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Innovative assessment to modulate the toxic effects of CuO-nanoparticles using Trigonella foenum-graecum methanol seed extract in Oreochromis mossambicus

Ghazala Kaukab, Farhat Jabeen, Muhammad Ali, Salma Sultana, Azhar Rasul, Muhammad Asad

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Int. J. Biosci.15(2), 445-455, August 2019

DOI: http://dx.doi.org/10.12692/ijb/15.2.445-455


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Trigonella foenum-graecum has diverse medicinal properties therefore; the present study was aimed to investigate the ameliorative effects of the Trigonella foenum-graecum methanolic seed extract (T-MSE) against the CuO nanoparticles (NPs) induced toxicity in Oreochromis mossambicus. For this purpose, 100 O. mossambicus of 30-45g weight were randomly distributed into 5 groups having 10 fish in each group in duplicates namely control (without any treatment), positive control (treated with waterborne CuO-NPs @ 0.12mg/l), G1, G2, and G3 were treated with waterborne CuO-NPs @ 0.12mg/l plus 16 or 32 or 52 mg/l of T-MSE, respectively for 56 days. Blood sampling was done at three intervals at 7th, 28th and 56th day of exposure. It was found that T-MSE remarkably ameliorated the toxic effects of CuO-NPs in G3 with high T-MSE dose (52 mg/l) in the hematology of fish sampled at 28th and 56th day of exposure while, at 7th day of exposure less improvement was observed as  compared with positive control group. It was also observed that the toxic effect of CuO-NPs in G3 was less ameliorated at 28th day of exposure.  There were significant differences in T-MSE treated groups (G1, G2 and G3) having most prominent shielding effects of T-MSE in G3 at 28th and 56th  day of exposure in O. mossambicus compared with the positive control, G1 and G2 groups (p<0.05). It was concluded that T-MSE had prominent ameliorative effects on hematology against the toxic effects of water-borne CuO nanoparticles in O. mossambicus.


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Innovative assessment to modulate the toxic effects of CuO-nanoparticles using Trigonella foenum-graecum methanol seed extract in Oreochromis mossambicus

Abdel-Khalek AA, Kadry MA, Badran SR, Marie MA. 2015. Comparative toxicity of copper oxide bulk and nano particles in Nile tilapia Oreochromis niloticus: biochemical and oxidative stress. The Journal of Basic & Applied Zoology 72, 43-57.

Chow JCL. 2018. Recent Progress in Monte Carlo Simulation on Gold Nanoparticle Radio-sensitization. AIMS Biophysics 5, 231-244.

Cristea CM, Tertis R, Galatus. 2017. Magnetic nanoparticles for antibiotics detection. Nanomaterials 7, 119-122.

Deepa N, Usha R, Anbarasu A, Anuanandhi K, Karnan P, Elumalai K. 2018. Destaining Potential of Bacterial Lipase enzyme Isolated from providencia rettgeri inhabiting the fresh-water fish Mystus bleekeri. World Journal of Pharmaceutical Research 7, 2101-13.

Hamid NH, Shirzad H. 2013Toxicity and safety of medicinal plants. Journal of Herbs and Medicines Pharmacology 2(2), 21-22.

Islam M, Shehzadi N, Salman M, Zahid F, Qamar S, Danish MZ, Bukhari NI. 2019. Metabolomics and marker-based stability studies of methanol extract of seeds of Syzygium cumini L. Pakistan Journal of Pharmaceutical Sciences 32(2), 499-504.

Jahanbakhshi A, Hedayati A, Pirbeigi A. 2015. Determination of acute toxicity and the effects of sub-acute concentrations of CuO nanoparticles on blood parameters in Rutilus rutilus. Nanomedicinal Journal 2(3), 195-202.

Khan RA, Khan MR, Sahreen S. 2012. Protective effect of Sonchus asper extracts against experimentally induced lung injuries in rats: A novel study. Experimental and Toxicological Pathology 64(7–8), 725-731. http://dx.doi.org/10.1016/j.etp.2011.01.007

Kumar SV, Bafana AP, Pawar P, Faltane M, Rahman A, Dahoumane SA, Jeffryes CS. 2019. Optimized Production of Antibacterial Copper Oxide Nanoparticles in a Microwave-Assisted Synthesis Reaction Using Response Surface Methodology. Colloids and Surfaces A: Physicochemical and Engineering Aspects 2(21), 12-25.  https://doi.org/10.1016/j.colsurfa.2019.04.063

Kusumaa IW, Arunga MET, Kim SY. 2014. Food Science and Human Welfare 3, 191-196.

Kusumaa R, Reddy P, Bhashar N, Venkatesh S. 2012. Phytochemical and Pharmacological studies of Pandanus odoratissimus Linn. International Phytochemical Pharmacology 2(4), 171-174.

Noureen A, Jabeen F, Tabish TA, Ali M, Iqbal R, Yaqub S, Chaudhry SA. 2019. Histopathological changes and antioxidant responses in common carp (Cyprinus carpio) exposed to copper nanoparticles. Drug and Chemical Toxicology http://dx.doi.org/10.1080/01480545.2019.1606233

Noureen A, Jabeen F, Tabish TA, Yaqub S, Ali M, Chaudhry AS. 2018. Assessment of copper nanoparticles (Cu-NPs) and copper (II) oxide (CuO) induced hemato-and hepatotoxicity in Cyprinus carpio. Nanotechnology 29(14), 144003-17. https://doi.org/10.1088/1361-6528/aaaaa7

OECD. 2000. Guidance Document on Acute Oral Toxicity. Environmental Health and Safety Monograph Series on Testing and Assessment No.24.

Patel DK, Dhanabal SP. 2013. Development and optimization of bioanalytical parameters for the standardization of Trigonellafoenum-graecum. Journal of Acute Disease 2(2), 137-139. http://dx.doi.org/10.1016/S2221-6189(13)60114-6

Remya VR, Abitha VK, Rajput PS, Ajay V, Rane, Dutta A. 2017. Silver nanoparticles green synthesis: A mini review. Chemistry International 3(2), 165-171.

Subhashini N, Thangathirupathi A, Lavanya  N. 2011. Antioxidant activity of trigonella foenum graecum using various in vitro and ex vivo models. International Journal of Pharmacy and Pharmaceutical Sciences 3(2), 96-102.

Wu T, Tang M. 2018. Review of the effects of manufactured nanoparticles on mammalian target organs. Journal of Applied Toxicology 38(1), 25-40.


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