Initiation protocol optimization of (Saccharum officinarum L.) variety US- 718 through micropropagtion techniques
Paper Details
Initiation protocol optimization of (Saccharum officinarum L.) variety US- 718 through micropropagtion techniques
Abstract
The sugarcane variety US-718 experimentally was carried out in changed concentration of BAP and kinetin under established condition of invitro along with replications in complete randomized design. The purpose of the study was to determine the optimum concentration in presence of combination of BAP and kinetin under invitro condition .In MS neutering media the variety US-718 shown maximum result of 90.32% shoot initiation in 3.5#0.5 of shoots per explant in concentrations of 1.5mg/l BAP without kinetin exhibited a better response than other concentration.
Anonymous. 2009. Cetrel and Novozymes to make biogas and electricity from Bagasse. Business wire.
Asare AT, Mensah SK, cheampong SA, Mensah TA. 2017. Screening and Determination of sterilization protocol and Optimum growth regulators for Micropropagtion of sugar cane (Saccharum officinarum L.) in Ghana. The international journal of science and technoledge. Vol 5, Issue 5
Belay Tolera, Mulugeta Diro, Berbew Belew. 2014. In invitro Aseptic culture establishment of sugarcane (Saccharum officinarum L.) Varieties using shoot tip explants. Advances in crop Science and technology, Vol 2, Issue 3, 1000128.
Belet Getnet. 2017. Review on in vitro propagation of sugarcane to advance the value of tissue culture. Agriculture Research and technology open access Journal. Volume 5, Issue 4.
Biradar S, Biradar DP, Patil V, Patil SS, Kambar NS. 2009. Invitro plant regeneration using tip culture in commercial cultivar of sugarcane. Karnataka journal of Agriculture Science 22(1), 21-24.
Danso K E, Azu E, Elgba W, Amootey HM & Klu GYP. 2011. Effective decontamination and subsequent plantlet regeneration of sugarcane (Saccharum officinarum L.) in vitro. International Journal of Integrative Biology 11(2), 90-96.
Directorate of sugarcane Development (DSD). 2013. Status paper on sugarcane. India: Ministry of Agriculture pp 1-16.
FAO. 2014. FAO Statistical year Book: Africa Food and Agriculture pp1-14.
Gandonou C, Erabii T, Abrani J, Idaomar M, Chibi F, and Skali Senhaji N. 2005. Effect of Genotype on callus induction and plant regeneration from leaf explant of sugarcane (Saccharum sp.). African Journal of Biotechnology, vol 4, no 11, pp.1250-1255.
George EF, Machakova I, Zazimalova E. 2008. Plant propagation by tissue culture. Third edition 175-205.
Lakshman P, Geijskes R, Wang L, Smith G, Elliott A. 2006. Developmental and hormonal regulation of direct shoot organogenesis and somatic embryogenesis in sugarcane (Saccharum spp. Interspecfic hybrids) leaf culture. Plant cell Rep 25(10), 1007-1015.
Mekonen, Diro T, Sharma, Negr M. T. 2014. Protocol optimization of invitro propagation of two sugarcane (Saccharum officinarum L) clones grown in Ethiopia. African Journal of Biotechnology 13(12), 1358-1368.
Murashage T, Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco culture. Plant Physiol.15, 473-497.
Paudyal KP, Haq N. 2000. In vitro propagation of pummelo (Citrus grandis L Qsbeck). Invitro cell Dev Biol Plant 36, 511-516.
Sahran VRC, Yadav RC, Chapagain BP. 2004. High frequency plant regeneration from desiccated calli of Indica rice (Oryza Sativa). African Journal of Biotechnology, Vol. 3, no. 5 pp. 256-259, 25.
Shakra Jamil, Rahil Shahzad, Ghulam Mohyuddain Talha, Ghazala Sakhwat, Sajid- ur-Rehman, Razia Sultana, and Muhammad Zaffar Iqbal. 2017. Optimization of protocols for In vitro regeneration of sugarcane (Saccharum officinarum). Hindawi International Journal of Agronomy Volume 2017, Article ID 2089381
Silva RP, Almeida WAB, Souza ES, Filho FAAM. 2006. Invitro organogenesis from adult tissue of Bahia sweet orang (Citrus sinensis). Fruit 61,367-371.
Sime M. 2013. The effect of different cane portions on sprouting growth and yield of sugarcane (Saccharum spp L,). International journal of Science Research publication 3(1), 338-341.
Singh R. 2003. Tissue culture studies of sugarcane. MSc. thesis submitted to the Institute of Engineering and technology.
Tiwari S, Arya A, Kumar S. 2012. Standardizing sterilization protocol and Establishment of Callus culture of Sugarcane for enhanced plant Regeneration in vitro. Research Journal of Botany 7(1), 1-6.
Zahra Jahangir G, Ahmad Nasir I, Ahmad Sial R, Aslam javid M, Hussain T. 2010. Various Hormonal supplementations Activate sugarcane Regeneration Invitro. Journal of Agricultural science, Vol 2 no. 4.
Naseem Khatoon Bhurgri (2021), Initiation protocol optimization of (Saccharum officinarum L.) variety US- 718 through micropropagtion techniques; JBES, V18, N5, May, P127-131
https://innspub.net/initiation-protocol-optimization-of-saccharum-officinarum-l-variety-us-718-through-micropropagtion-techniques/
Copyright © 2021
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0