilczek). In: Proceedings of National Conference in Plant Sciences 10-12, Pravaranagar, Padmashri Vitthalrao Vikheatil College, Pravaranagar pp 16-19.
Auti G. 2012. Induced morphological and quantitative mutations in mungbean. Bioremediation, Biodiversity, and Bioavailability. Review Articles.
Awan MA, Konzak CF, Rutger JN, Nilan RA. 1980. Mutagenic effects of sodium azide in rice. United States: N. P.
Dahiya B. 1973. Improvement of mung bean through induced mutations. Indian Journal of Genetics and Plant Breeding 33, 460-468.
Das TR, Baisakh B. 2020. Effectiveness and efficiency of physical and chemical mutagens in green gram Vigna radiata (L.) Wilczek]. E-planet 18(1), 6-12.
Dewanjee S, Sarkar KK. 2018. Evaluation of performance of induced mutants in mung bean [Vigna radiata (L.) Wilczek]. Agriculture Research Communication Centre 41(2), 213-217.
Gehan G, Mostafa. 2011. Effect of Sodium Azide on the Growth and Variability Induction in Helianthus annuus L. International Journal of Plant Breeding and Genetics 5, 76-85.
Gruszka D, Szarejko I, Maluszynski M. 2012. Sodium azide as a mutagen in Plant Mutation Breeding and Biotechnology (CABI International, Wallingford, UK) 159-166.
Harborne AJ. 1998. Phytochemical Methods A Guide to Modern Techniques of Plant Analysis. Springer Science+Business Media B.V.
Ignacimuthu S, Babu CR. 1988. Radio sensitivity of the Wild And Cultivated Urd And Mung Beans. Indian Journal of Genetics and Plant Breeding 48(03), 331-342.
Kaviraj CP, Kiran G, Venugopal RB. 2006. Somatic embryogenesis and plant regeneration from cotyledonary explants of green gram [Vigna radiata (L.) Wilczek.] A recalcitrant grain legume. In vitro Cell Development Biology.-Plant 42, 134-138.
Khalil SK, Muhammas T, Afridi K, Shah SA, Rahman S, Rahman K. 1987. Response of mungbean (Vigna radiata (L.) Wilczek) varieties to different doses of gamma rays and fast neutron irradiation. Sarhad Journal of Agriculture 1, 347-350.
Khan S, Siddiqui BA. 1993. Chlorophyll mutations in (Vigna radiata (L) Wilczek.) mutagenic effectiveness. Pakistan Journal of Botany 2, 161-166.
Kuldeep Singh. 2013. Effectiveness and efficiency of Gamma rays and Ethyl Methane Sulphonate (EMS) in mungbean. Journal of Food Legumes 26 (3 & 4), 25-28.
Lee YI, Lee IS, Lim YP. 2002. Variations in sweet potato regenerates from gamma ray irradiated embryogenic callus. Journal of Plant Biotechnology 4(4), 163-170.
Mahna SK, Garg R, Parvateesam M. 1989. Mutagenic effects of Sodium azide in Black gram. Current Science 58, 582-584.
Mahto CS, Sweta Suman, Niraj Kumar, Shreya Sen. 2018.Mutagenic Effectiveness and Efficiency of EMS and Sodium Azide in Mungbean (Vigna radiata (L.) Wilczek) International Journal of Bio-resource and Stress Management 9(4), 537-540.
Maluszynski M, Nichterlein K, Zanten L, Ahloowalia BS. 2000. Officially released mutant varieties- The FAO/IAEA Database (INIS-XA–291). International Atomic Energy Agency (IAEA): IAEA.
Mohammad Rafiq Wani. 2020. Characterization of Chlorophyll Deficient Mutants in Mungbean (Vigna radiata (L.) Wilczek) Bangladesh Journal of Botany 49(4), 1013-1019,
Muniappan V, Palanivel S, Parvathi S. 2012. Impact of ethyl methane sulphonate on M1 attributes of Groundnut with special emphasis of amino acid profiling. Journal of Advanced Applied Scientific Research 3, 100-108.
Pavadai P, Girija M, Dhanavel D. 2009. Effectiveness and efficiency and biochemical content of physical and chemical mutagens in soya bean (Glycine max (L) Merr.) Journal of Phytolog 1(6), 444-447.
Pepol P, Pepo P. 1989. Preliminary experiment on inducing soybean mutants by fast neutron seed irradiation. Soybean Abstracts. 12(5), 4-7.
Poehlman M. 1991. The mungbean. Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi, India.
Priya Ranjan Tah. 2006.Inducedmacromutation in mungbean (Vigna radiata L.) International journal of botany 2(3), 219-228.
Rukesh M, Abdul Rahuman S, Christine Latitia Packiaraj D. 2017. Impact of gamma irradiation induced mutation on morphological and yield contributing traits of two genotypes of Green gram (Vigna radiata L. Wilczek) Journal of Pharmacognosy and Phytochemistry 6(6), 1229-1234.
Sanjai Gandhi ES, Umavathi Mullainathan L. 2014. Studies on induced chlorophyll mutants in green gram (Vigna radiata (L.) Wilczek) International Journal of Advanced Research 2(2), 00-04.
Sato M, Gaul H. 1967. Effect of Ethyl Methanesulfonate on the fertility of Barley. United Kingdom: N.P.
Singh A, Kumar D. 2009.Genetic parameters and path co-efficient analysis in M4 generation of mungbean (Vigna radiata (L.) Wilczek). Journal of food legumes 22(3), 166-170.
Singh VP, Aggarwal S. 1986.Induced high yielding mutants in cluster bean, Indian Journal of agricultural Science 56, 695-700.
Singh VP, Chaturvedi CN. 1982. Comparative mutagenic effects of EMS and NMC in (Vigna radiata (L.) Wilczak) with DMSO. Indian Journal of Botany 5(1), 54-57.
Singh VP, Chaturvedi SN. 1981. Gamma rays induced male sterility mutation in mung bean. Pulse Crop Newsletter 1, 41-42.
Sofia S, Reddy DM, Reddy KHP, Latha P, Ravindra Reddy B. 2020. Effect of gamma rays, ethyl methane sulphonate and sodium azide on seedling traits, fertility and varietal sensitivity in Mungbean Vigna radiata (L.) Wilczek]. International Journal of Chemical Studies 8(4), 1109-1114.
Sofia S, Reddy DM, Reddy KHP, Latha P, Ravindra Reddy B. 2020. Effect of gamma rays, ethyl methane sulphonate and sodium azide on seedling traits, fertility and varietal sensitivity in Mungbean (Vigna radiata (L.) Wilczek) International Journal of Chemical Studies 8(4), 1109-1114.
Traiq AB, Monika S, Anis M. 2007. Comparative analysis of mitotic aberrations induced by diethyl sulphate (DES) and sodium azide in Viciafaba (L) (Fabaceae): Pakistan Journal of Biological Science 783-787.
Vikhe A, Nehul J. 2020. Studies on mutagenic sensitivity of (Vigna radiata (L.) Wilczek). Tropical Plant Research 7(2), 309-312.
Wani MR, Dar AR, Tak A, Amin I, Shah NH, Rehman R, Baba MY, Raina A, Laskar R, Kozgar MI, Khan S. 2017. Chemo-induced pod and seed mutants in Mungbean (Vigna radiata L.Wilczek). SAARC Journal of Agriculture 15(2), 57-76.
V. Prabakaran, P. Manivel, S. Parvathi, S. Palanivel, 2023. Induced mutagenesis in Green gram (Vigna radiata (L.) Wilczek). J. Biodiv. Environ. Sci., 22(1), 66-73.
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