Inhibition of production of aflatoxin by bioactive compound 2H-furo[2,3-H]-1-benzopyran-2-one isolated from the seeds of Psoralea corylifolia L.

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Research Paper 03/06/2024
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Inhibition of production of aflatoxin by bioactive compound 2H-furo[2,3-H]-1-benzopyran-2-one isolated from the seeds of Psoralea corylifolia L.

V. Lalitha, B. Kiran, Manjula Sheshagiri
Int. J. Biosci.24( 6), 18-30, June 2024.
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Abstract

Forty maize seeds samples collected from different places of Karnataka were subjected for Standard Blotter Method and the seeds borne pathogens were isolated . Among different pathogens isolated, Aflatoxin producing fungi Aspergillus flavus were identified. Aqueous extract of Psoralea corylifolia were prepared at different concentrations and subjected for antifungal activity by poisoned food technique for eight Aspergillus species selected. Among eight Aspergillus species tested, Aspergillus species No. 6 recorded 93.4% inhibition at 50% concentration. Bioactive compound 2H-Furo[2,3-H]-1-benzopyran-2-one, isolated form the seeds of P. corylifolia were teased for antifungal activity against different strains of A. flavus at 250, 500, 750 and1000ppm concentration respectively and the maximum inhibition was observed in 1000ppm concentration and subjected for the isolation of aflatoxin compared  with standard aflatoxin. It was observed that,  Aflatoxin B1(Rf 0.56)  isolated from A.flavus strain no.6  was completely inhibited by bioactive compound  2H-Furo[2,3-H]-1-benzopyran-2-one, confirming the inhibition of Aflatoxin from P. corylifolia seeds.

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