Paper Details

Initiation phase of meristem culture of Prunus avium cvs. “Hajyusefi” and “Zard”

Ebrahim Ganji Moghadam, Mahbube Zamanipour

DOI: https://dx.doi.org/10.12692/ijb/6.2.283-290

Int. J. Biosci. 6(2), 283-290. January, 2015. (PDF)

Abstract:

This trial was carried out to determine the best medium and plant growth regulator concentrations on the initiation of meristem tips from sweet cherry trees cvs. “Hajyusefi” and “Zard”. Meristems were isolated from actively growing plants in the spring and cultured on three kinds of basal media (MS, WPM and QL) supplement with three different concentrations of BA (0, 0.5 and 1 mgL-1), 0.1 mgL-1 GA3 and 0.1 mgL-1 IBA. Explants were soaked in antioxidant solutions (100 mgL-1 ascorbic acid and 150 mgL-1 citric acid for 1 hour). The meristem explants (0.5-0.7 mm) cultured in medium and maintained in a growth room at a 16h – light/8 hr-dark with a light intensity of 2000-3000 lux from white fluorescent light. After six weeks, survival and necrosis ratios and leaf number were studied. Result showed that the highest survival rate and the low necrosis rate (83.3%) was in WPM medium supplemented with 0.5 mgL-1 BA for “Hajyusefi” cultivar. Necrosis occurred more often in vitro condition. Necrosis rate was high in MS (100-83.3%) for “Zard” and “Hajyusefi” cultivars, respectively. The most Leaf number (11) observed in WPM medium supplemented with 0.5 mgL-1 BA in “Hajyusefi” cultivar and the least one (2) was in MS medium without BA application (control) in “Zard” cultivar.