Induction and characterization of a cellulase overproducing mutant strains of Trichoderma harzianum

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Research Paper 12/05/2023
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Induction and characterization of a cellulase overproducing mutant strains of Trichoderma harzianum

EA. Mahmoud, FH. Nada, AA. Omar, GM. Hassan
Int. J. Micro. Myco.16( 5), 1-13, May 2023.
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Abstract

Nine Trichoderma isolates were isolated from different agricultural soil samples. All the Nine Trichoderma isolates showed positive test for cellulase as revealed by the formation of a clear zone on the screening medium containing Congo red as indicator. The percentage inhibitory effect of all the Nine Trichoderma isolates against growth of R. solani was calculated and ranged from 58 % to 64.9%. The qualitative and antagonistic results showed the isolate FUGT4 had the highest efficiency in antagonistic against R. solani. Molecular phylogenetic analysis was performed based on nucleotide sequences. The results indicate that the isolate FUGT4 are closely related to Trichoderma harzianum, with accession number OL953189. The selected strain was subjected to mutation using UV-radiation and 12 mutants were obtained when exposed to different time periods of radiation.These mutants were tested for their cellulase productivity compared to their wild type and the results showed that, the mutants were produced cellulase 0.921 IU/mL cellulase activities. The ISSR analysis of genomic DNA was performed to detect genetic diversity of these mutants with the wild type by using 6 primers. The mutants and their wild counterparts were tested for their antagonistic potently against to R. solani. The results showed that all mutants induced from the wild type Trichoderma were significantly better than the wild type when tested against R. solani.

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