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Insecticidal activities of Cymbopogon nordus (Linn.) wholeplant ethyl acetate extract against lymphatic filariasis vector with study on non-target organisms

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Research Paper 01/06/2019
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Insecticidal activities of Cymbopogon nordus (Linn.) wholeplant ethyl acetate extract against lymphatic filariasis vector with study on non-target organisms

Ikram Ilahi, Muhammad Attaullah, Abdur Rahim, Muhammad Anwar Sajad, Shariat Ullah, Shah Zaman, Gul Rahim, Hazrat Ali
Int. J. Biosci.14( 6), 311-322, June 2019.
Certificate: IJB 2019 [Generate Certificate]
Key: AdulticidalBiochemical parametersHaematological parametersLarvicidalPupicidal

Abstract

This study aimed to evaluate the insecticidal activities of Cymbopogon nardus-whole plant ethyl acetate extract (CNEAE) against the lymphatic filariasis vector, Culex quinquefasciatus. The extract caused insignificantly higher (P>0.05) mortality of early instar larvae as compared to late instar larvae. The 24-hour LC50 values of CNEAE against Cx. quinquefasciatus 2nd, 3rd and 4th instar larvae were 439.1ppm, 451.8ppm and 665.4ppm, respectively. The 24-hour LC50 value of CNEAE against pupae (LC50= 2740.4ppm) was several times higher than the LC50 values of this extract against larvae. During adulticidal activity, the KDT50 value at the highest concentration (1.25%) of CNEAE during CDC bottle bioassay was 286.4 minutes. The KDT50 value at the highest concentration (0.138mg/cm2) of CNEAE during filter paper impregnation bioassay was 218.2 minutes. During the study on non-target insect, Libellula fulva, CNEAE caused no mortality up to concentration of 500ppm, however at highest concentration (1000ppm), CNEAE caused 4.6±1.6 mortality in L. fulva nymphs. During this study, grass carp fish, Ctenopharingodon idella was exposed to the LC50 of CNEAE, estimated against Cx. quinquefasciatus 4th instar larvae. The CNEAE caused no behavioral changes or mortality in C. idella. During this study, high dose (2000mg/kg. b.w/oral) of CNEAE was orally administered to male rabbits. The CNEAE caused no increase in the serum levels of ALT, AS, ALP and creatinine. Similarly, the CNEAE caused no abnormal change in the blood cells counts and haemoglobin concentration.

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