Welcome to International Network for Natural Sciences | INNSpub

Influences of phytohormones in plant nutrient cultures of in-vitro multiplying banana plantlets alters the cellular biocontents

Research Paper | November 1, 2017

| Download 54

Ikram-ul-Haq, Nazia Parveen Gill, Sajid Ali, Noor e Saba Khaskheli, Abdul Ghaffar

Key Words:

Int. J. Biosci.11( 5), 301-308, November 2017

DOI: http://dx.doi.org/10.12692/ijb/11.5.301-308


IJB 2017 [Generate Certificate]


Banana is an important fruit as well as staple food crop. Its production is decreasing by the infection of a number of inter and intra-cellular pathogens. Banana micropropagation under in-vitro is being a possible way for development of pathogen free plants. In this experiment, banana (cv., Basrai) multiplication was optimized and certain biochemical analysis was performed among the organogenesis and plant multiplication cultures of banana. Maximum plantlets were propagated when meristematic micro-stem cuttings were cultured for organogenesis on MS2 medium supplemented with 10µM 6-Benzyleaminopurine (BAP), 15µM Indole aceticacid (IAA) and solidified with 0.36% phytagel. After 3rd week of incubation, cultures were sub-cultured on MS3 (10 µM BAP, 0.12% phytagel) for shoot induction (for 2- weeks) than for plant or shoot multiplication was carried on MS4 (8µM BAP, 0.2% phytagel) medium (for 4-weeks). Organogenesis process was induced under a specific combination of hormonal (IAA and BAP) stress, while plant multiplication (without IAA) occurs with the elevation of IAA stress due to the adjustment of specific biocontents among the cultured tissues. Likely to that plant biomass, Chl a/Chl b and K+/Cl ratios were increase (p 0.05) while Chl ab/carotenoids and Na+ and K+ ratios (p 0.05) were decreased among organogenesis cultures than plant shoot multiplication cultures. Meanwhile, developed plantlets through this protocol has shown normal plant growth under ex-vitro environment.


Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Influences of phytohormones in plant nutrient cultures of in-vitro multiplying banana plantlets alters the cellular biocontents

AHMAD F, AHMAD I, KHAN MS. 2006. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol. Res 163, 173-181. https://doi.org/10.1016/j.micres.2006.04.001.

Alvard D, Cote F, Teisson C. 1993. Comparison of methods of liquid medium culture for banana micropropagation – Effects of temporary immersion of explants. Plant Cell Tiss. Org. Cult. 32, 55-60. https://doi.org/10.1007/BF00040116.

Bielach A, Hrtyan M, Tognetti VB. 2017. Plants under stress: Involvement of auxin and cytokinin. Int. J. Mol. Sci. 18, 1427. https://doi.org/10.3390/ ijms18071427.

Bojovic BM, Stojanovic J. 2005. Chlorophyll and carotenoid content in wheat cultivars as a function of mineral nutrition. Arch. Biol. Sci 57, 283-290. www.doiserbia.nb.rs/Article.aspx?id=0354-4664 0504283B.

Cosgrove DJ, Li LC, Cho HT, Hoffmann-Benning S, Moore RC, Blecker D. 2002. The growing world of expansins. Plant Cell Physiol. 43, 1436-1444. https://doi.org/10.1093/pcp/pcf180.

Cosgrove DJ. 2015. Plant expansins: Diversity and interactions with plant cell walls. Curr. Opin. Plant Biol 25, 162-172. https://doi.org/10.1016/j.pbi.2015.05.014.

Daniells JW. 1997. Beware the potential hazards of tissue culture. Infomusa 6, 17-18. www.musalit.org/ seeMore.php?id=13918.

Dere S, Gunes T, Sivaci R. 1998. Spectrophotometric Determination of Chlorophyll – A, B and Total Carotenoid Contents of Some Algae Species Using Different Solvents. Turk. J. Bot 22, 13-17.

Escalona M, Lorenzo JC, González B, Daquinta M, González JL, Desjardins Y, Borroto CG. 1999. Pineapple (Ananas comosus L. Merr) micropropagation in temporary immersion systems. Plant Cell Rep. 18, 743-748. https://doi. org/10.1007/s002990050653.

Etienne H, Berthouly M. 2002. Temporary immersion systems for plant microporpagation. Plant Cell Tiss. Org. Cult. 69, 215-231. https://doi.org/10. 1023/A:1015668610465.

Gamborg OL, Miller RA, Ojima K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res 50, 151-158. https://doi.org/10.1016/0014-4827(68)90403-5.

Gaspar T, Kevers C, Penel C, Greppin H, Reid DM, Thorpe TA. 1996. Plant hormones and plant growth regulators in plant tissue culture. In Vitro Cell. Develop. Biol. Plant 32, 272-289. https://doi.org/10.1007/BF02822700.

Georget F, Domergue R, Ferrire N, Cate FX. (2000). Morphohistological study of the different constituents of a banana (Musa AAA, cv. Grande naine) embryogenic cell suspension. Plant Cell Rep 19, 748-754. https://doi.org/10.1007 /s002999900188.

Georgiev V, Schumann A, Pavlov A, Bley T. 2014. Temporary immersion systems in plant biotechnology. Eng. Life Sci 13, 26-38. https://doi. org/10.1002/elsc.201300166.

Glass GK, Wang Y, Buenfeld NR. 1996. An investigation of experimental methods used to determine free and total chloride contents. Cem. Concr. Res 26, 1443-1449. https://doi.org/10.1016/0008-8846(96)00115-9.

Gübbük H, Pekmezci M. 2004. In vitro propagation of some new banana types (Musa spp.). Turk. J. Agric. Forest 28, 355-361.

Hirimburegama K, Gamage N. 1997. Cultivar specificity with respect to in vitro micropropagation of Musca spp. (Banana and plantain). J. Hortic. Sci 158, 205-211. https://doi.org/10.1080/14620316.1997.

Hwang I, Sheen J, Müller B. 2012. Cytokinin Signaling Networks. Annual Review of Plant Biology 63(1), 353-380. https://doi.org/10.1146/annurev-arplant-042811-105503.

Hwang I, Sheen J. 2001. Two-component circuitry in Arabidopsis cytokinin signal transduction. Nature 413, 383-389. https://doi.org/10.1038/35096500.

Hwang SC, Chen CL, Murata GCL, Lin HL. 1984. Cultivation of Banana Using Plantlets from Meristem Culture. Hort Sc 19, 231-233.

Iqbal N, Khan NA, Nazar R, Silva JAT. 2012. Ethylene-stimulated photosynthesis results from increased nitrogen and sulfur assimilation in mustard types that differ in photosynthetic capacity. Environ. Exp. Bot 78, 84-90. https://doi.org/10.1016/j. envexpbot.2011.12.025.

Jambhale ND, Patil STJ, Pawar AS, Waghmode SV. 2001. Effect of number of subcultures on in vitro multiplication of four banana clones. Infomusa 10, 38-39.

Jensen PJ, Hangarter RP, Estelle M. 1998. Auxin transport is required for hypocotyl elongation in light-grown but not dark-grown Arabidopsis. Plant Physiol 116, 455-462. https://doi.org/10.1104/pp.116.2.455.

Jing HC, Schippers JHM, Hille J, Dijkwel PP. 2005. Ethylene-induced leaf senescence depends on age-related changes and OLD genes in Arabidopsis. J. Exp. Bot 56, 2915-2923. https://doi.org/10.1093/jxb/eri287

Jouve L, Gaspar T, Kevers C, Greppin H, Agosti RD. 1999. Involvement of indole-3-acetic acid in the circadian growth of the first internode of Arabidopsis. Planta 209, 136-142. https://doi.org/10. 1007/s004250050615.

Kaur S, Bhutani KK. 2012. Organic growth supplement stimulants for in vitro multiplication of Cymbidium pendulum (Roxb.) Sw. Hortic. Sci    39, 47-52.

Khan NA. 2005. The influence of exogenous ethylene on growth and photosynthesis of mustard (Brassica juncea) following defoliation. Sci. Hortic 105, 499-505. https://doi.org/10.1016/j.scienta.2005.02.004.

Konings H, Jackson MB. 1979. A Relationship between Rates of Ethylene Production by Roots and the Promoting or Inhibiting Effects of Exogenous Ethylene and Water on Root Elongation. Zeit. Pflanzenphysiol 92, 385-397. https://doi.org/10.1016/s0044-328x(79).

Körner C. 1989. The nutritional status of plants from high altitudes. Oecologia 81, 379-391. https://doi.org/10.1007/BF00377088.

Lassmann M, Nosske D. 2013. Dosimetry of 223Ra-chloride: Dose to normal organs and tissues. Europ. J. Nucl. Med. Mol. Imag 40, 207-212. https://doi.org/10.1007/s00259-012-2265-y.

Murashige T, Skoog F. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant 15, 473-497.

Murch SJ, Liu C, Romero RM, Saxena PK. 2004. In vitro culture and temporary immersion bioreactor production of Crescentia cujete. Plant Cell Tiss. Org. Cult 78, 63-68. https://doi.org/10.1023/B:TICU.000002 0397.  01895.3e.

Nazar R, Khan MIR, Iqbal N, Masood A, Khan NA. 2014. Involvement of ethylene in reversal of salt-inhibited photosynthesis by sulfur in mustard. Physiol. Plant 152, 331-344. https://doi.org/10.1111/ppl.12173.

Noh B, Bandyopadhyay A, Peer WA, Spalding EP, Murphy AS. 2003. Enhanced gravi. and phototropism in plant mdr mutants mislocalizing the auxin efflux protein PIN1. Nature 423, 999-1002. https://doi.org/10.1038/nature01716.

Nozue K, Maloof JN. 2006. Diurnal regulation of plant growth. Plant Cell Environ 29, 396-408. https://doi.org/10.1111/j.1365-3040.2005.01489.x.

Paltridge GW, Denholm JV, Connor DJ. 1984. Determinism, senescence and the yield of plants. J. Theor. Biol 110, 383-398. https://doi.org/10.1016/S0022-5193.

Pierik R, Tholen D, Poorter H, Visser EJW, Voesenek LACJ. 2006. The Janus face of ethylene: growth inhibition and stimulation. Trend. Plant Sci 11, 176-183. https://doi.org/10.1016/j.tplants.2006.02.006.

Rayle DL, Cleland RE. 1992. The acid growth theory of auxin-induced cell elongation is alive and well. Plant Physiol. 99, 1271-1274. https://doi.org/10.1104/pp.99.4.1271.

Reinhardt D, Pesce ER, Stieger P, Mandel T, Baltensperger K, Bennett M, Kuhlemeier C. 2003. Regulation of phyllotaxis by polar auxin transport. Nature 426, 255-260. https://doi.org/10.1038/nature02081.

Roels S, Escalona M, Cejas I, Noceda C, Rodriguez R, Canal MJ, Debergh P. 2005. Optimization of plantain (Musa AAB) micropropagation by temporary immersion system. Plant Cell Tiss. Org. Cult. 82, 57-66. https://doi.org/10.1007/s11240-004-67

Scherer RF, Garcia AC, Fraga HPF, Vesco LLD, Steinmacher DA, Guerra MP. 2013. Nodule cluster cultures and temporary immersion bioreactors as a high performance micropropagation strategy in pineapple (Ananas comosus var. comosus). Sci. Hortic 151, 38-45. https://doi.org/10. 1016/j.scienta.2012.11.027.

Vasudevan R, van Staden J. 2011. Cytokinin and explant types influence in vitro plant regeneration of Leopard Orchid (Ansellia africana Lindl.). Plant Cell Tiss. Org Cult 107, 123-129. https://doi.org/10.1007/ s11240-011-9964-0.

Voesenek LACJ, Pierik R, Sasidharan R. 2015. Plant Life without Ethylene. Trend. Plant Sci 20, 783-786. https://doi.org/10.1016/j.tplants.2015.

Vuylsteke D, Swennen R, Wilson GF, De Langhe E. 1988. Phenotypic variation among in-vitro propagated plantain (Musa sp. cultivar “AAB”). Sci. Hortic 36, 79-88. https://doi.org/10.1016/0304-4238(88)90009-X.

WHOFAO. 2002. Food energy, methods of analysis. Food energy methods of analysis and conversion. Fao Food and Nutrition Paper 93. http://doi.org/ISSN 0254-4725.