Innovative assessment to modulate the toxic effects of CuO-nanoparticles using Trigonella foenum-graecum methanol seed extract in Oreochromis mossambicus

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Research Paper 01/08/2019
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Innovative assessment to modulate the toxic effects of CuO-nanoparticles using Trigonella foenum-graecum methanol seed extract in Oreochromis mossambicus

Ghazala Kaukab, Farhat Jabeen, Muhammad Ali, Salma Sultana, Azhar Rasul, Muhammad Asad
Int. J. Biosci.15( 2), 445-455, August 2019.
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Trigonella foenum-graecum has diverse medicinal properties therefore; the present study was aimed to investigate the ameliorative effects of the Trigonella foenum-graecum methanolic seed extract (T-MSE) against the CuO nanoparticles (NPs) induced toxicity in Oreochromis mossambicus. For this purpose, 100 O. mossambicus of 30-45g weight were randomly distributed into 5 groups having 10 fish in each group in duplicates namely control (without any treatment), positive control (treated with waterborne CuO-NPs @ 0.12mg/l), G1, G2, and G3 were treated with waterborne CuO-NPs @ 0.12mg/l plus 16 or 32 or 52 mg/l of T-MSE, respectively for 56 days. Blood sampling was done at three intervals at 7th, 28th and 56th day of exposure. It was found that T-MSE remarkably ameliorated the toxic effects of CuO-NPs in G3 with high T-MSE dose (52 mg/l) in the hematology of fish sampled at 28th and 56th day of exposure while, at 7th day of exposure less improvement was observed as  compared with positive control group. It was also observed that the toxic effect of CuO-NPs in G3 was less ameliorated at 28th day of exposure.  There were significant differences in T-MSE treated groups (G1, G2 and G3) having most prominent shielding effects of T-MSE in G3 at 28th and 56th  day of exposure in O. mossambicus compared with the positive control, G1 and G2 groups (p<0.05). It was concluded that T-MSE had prominent ameliorative effects on hematology against the toxic effects of water-borne CuO nanoparticles in O. mossambicus.


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